| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2019: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2017: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Outline of Final Research Achievements |
The charge-density analysis can provide the information about the density distribution of outer shell electrons, the atomic charge and the position of hydrogen atoms, which is essential for understanding the physical and chemical properties. However, few reports are available on the analysis in protein molecules. In this research, we performed the charge density analysis of green fluorescent protein (GFP) at resolution of 0.78 A. The precise electronic structure gives information about the valence electron distribution, the atomic charge and the weak interaction. Meanwhile, neutron crystallography using perdeuteration protein is effective method for determining the positions of hydrogen atoms. We evaluated the influence of physical properties and structure by perdeuteration and performed the neutron diffraction experiment. A series of studies quantified the weak interactions between the chromophore and the protein environment and enable us to explain the properties of GFP.
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