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Optical control of number of motor proteins transporting mitochondria investigated by fluctuation analysis

Research Project

Project/Area Number 17H03659
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionTohoku University

Principal Investigator

HAYASHI Kumiko  東北大学, 工学研究科, 准教授 (00585979)

Co-Investigator(Kenkyū-buntansha) 角田 聡  名古屋工業大学, 工学(系)研究科(研究院), 研究員 (00598857)
佐川 貴志  国立研究開発法人情報通信研究機構, 未来ICT研究所フロンティア創造総合研究室, 研究員 (80749903)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywords光操作 / タンパク質分子モーター / 細胞内輸送 / 非侵襲力測定 / 分子モーター / 光物性 / 非平衡統計力学
Outline of Final Research Achievements

Organelles are transported by motor proteins kinesin and dynein. The number of such motors can be estimated by the non-invasive force measurement method based on non-equilibrium statistical mechanics. In the research, we aim to artificially increase or decrease the number of motors by optical control, and check the validity of the non-invasive force measurement method. Because of the specific problem related to mitochondrial transport, we could not change the number of motors by optical control, we succeeded in decreasing the number by the addition of inhibitor of dynein, and checked the validity of the non-invasive force measurement method.

Academic Significance and Societal Importance of the Research Achievements

細胞内は複雑な環境にあり、その中で機能するタンパク質の物理的性質を調べることは難しい。そのような状況の中、非侵襲力測定法は細胞内での物理計測のツールとして発展してきた。非侵襲力測定法の主なターゲットは神経細胞軸索中の細胞小器官輸送である。アルツハイマー病、パーキンソン病などの神経疾患で細胞小器官輸送の欠陥が報告されている。将来、神経細胞軸索中の細胞小器官輸送の解明は神経疾患研究の発展に貢献すると期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (27 results)

All 2020 2019 2018 2017

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (22 results) (of which Int'l Joint Research: 8 results,  Invited: 7 results)

  • [Journal Article] Physical parameters describing neuronal cargo transport by kinesin UNC-1042019

    • Author(s)
      Hayashi Kumiko、Matsumoto Shiori、Miyamoto Miki G.、Niwa Shinsuke
    • Journal Title

      Biophysical Reviews

      Volume: 11 Issue: 3 Pages: 471-482

    • DOI

      10.1007/s12551-019-00548-9

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Investigation of multiple-dynein transport of melanosomes by non-invasive force measurement using fluctuation unit χ2019

    • Author(s)
      Hasegawa Shin、Sagawa Takashi、Ikeda Kazuho、Okada Yasushi、Hayashi Kumiko
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 5099-5108

    • DOI

      10.1038/s41598-019-41458-w

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Application of the fluctuation theorem to motor proteins: from F1-ATPase to axonal cargo transport by kinesin and dynein.2018

    • Author(s)
      K. Hayashi
    • Journal Title

      Biophysical Reviews

      Volume: 10 Issue: 5 Pages: 1311-1321

    • DOI

      10.1007/s12551-018-0440-5

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of the fluctuation theorem for noninvasive force measurement in living neuronal axons2018

    • Author(s)
      Hayashi Kumiko、Tsuchizawa Yuta、Iwaki Mitsuhiro、Okada Yasushi
    • Journal Title

      Molecular Biology of the Cell

      Volume: 29 Issue: 25 Pages: 3017-3025

    • DOI

      10.1091/mbc.e18-01-0022

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Non-invasive force measurement reveals the number of active kinesins on a synaptic vesicle precursor in axonal transport regulated by ARL-82018

    • Author(s)
      Kumiko Hayashi, Shin Hasegawa, Takashi Sagawa, Sohei Tasaki and Shinsuke Niwa
    • Journal Title

      Physical Chemistry Chemical Physics

      Volume: 20 Issue: 5 Pages: 3403-3410

    • DOI

      10.1039/c7cp05890j

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Effect of the dynein inhibitor ciliobrevin on the number of force producing units transporting synaptic cargos2020

    • Author(s)
      Kumiko Hayashi, Miki G. Miyamoto, Shinsuke Niwa
    • Organizer
      Biophysical Society 64th Annual Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Motility characteristics of human KIF1A mutants in hippocampal neurons in relation to hereditary spastic paraplegia2020

    • Author(s)
      Shiori Matsumoto, Kyoko Chiba, Shinsuke Niwa, Kumiko Hayashi
    • Organizer
      Biophysical Society 64th Annual Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 神経細胞軸索輸送の分子モーターカウンティング2019

