Photosynthetic water oxidation: Molecular mechanism of proton-coupled electron transfer

• Project/Area Number: 17H03662
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Nagoya University
• Principal Investigator: Noguchi Takumi 名古屋大学, 理学研究科, 教授 (60241246)
• Co-Investigator(Kenkyū-buntansha): 杉浦 美羽 愛媛大学, プロテオサイエンスセンター, 准教授 (80312255)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
• Keywords: 生物物理 / 光合成 / 赤外分光 / 水分解 / 電子移動 / 酸素発生 / 光化学系Ⅱ / プロトン移動 / 部位特異的変異 / 赤外分光法 / 量子化学計算

Outline of Final Research Achievements

We studied the molecular mechanism of proton-coupled electron transfer in photosynthetic water oxidation mainly using infrared spectroscopy, quantum chemical calculations, and site-directed mutagenesis. The process of proton and water transfer as well as electron transfer was detected, and proton and water pathways in photosystem II proteins were identified. In addition, the formation process of the Mn4CaO5 cluster, which is a catalytic center of water oxidation, was observed. Furthermore, we found novel mechanism of light-induced amino acid conversation at the ligands of the Mn ions.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果により、天然の光合成における水分解・酸素発生反応の分子機構、およびその触媒部位であるMn4CaO５クラスターの構築過程について多くの知見が得られた。現在人類が直面するエネルギー問題、地球温暖化問題の解決の鍵を握る、人工光合成系の構築および実用化のためには、高効率な水分解触媒の開発が急務であり、本研究で得られた知見は、こうした人工水分解触媒の設計の基本原理を与え、大きく社会に貢献するものであると考えている

Research Products

• [Int'l Joint Research] 中国科学院(中国)
• [Int'l Joint Research] CEA Saclay(フランス)
• [Journal Article] 明らかにされつつある光合成によるエネルギー変換のしくみと応用への期待 (2021)
  • Author(s): 杉浦美羽
  • Journal Title: 電気評論 Volume: 4月号
  • Peer Reviewed
• [Journal Article] Rapid-Scan Time-Resolved ATR-FTIR Study on the Photoassembly of the Water-Oxidizing Mn4CaO5 Cluster in Photosystem II (2021)
  • Author(s): A. Sato, Y. Nakano, S. Nakamura, and T. Noguchi
  • Journal Title: The Journal of Physical Chemistry B Volume: 125 Issue: 16 Pages: 4031-4045
  • DOI: 10.1021/acs.jpcb.1c01624
  • Peer Reviewed
• [Journal Article] A gold nanoparticle conjugate with photosystem I and photosystem II for development of a biohybrid water-splitting photocatalyst (2020)
  • Author(s): Kawahara Kousuke、Inoue-Kahino Natsuko、Namie Keisuke、Kato Yuki、Tomo Tatsuya、Shibata Yutaka、Kashino Yasuhiro、Noguchi Takumi
  • Journal Title: Biomedical Spectroscopy and Imaging Volume: 9 Issue: 1-2 Pages: 73-81
  • DOI: 10.3233/bsi-200200
  • Peer Reviewed / Open Access
• [Journal Article] Molecular Structure of the S2 State with a g = 5 Signal in the Oxygen Evolving Complex of Photosystem II (2020)
  • Author(s): Taguchi Shota、Noguchi Takumi、Mino Hiroyuki
  • Journal Title: The Journal of Physical Chemistry B Volume: 124 Issue: 27 Pages: 5531-5537
  • DOI: 10.1021/acs.jpcb.0c02913
  • NAID: 120006869619
  • Peer Reviewed
• [Journal Article] Formation of the High-Spin S2 State Related to the Extrinsic Proteins in the Oxygen Evolving Complex of Photosystem II (2020)
  • Author(s): Taguchi Shota、Shen Liangliang、Han Guangye、Umena Yasufumi、Shen Jian-Ren、Noguchi Takumi、Mino Hiroyuki
  • Journal Title: The Journal of Physical Chemistry Letters Volume: 11 Issue: 20 Pages: 8908-8913
  • DOI: 10.1021/acs.jpclett.0c02411
  • NAID: 120006890087
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Structural Dynamics of a Protein Domain Relevant to the Water-Oxidizing Complex in Photosystem II as Visualized by High-Speed Atomic Force Microscopy (2020)
  • Author(s): Tokano Takaya、Kato Yuki、Sugiyama Shogo、Uchihashi Takayuki、Noguchi Takumi
  • Journal Title: The Journal of Physical Chemistry B Volume: 124 Issue: 28 Pages: 5847-5857
  • DOI: 10.1021/acs.jpcb.0c03892
  • Peer Reviewed
• [Journal Article] Protonation structure of the photosynthetic water oxidizing complex in the S0 state as revealed by normal mode analysis using quantum mechanics/molecular mechanics calculations (2020)
  • Author(s): Yamamoto Masao、Nakamura Shin、Noguchi Takumi
