| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Outline of Final Research Achievements |
Molecular motor kinesin walks along microtubules by alternately moving two motor heads utilizing ATP hydrolysis energy, although the underlying mechanism for the head-head coordination and force production is still unknown. Here we employed single molecule measurements with high temporal and spacial resolutions, and uncovered the underlying mechanism for the preferential detachment of the trailing head before the leading head and for the preferential binding of the unbound head to the forward binding site. In addition, we observed the movement of kinesin under applied loads and elucidated the mechanism how the load decreases its speed.
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