| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
It is well known that O-GlcNAcylation of nuclear and cytoplasmic proteins, such as transcription factors, induces differentiation and proliferation of the cell and furthermore, such modification regulates their proteostasis. We noticed that O-GlcNAcylation of proteins plays an important role in their functions, however there are many difficulties in their biochemical and biological analyses. In this project, we established many kinds of analytical methods to clarify the meaning of O-GlcNAc modification of proteins through high sensitive identification of O-GlcNAcylated proteins, identification of GlcNAc-modified residues, establishment of antibodies specific for GlcNAc-modified residues, observation of dynamic protein modification by O-GlcNAc, and identification interacting partner protein with O-GlcNAcylated proteins.
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