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Mixed-valence state of oxygen-evolving complex in photosynthetic photosystem II

Research Project

Project/Area Number 17H03645
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionOsaka City University

Principal Investigator

Kamiya Nobuo  大阪市立大学, 複合先端研究機構, 名誉教授 (60152865)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2017: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywords光化学系II / 光合成 / 水分解酸素発生 / 金属クラスター / 混合原子価状態 / SPring-8 / 金剛原子価 / タンパク質 / X線結晶構造解析 / 蛋白質 / 混合原子価 / 水分解 / 酸素発生
Outline of Final Research Achievements

Photosystem II (PSII) progresses the first reaction of photosynthesis, in which the oxygen-evolving complex (Mn4Ca cluster) splits two water molecules and produces one molecular oxygen. By crystal structure analyses of PSII utilizing anomalous dispersion effect of Mn atoms, valence distributions of the four Mn atoms denoted from Mn1 to Mn4 in the Mn4Ca clusters were established. The results showed that Mn1 altered it valence number as III and II for two PSII monomers in a crystallographic asymmetric unit. Based on the new knowledge obtained for the S1 and S0 states of the Kok cycle model, changes in redox potential were presumed for the hole oxygen-evolving reaction, and a reaction mechanism of PSII was newly proposed.

Academic Significance and Societal Importance of the Research Achievements

光合成は、二酸化炭素と水から、地球上のほとんど全ての生物が生きるための糧となる炭水化物と酸素を提供している。光化学II(PSII)は、光合成の初発過程を担い、また光合成反応の半分にあたる水分解・酸素発生反応を触媒している酵素である。その反応機構の解明には、光合成の本質に迫る学術的な意義と共に、現在の我々にとって喫緊の課題となっている環境問題や将来の食料問題を解決するための基礎知識となる点で大きな社会的な意義も持っている。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (35 results)

All 2019 2018 2017

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 2 results) Presentation (29 results) (of which Int'l Joint Research: 8 results,  Invited: 7 results)

  • [Journal Article] The crystal structure of homoserine dehydrogenase complexed withl-homoserine and NADPH in a closed form2019

    • Author(s)
      Akai Shota、Ikushiro Hiroko、Sawai Taiki、Yano Takato、Kamiya Nobuo、Miyahara Ikuko
    • Journal Title

      The Journal of Biochemistry

      Volume: 165 Issue: 2 Pages: 185-195

    • DOI

      10.1093/jb/mvy094

    • NAID

      40021810011

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient hydrogen production using photosystem I enhanced by artificial light harvesting dye2019

    • Author(s)
      Nagakawa Haruki、Takeuchi Ayano、Takekuma Yuya、Noji Tomoyasu、Kawakami Keisuke、Kamiya Nobuo、Nango Mamoru、Furukawa Rei、Nagata Morio
    • Journal Title

      Photochemical & Photobiological Sciences

      Volume: 18 Issue: 2 Pages: 309-313

    • DOI

      10.1039/c8pp00426a

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Enhancement of Photocurrent by Integration of an Artificial Light-Harvesting Antenna with a Photosystem I Photovoltaic Device2019

    • Author(s)
      Takekuma Yuya、Nagakawa Haruki、Noji Tomoyasu、Kawakami Keisuke、Furukawa Rei、Nango Mamoru、Kamiya Nobuo、Nagata Morio
    • Journal Title

      ACS Applied Energy Materials

      Volume: - Issue: 6 Pages: 3986-3990

    • DOI

      10.1021/acsaem.9b00349

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel Mechanism of Cl-Dependent Proton Dislocation in Photosystem II (PSII): Hybrid Ab initio Quantum Mechanics/Molecular Mechanics Molecular Dynamics Simulation2019

    • Author(s)
      Nakamura Atsushi、Kang Jiyoung、Terada Ryu-ichiro、Kino Hiori、Umena Yasufumi、Kawakami Keisuke、Shen Jian-Ren、Kamiya Nobuo、Tateno Masaru
    • Journal Title

      Journal of the Physical Society of Japan

      Volume: 88 Issue: 8 Pages: 084802-084802

    • DOI

      10.7566/jpsj.88.084802

    • NAID

      40021971929

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Synthesis and characterization of chemically-reactive solubilization surfactants for membrane proteins and preparation of membrane protein hydrogel microfibers2019

    • Author(s)
      Taniguchi Aki、Koeda Shuhei、Noji Tomoyasu、Kawakami Keisuke、Sumito Natsumi、Dewa Takehisa、Itoh Shigeru、Kamiya Nobuo、Mizuno Toshihisa
    • Journal Title

