| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
Living organisms are constantly exposed to toxic organic molecules, and cells have a mechanism to excrete them as a defensive measure. The molecular entity of this efflux mechanism is the membrane protein, the "efflux transporter". In this study, we analyzed the structure of MATE, a multidrug efflux transporter, focusing on the efflux mechanism of organic molecules. For eukaryotic MATE, we determined the structure of the outward opening state of the MATE transporter AtDTX14, from Arabidopsis, and verified the transport mechanism by structure-based mutational analysis. We also analyzed the structure of VcmN, a MATE from a pathogenic bacterium. The results of this structural analysis suggested that VcmN extrudes the substrate by H+-motive force-dependent TM1 bending.
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