  • Journal Title: Physical Chemistry Chemical Physics Volume: 22 Issue: 42 Pages: 24213-24225
  • DOI: 10.1039/d0cp04079g
  • Peer Reviewed
• [Journal Article] Protonation State of a Key Histidine Ligand in the Iron-Quinone Complex of Photosystem II as Revealed by Light-Induced ATR-FTIR Spectroscopy (2020)
  • Author(s): Kimura Masakazu、Kato Yuki、Noguchi Takumi
  • Journal Title: Biochemistry Volume: 59 Issue: 45 Pages: 4336-4343
  • DOI: 10.1021/acs.biochem.0c00810
  • Peer Reviewed
• [Journal Article] Probing the role of Arginine 323 of the D1 protein in Photosystem II function (2020)
  • Author(s): Sugiura, M., Taniguchi, T., Tango, N., Nakamura, M., Selles, J., and Boussac, A.
  • Journal Title: Physiol. Plant. Volume: - Issue: 2 Pages: 183-199
  • DOI: 10.1111/ppl.13115
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Fourier transform infrared and mass spectrometry analyses of a site-directed mutant of D1-Asp170 as a ligand to the water-oxidizing Mn4CaO5 cluster in photosystem II (2020)
  • Author(s): T. Kitajima-Ihara, T. Suzuki, S. Nakamura, Y. Shimada, R. Nagao, N. Dohmae, and T. Noguchi
  • Journal Title: Biochim. Biophys. Acta Bioenergetics Volume: 1861 Issue: 1 Pages: 148086-148086
  • DOI: 10.1016/j.bbabio.2019.148086
  • Peer Reviewed
• [Journal Article] Role of the O4 channel in photosynthetic water oxidation as revealed by Fourier transform infrared difference and time-resolved infrared analysis of the D1-S169A mutant (2020)
  • Author(s): Y. Shimada, T. Kitajima-Ihara, R. Nagao, and T. Noguchi
  • Journal Title: J. Phys. Chem. B Volume: 124 Issue: 8 Pages: 1470-1480
  • DOI: 10.1021/acs.jpcb.9b11946
  • Peer Reviewed
• [Journal Article] What can we still learn from the electrochromic band-shifts in Photosystem II? (2020)
  • Author(s): A. Boussac, J. Selles, and M. Sugiura,
  • Journal Title: Biochim. Biophys. Acta Bioenergetics Volume: 1861 Issue: 5-6 Pages: 148176-148176
  • DOI: 10.1016/j.bbabio.2020.148176
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Proton Release Process during the S2-to-S3 Transition of Photosynthetic Water Oxidation As Revealed by the pH Dependence of Kinetics Monitored by Time-Resolved Infrared Spectroscopy (2019)
  • Author(s): Takemoto Hiroshi、Sugiura Miwa、Noguchi Takumi
  • Journal Title: Biochemistry Volume: 58 Issue: 42 Pages: 4276-4283
  • DOI: 10.1021/acs.biochem.9b00680
  • Peer Reviewed
• [Journal Article] FTIR Microspectroscopic Analysis of the Water Oxidation Reaction in a Single Photosystem II Microcrystal (2019)
  • Author(s): Kato Yuki、Haniu Satoshi、Nakajima Yoshiki、Akita Fusamichi、Shen Jian-Ren、Noguchi Takumi
  • Journal Title: The Journal of Physical Chemistry B Volume: 124 Issue: 1 Pages: 121-127
  • DOI: 10.1021/acs.jpcb.9b10154
  • Peer Reviewed / Open Access
• [Journal Article] Does the water-oxidizing Mn4CaO5 cluster regulate the redox potential of the primary quinone electron acceptor QA in photosystem II? A study by Fourier transform infrared spectroelectrochemistry (2019)
  • Author(s): Kato Yuki、Ohira Ayaka、Nagao Ryo、Noguchi Takumi
  • Journal Title: Biochimica et Biophysica Acta (BBA) - Bioenergetics Volume: 1860 Issue: 12 Pages: 148082-148082
  • DOI: 10.1016/j.bbabio.2019.148082
  • Peer Reviewed
• [Journal Article] An alternative plant-like cyanobacterial ferredoxin with unprecedented structural and functional properties (2019)
  • Author(s): Motomura Taiki、Zuccarello Lidia、S?tif Pierre、Boussac Alain、Umena Yasufumi、Lemaire David、Tripathy Jatindra N.、Sugiura Miwa、Hienerwadel Rainer、Shen Jian-Ren、Berthomieu Catherine
  • Journal Title: Biochimica et Biophysica Acta (BBA) - Bioenergetics Volume: 1860 Issue: 11 Pages: 148084-148084
  • DOI: 10.1016/j.bbabio.2019.148084
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Redox-state dependent blinking of single photosystem I trimers at around liquid-nitrogen temperature (2019)
  • Author(s): Jana, S., Du, T., Nagao, R., Noguchi, T., Shibata, Y.