      Colloid and Interface Science Communications

      Volume: 32 Pages: 100199-100199

    • DOI

      10.1016/j.colcom.2019.100199

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Theoretical Elucidation of Geometrical Structures of the CaMn4O5 Cluster in Oxygen Evolving Complex of Photosystem II Scope and Applicability of Estimation Formulae of Structural Deformations via the Mixed-Valence and Jahn?Teller Effects2019

    • Author(s)
      Shoji Mitsuo、Isobe Hiroshi、Yamanaka Shusuke、Umena Yasufumi、Kawakami Keisuke、Kamiya Nobuo、Yamaguchi Kizashi
    • Journal Title

      Advances in Quantum Chemistry

      Volume: 78 Pages: 307-451

    • DOI

      10.1016/bs.aiq.2018.05.003

    • ISBN
      9780128160848
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] Mixed-Valence State of the Oxygen-Evolving Complex in Photosystem II, Determined by Utilization of X-ray Anomalous Dispersion Effect2019

    • Author(s)
      Nobuo, Kamiya; Keisuke, Kawakami; Ayako, Tanaka
    • Organizer
      International Symposium on Diffraction Structural Biology ISDSB2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ROLES OF RED-CHLOROPHYLLS IN LHIGT-HARVESTING OF CYNANOBACTERIAL PSI, AS REVEALED BY ULTRAFAST SPECTROSCOPY2019

    • Author(s)
      Daisuke Kosumi, Hayata Yamamoto, Shuhei Yoshino, Masato Ueno, Keisuke Kawakami, and Nobuo Kamiya
    • Organizer
      International Conference on Solar Fuel ISF3-young
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ENERGY TRANSPORT IN THE PHYCOBILISOME-PHOTOSYSTEM SUPERCOMPLEX FROM THERMOPHILIC CYANOBACTERIA2019

    • Author(s)
      Yuma Hirota, Hiroki Serikawa, Keisuke Kawakami, and Nobuo Kamiya, Daisuke Kosumi
    • Organizer
      International Conference on Solar Fuel ISF3-young
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] STRUCTURAL STUDY OF PHYCOCYANIN COMPLEX IN PHYCOBILISOME BY CRYO-ELECTRON MICROSCOPY2019

    • Author(s)
      Keisuke Kawakami, Tasuku Hamaguchi, Yuhei O Tahara, Junko Shiomi, Daisuke Kosumi, Nobuo Kamiya, Makoto Miyata, Koji Yonekura
    • Organizer
      International Conference on Solar Fuel ISF3-young
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] EXCITASION ENERGY TRANSFER DYNAMICS OF CYANOBACTERIAL PHOTOSYNTHETIC ANTENNA PHYCOBILISOME2019

    • Author(s)
      Daisuke Kosumi, Yuma Hirota, Hiroki Serikawa, Keisuke Kawakami, and Nobuo Kamiya
    • Organizer
      International Conference on Artificial Photosynthesis-2019 ICARP2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] VALENCE FREXIBILITY OF MANGANESE ATOMS IN THE OXYGEN-EVOLVING COMPLEX OF PHOTOSYSTEM II2019

    • Author(s)
      Nobuo Kamiya, Keisuke Kawakami, and Ayako Tanaka
    • Organizer
      International Conference on Artificial Photosynthesis-2019 ICARP2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] STRUCTURAL STUDY OF PHYCOCYANIN COMPLEX IN PHYCOBILISOME INVOLVED IN EXCITATION ENERGY TRANSFER DYNAMICS2019

    • Author(s)
      Keisuke Kawakami, Tasuku Hamaguchi, Yuhei Tahara, Zyunko Shiomi, Daisuke Kosumi, Nobuo Kamiya, Makoto Miyata, Koji Yonekura
    • Organizer
      International Conference on Artificial Photosynthesis-2019 ICARP2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 光合成超複合体における光エネルギー移動機構の解明に向けた取組み2019

    • Author(s)
      川上恵典, 田原悠平, 浜口祐, 小澄大輔, 神谷信夫, 米倉功治, 宮田真人
    • Organizer
      光合成学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] アクリルアミド系高分子修飾PG-surfactantの膜蛋白質可溶化挙動の評価2019

    • Author(s)
      嶋本 太郎, 川上 惠典, 出羽 毅久, 神谷 信夫, 伊藤 繁, 水野 稔久
    • Organizer
      第68回高分子学会年次大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 光合成・光化学系IIの結晶の同型性と酸素発生クラスターのMn原子の価数2019

    • Author(s)
      神谷信夫
    • Organizer
      X線の会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 界面活性剤フリーな膜蛋白質利用手法の検討2019

    • Author(s)
      嶋本太郎、川上惠典、神谷信夫、水野稔久
    • Organizer
      CSJ化学フェスタ2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] 生命科学の飛躍のために一層深まる蛋白質結晶学の役割-光合成・光化学系IIのより確かな構造を求めて2019

