| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Outline of Final Research Achievements |
The replication initiation complex in E. coli consists of the origin DNA, the initiator protein DnaA, the DNA-bending protein IHF and so on. This complex promotes unwinding of the origin DNA and loading of the replicative helicase DnaB to the unwound DNA region. In the research #1 “Analysis of loading mechanism of DnaB helicase to the origin DNA”, I mainly revealed the molecular mechanism of the unwinding of origin DNA by DnaA and the interaction mechanism between DnaA and DnaB. Also, in the research #2 “Analysis of functional mechanisms of the regulatory systems for DnaA”, I mainly revealed dynamic mechanism of DnaA complex formed in the DARS system which activates DnaA in a timely manner during the cell cycle.
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