  • Journal Title: Biochim. Biophys. Acta, Bioenerg. Volume: 1860 Issue: 1 Pages: 30-40
  • DOI: 10.1016/j.bbabio.2018.11.002
  • Peer Reviewed
• [Journal Article] New insights on ChlD1 function in Photosystem II from site-directed mutants of D1-T179 in Thermosynechococcus elongatus (2019)
  • Author(s): Takegawa, Y., Nakamura, M., Nakamura, S., Noguchi, T., Selles, J., Rutherford, A. W., Boussac, A., and Sugiura, M.
  • Journal Title: Biochim. Biophys. Acta (Bioenergetics) Volume: 1860 Issue: 4 Pages: 297-309
  • DOI: 10.1016/j.bbabio.2019.01.008
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Initial Mn2+ binding site in photoassembly of the water-oxidizing Mn4CaO5 cluster in photosystem II as studied by quantum mechanics/molecular mechanics calculations (2019)
  • Author(s): S. Nakamura and T. Noguchi
  • Journal Title: Chem. Phys. Lett. Volume: 721 Pages: 62-67
  • DOI: 10.1016/j.cplett.2019.02.029
  • Peer Reviewed
• [Journal Article] Fourier Transform Infrared Analysis of the S-State Cycle of Water Oxidation in the Microcrystals of Photosystem II (2018)
  • Author(s): Kato Yuki、Akita Fusamichi、Nakajima Yoshiki、Suga Michihiro、Umena Yasufumi、Shen Jian-Ren、Noguchi Takumi
  • Journal Title: The Journal of Physical Chemistry Letters Volume: 9 Issue: 9 Pages: 2121-2126
  • DOI: 10.1021/acs.jpclett.8b00638
  • Peer Reviewed / Open Access
• [Journal Article] pH-Dependent regulation of the relaxation rate of the radical anion of the secondary quinone electron acceptor QB in photosystem II as revealed by Fourier transform infrared spectroscopy (2018)
  • Author(s): Y. Nozawa, and T. Noguchi
  • Journal Title: Biochemistry Volume: 57 Issue: 19 Pages: 2828-2836
  • DOI: 10.1021/acs.biochem.8b00263
  • Peer Reviewed
• [Journal Article] Mechanism of methanol inhibition of photosynthetic water oxidation as studied by Fourier transform infrared difference and time-resolved infrared spectroscopies (2018)
  • Author(s): H. Yata, and T. Noguchi
  • Journal Title: Biochemistry Volume: 57 Issue: 32 Pages: 4803-4815
  • DOI: 10.1021/acs.biochem.8b00596
  • Peer Reviewed
• [Journal Article] Thylakoid membrane lipid sulfoquinovosyl-diacylglycerol (SQDG) is required for full functioning of photosystem II in Thermosynechococcus elongatus (2018)
  • Author(s): Nakajima, Y., Umena, Y., Nagao, R., Endo, K., Kobayashi, K., Akita, F., Suga, M., Wada, H., Noguchi, T., Shen, J.-R.
