| Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Outline of Final Research Achievements |
Mitochondrial respiratory complex I, III and IV organized into super complex convert energy from a series of electron transfer into a proton electrochemical gradient, harvested by complex V for synthesis of ATP. The structure and functions of the super complex and each respiratory complex, purified from bovine heart to retain their original structure and functions, were compared. In all crystals reported to date, bovine complex IV exists as a dimer. Based on the structural and functional comparison of the monomer and dimer, we propose that the monomer is the activated form. Two cardiolipins are located at the interface region in the super complex. The visible resonance Raman spectra of purified reactive super complex and mixture of its individual components enabled us to discuss the detailed active site structures, but no detectable differences were observed. This indicates that the formation of a super complex did not markedly change the structure or electronic state of heme.
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