| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2017: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Outline of Final Research Achievements |
Branched-chain amino acids, also known as BCAAs, are important nutrients involved in a variety of physiological functions and diseases. The branched-chain amino acids incorporated into the cell are transported into the mitochondria for metabolism, but the mechanism by which they are transported into the mitochondria has been unclear. We have developed a new fluorescent biosensor, OLIVe, for imaging branched-chain amino acid concentrations in the cytosol and mitochondria, allowing us to examine spatiotemporal dynamics of the amino acids inside a single living cell. This technique was used to screen mitochondrial branched-chain amino acid transporters, and three candidate genes were identified.
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