  • Journal Title: J. Biol. Chem. Volume: 293 Issue: 38 Pages: 14786-14797
  • DOI: 10.1074/jbc.ra118.004304
  • Peer Reviewed / Open Access
• [Journal Article] Mechanism of proton-coupled electron transfer in the S0-to-S1 transition of photosynthetic water oxidation as revealed by time-resolved infrared spectroscopy (2018)
  • Author(s): T. Shimizu, M. Sugiura, and T. Noguchi
  • Journal Title: J. Phys. Chem. B. Volume: 122 Issue: 41 Pages: 9460-9470
  • DOI: 10.1021/acs.jpcb.8b07455
  • Peer Reviewed
• [Journal Article] Evaluation of photosynthetic activities in thylakoid membranes by means of Fourier transform infrared spectroscopy (2018)
  • Author(s): Nagao, R., Kitazaki, S., Noguchi, T.
  • Journal Title: Biochim. Biophys. Acta, Bioenerg. Volume: 1859 Issue: 2 Pages: 129-136
  • DOI: 10.1016/j.bbabio.2017.11.004
  • Peer Reviewed
• [Journal Article] PsbP-induced protein conformational changes around Cl? ions in the water oxidizing center of photosystem II (2018)
  • Author(s): J. Kondo, and T. Noguchi
  • Journal Title: Photosynthetica Volume: 56 Issue: SPECIAL ISSUE Pages: 178-184
  • DOI: 10.1007/s11099-017-0749-5
  • Peer Reviewed
• [Journal Article] Fluorescence property of photosystem II protein complexes bound to a gold nanoparticle (2017)
  • Author(s): K. Tahara, A. Mohamed, K. Kawahara, R. Nagao, Y. Kato, H. Fukumura, Y. Shibata, T. Noguchi
  • Journal Title: Faraday Discussions Volume: 印刷中 Pages: 121-134
  • DOI: 10.1039/c6fd00188b
  • NAID: 120006343676
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Light-induced structural changes and the site of O=O bond formation in PSII caught by XFEL. (2017)
  • Author(s): Suga, M.†, Akita, F.†, Sugahara, M.†, Kubo, M.†, Nakajima, Y.† et al. (†equal contribution)(38人中1番目)
  • Journal Title: Nature Volume: 543 Issue: 7643 Pages: 131-135
  • DOI: 10.1038/nature21400
  • NAID: 120006344935
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Monitoring the reaction process during the S2→S3 transition in photosynthetic water oxidation using time-resolve infrared spectroscopy (2017)
  • Author(s): H. Sakamoto, T. Shimizu, R. Nagao, T. Noguchi
  • Journal Title: J. Am. Chem. Soc. Volume: 139 Issue: 5 Pages: 2022-2029
  • DOI: 10.1021/jacs.6b11989
  • NAID: 120006305572
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genetically introduced hydrogen bond interactions reveal an asymmetric charge distribution on the radical cation of the special-pair chlorophyll P680 (2017)
  • Author(s): R. Nagao, M. Yamaguchi, S. Nakamura, H. Ueoka-Nakanishi, T. Noguchi
  • Journal Title: J. Biol. Chem. Volume: 印刷中 Issue: 18 Pages: 7474-7486
  • DOI: 10.1074/jbc.m117.781062
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Infrared determination of the protonation state of a key histidine residue in the photosynthetic water oxidizing center (2017)
  • Author(s): S. Nakamura, T. Noguchi
  • Journal Title: J. Am. Chem. Soc. Volume: 139 Issue: 27 Pages: 9364-9375
  • DOI: 10.1021/jacs.7b04924
  • Peer Reviewed
• [Journal Article] D1-Asn-298 in photosystem II is involved in a hydrogen-bond network near the redox-active tyrosine YZ for proton exit during water oxidation (2017)
  • Author(s): Nagao, R., Ueoka-Nakanishi, H., Noguchi, T.