    • Author(s)
      林 久美子
    • Organizer
      日本生物物理学会第57回年会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 遺伝性痙性対麻痺を引き起こす変異型ヒトKIF1Aの運動特性2019

    • Author(s)
      松本 栞里, 千葉 杏子, 丹羽 伸介, 林 久美子
    • Organizer
      日本生物物理学会第57回年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] KIF1A/UNC-104によるシナプス小胞前駆体輸送の数理モデル2019

    • Author(s)
      佐々木 瞭, 品川 遼太, 佐々木 一夫, 丹羽 伸介, 林 久美子
    • Organizer
      日本生物物理学会第57回年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ゆらぎを利用した低侵襲な力測定による神経細胞軸索輸送の解明2019

    • Author(s)
      林 久美子
    • Organizer
      第92回日本生化学会大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 分子モーターの軸索輸送速度の極値統計解析2019

    • Author(s)
      直井拓磨、名木野貴美子、佐々木一夫、丹羽伸介、林久美子
    • Organizer
      第9回日本生物物理学会関東支部会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ベイズ統計を用いた神経細胞軸索輸送の 自動粒子追跡2019

    • Author(s)
      稗田康洋 、大久保潤 、林久美子
    • Organizer
      第9回日本生物物理学会関東支部会
    • Related Report
      2019 Annual Research Report
  • [Presentation] iPSニューロンのための物理解析、物理モデルのためのiPSニューロン2019

    • Author(s)
      林 久美子
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Investigation of multiple-dynein transport of melanosomes by non-invasive force measurement using the fluctuation theorem2019

    • Author(s)
      Shin Hasegawa, Takashi Sagawa, Kazuho Ikeda, Yasushi Okada, and Kumiko Hayashi
    • Organizer
      Biophysical Society 63rd Annual Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 蛍光プローブやナノ粒子を用いたタンパク質分子モーターの1分子観察2018

    • Author(s)
      林 久美子
    • Organizer
      化学工学会 第50回秋季大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 遺伝性痙性対麻痺を引き起こす変異型ヒトKIF1Aの神経細胞内Run-time分布2018

    • Author(s)
      松本 栞里, 丹羽 伸介, 林 久美子
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ゆらぎを利用した非侵襲力測定の軸索輸送動画解析への応用2018

    • Author(s)
      稗田 康洋, 佐川 貴志, 千葉 杏子, 林 久美子
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Non-invasive force measurement reveals the number of active kinesins on a synaptic vesicle precursor regulated by ARL-82018

    • Author(s)
      林 久美子, 長谷川 慎, 佐川 貴志, 田﨑 創平, 丹羽 伸介
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 複数キネシン分子モーターによる協同的なアミロイド前駆タンパク質輸送2018

    • Author(s)
      林 久美子
    • Organizer
      第37回認知症学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Investigation of multiple-dynein transport of melanosomes by non-invasive force measurement using the fluctuation theorem2018

    • Author(s)
      Kumiko Hayashi
    • Organizer
      Asian Biophysics Association Symposium 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Run-time distributions of human KIF1A mutants in hippocampal neurons in relation to hereditary spastic paraplegia2018

    • Author(s)
      Shiori Matsumoto,Kyoko Chiba, Shinsuke Niwa, Kumiko Hayashi
    • Organizer
      Asian Biophysics Association Symposium 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Non-invasive force measurement reveals the number of active kinesins on a synaptic vesicle precursor in axonal transport regulated by ARL-82018

    • Author(s)
      Kumiko Hayashi , Shin Hasegawa, Takashi Sagawa, Sohei Tasaki and Shinsuke Niwa
    • Organizer
      62nd Annual meeting of the Biophysical Society
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 複数分子モーターによる細胞内オルガネラ輸送:モーター数を数える2017

    • Author(s)
      林久美子
    • Organizer
      第69回日本細胞生物学会年会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Non-invasive measurement of force exerted by multiple motor proteins during the axonal transport2017

    • Author(s)
      Kumiko Hayashi
    • Organizer
      19th IUPAB congress and 11th EBSA congress
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Non-invasive force measurement using fluctuation for organelle transport in neurons2017

    • Author(s)
      Kumiko Hayashi
    • Organizer
      2017 Annual meeting of the Australian Society for Biophysics
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ゆらぎを利用した低侵襲な力測定による神経細胞オルガネラ輸送の解明2017

    • Author(s)
      林久美子
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
    • Invited

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Published: 2017-04-28   Modified: 2021-02-19  

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