    • Author(s)
      神谷信夫
    • Organizer
      結晶学会年会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 光合成で働く光化学系II・酸素発生クラスターの構造とMn原子の価数2019

    • Author(s)
      神谷信夫
    • Organizer
      触媒学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 藻類由来光合成超複合体における光エネルギー移動機構の解明に向けて2018

    • Author(s)
      川上恵典、廣田悠真、田原愁平、塩見潤子、眞岡孝至、瀧尾進、宮田真人、神谷信夫、小澄大輔
    • Organizer
      カロテノイド研究談話会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 多孔質ガラス板内部における光化学系Iから水素発生助触媒である白金ナノ粒子への光誘起電子移動反応2018

    • Author(s)
      平野誠人、野地智康、川上恵典、神哲郎、近藤政晴、大岡宏造、神谷信夫
    • Organizer
      生物物理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 膜蛋白質をナノ繊維内部に固定化した不織布の作製と評価2018

    • Author(s)
      嶋本太郎、野地智康、川上惠典、出羽毅久、神谷信夫、伊藤繁、水野稔久
    • Organizer
      高分子学会討論会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ピコ秒時間分解蛍光分光を用いた光合成超複合体のエネルギー伝達過程の解明2018

    • Author(s)
      廣田悠真、藤本将吾、川上恵典、神谷信夫、小澄大輔
    • Organizer
      分子科学討論会
    • Related Report
      2018 Annual Research Report
  • [Presentation] フィコビリソーム・光化学系II超複合体における光捕集及びエネルギー散逸過程の解明2018

    • Author(s)
      廣田悠真、藤本将吾、川上恵典、神谷信夫、小澄大輔
    • Organizer
      光化学討論会
    • Related Report
      2018 Annual Research Report
  • [Presentation] シアノバクテリア由来PSIにおける励起状態ダイナミクスの励起エネルギー依存性2018

    • Author(s)
      芳野修平、川上恵典、神谷信夫、小澄大輔
    • Organizer
      光合成セミナー
    • Related Report
      2018 Annual Research Report
  • [Presentation] シアノバクテリア及び紅藻類由来チラコイド膜における分光学特性の比較2018

    • Author(s)
      廣田悠真、川上恵典、神谷信夫、瀧尾進、小澄大輔
    • Organizer
      光合成セミナー
    • Related Report
      2018 Annual Research Report
  • [Presentation] シアノバクテリア光合成におけるたんぱく質間相互作用の解明を目指した生化学及び分光学的アプローチ2018

    • Author(s)
      廣田悠真、藤本将吾、川上恵典、神谷信夫、小澄大輔
    • Organizer
      光合成セミナー
    • Related Report
      2018 Annual Research Report
  • [Presentation] 化学反応性膜蛋白質可溶化試薬を用いた膜蛋白質の可溶化とその後のゲル化による膜蛋白質内包ゲルファイバーの作製と評価2018

    • Author(s)
      嶋本太郎,谷口明希,野地智康,川上恵典,出羽毅久,神谷信夫,伊藤繁,水野稔久
    • Organizer
      高分子学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] cyt c6/光化学系I/白金ナノ粒子複合体による光誘起水素発生2018

    • Author(s)
      平野誠人, 野地智康, 川上恵典, 神哲郎, 近藤政晴, 大岡宏造, 神谷信夫
    • Organizer
      ナノ学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 光化学系IIとガラスナノ空間を利用した高効率キノン光還元系の構築2018

    • Author(s)
      野地智康,池田有佑,川上恵典,神哲郎,神谷信夫
    • Organizer
      ナノ学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 天然光合成における水分解酸素発生光化学系IIの構造と機能2018

    • Author(s)
      神谷信夫
    • Organizer
      化学会中長期企画
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Attempt to prepare magnetically oriented microcrystal array of photosystem II for neutron diffraction2018

    • Author(s)
      Hiroki Tabuchi, Keisuke Kawakami, Fumiko Kimura, Tsunehisa Kimura, Nobuo Kamiya
    • Organizer
      植物生理学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Bond distances in the intact Mn4CaO5-cluster of oxygen-evolving photosystem II based on coordinate error2017

    • Author(s)
      Keisuke Kawakami, , Naoto Inohara, Nobuo Kamiya
    • Organizer
      Japan-France Joint Workshop on the Structure and Function of Photosystem II
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 光合成から人工光合成へ ー電気(電子)と化合物のはなしー2017

    • Author(s)
      神谷信夫
    • Organizer
      大分スーパーサイエンスコンソーシアム「科学講演会」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 光化学系IIの中性子結晶構造解析に向けて2017

    • Author(s)
      田淵大輝,川上恵典,神谷信夫
    • Organizer
      結晶学会
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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