  • Journal Title: J. Biol. Chem. Volume: 292 Issue: 49 Pages: 20046-20057
  • DOI: 10.1074/jbc.m117.815183
  • NAID: 120006473518
  • Peer Reviewed
• [Presentation] 光化学系Ⅱにおけるルーメン側タンパク質ドメインの動的構造変化の高速AFM観察 (2021)
  • Author(s): 戸叶貴也、加藤祐樹、杉山翔吾、内橋貴之、野口巧
  • Organizer: 新学術領域研究「革新的光物質変換」第3回公開シンポジウム
  • Invited
• [Presentation] Effects of herbicide and formate on the redox potential of the primary quinone QA in photosystem II (2021)
  • Author(s): 加藤祐樹、野口巧
  • Organizer: 日本植物生理学会年会
• [Presentation] Mechanism of novel amino acid conversion of a Mn-cluster ligand in photosystem II (2021)
  • Author(s): 嶋田友一郎、鈴木健裕、北島(井原)智美、長尾遼、堂前直、野口巧
  • Organizer: 日本植物生理学会年会
• [Presentation] Formation of high spin S2 intermediate state related to g~5 EPR signal in the oxygen evolving complex (2021)
  • Author(s): 三野広幸、田口翔太、Liangliang Shen、Guangye Han、梅名泰史、沈建仁、野口巧
  • Organizer: 日本植物生理学会年会
• [Presentation] Effects of mutations in the Loop4 region of PsbP on the oxygen-evolving activity of photosystem II (2021)
  • Author(s): 今泉滉、西村大志、長尾遼、加藤祐樹、中野雄司、野口巧、伊福健太郎
  • Organizer: 日本植物生理学会年会
• [Presentation] 光化学系IIにおける励起・電荷分離の分子機構 (2021)
  • Author(s): 杉浦美羽
  • Organizer: 新学術領域研究「革新的光物質変換」第3回公開シンポジウム
• [Presentation] FTIR spectroelectrochemical study on the mechanism of the pH dependence of the redox potential of the non-heme iron in photosystem II (2020)
  • Author(s): Yuki Kato, Hiroki Watanabe, Takumi Noguchi
  • Organizer: 日本生物物理学会年会
• [Presentation] Time-resolved infrared detection of electron transfer between quinone electron acceptors QA and QB in photosystem II (2020)
  • Author(s): 伊藤帆奈美、加藤佑樹、野口巧
  • Organizer: 日本生物物理学会年会
• [Presentation] Role of D1-His252 in the reaction of the secondary quinone electron accepter QB in photosystem II (2020)
  • Author(s): 小林智幸、嶋田友一郎、長尾遼、野口巧
  • Organizer: 日本生物物理学会年会
• [Presentation] Novel amino acid conversion of a His mutant of D1-Asp170 in photosystem II (2020)
  • Author(s): 嶋田友一郎、北島(井原)智美、長尾遼、野口巧
  • Organizer: 日本生物物理学会年会
• [Presentation] Molecular structure of the S2 state with a g = 5 signal in the oxygen evolving complex of photosystem II (2020)
  • Author(s): Hiroyuki Mino、Shota Taguchi、Takumi Noguchi
  • Organizer: 日本生物物理学会年会
• [Presentation] Involvement of the chloride channel in proton transfer during water oxidation in photosystem II (2020)
  • Author(s): 杉山綾音、嶋田友一郎、長尾遼、野口巧
  • Organizer: 日本生物物理学会年会
• [Presentation] g = 5 をもつ酸素発生系マンガンクラスターの分子構造 (2020)
  • Author(s): 三野広幸、田口翔太、野口巧
  • Organizer: 電子スピンサイエンス学会年会
• [Presentation] 高速原子間力顕微鏡を用いた光化学系IIの動態観察 (2020)
  • Author(s): 戸叶貴也、加藤祐樹、杉山翔吾、野口巧、内橋貴之
  • Organizer: 日本生体エネルギー研究会
• [Presentation] 顕微赤外分光法を用いた光合成光化学系IIの単一微結晶における水分解反応の解析 (2020)
  • Author(s): 加藤祐樹, 埴生悟史, 中島芳樹, 秋田総理, 沈 建仁, 野口 巧
  • Organizer: 日本物理学会年会
• [Presentation] 光化学系IIのpHに依存した電子移動制御におけるD1-H252の役割 (2020)
  • Author(s): 嶋田友一郎、中島聖竜、小林智幸、長尾遼、野口巧
  • Organizer: 日本植物生理学会年会
• [Presentation] 光化学系IIにおけるMnクラスターのカルボキシレート配位子変異体の光誘起アミノ酸変換 (2020)
  • Author(s): 北島(井原)智美、鈴木健裕、中村伸、嶋田友一郎、長尾遼、堂前直、野口巧
  • Organizer: 日本植物生理学会年会
• [Presentation] Infrared analyses of electron and proton transfer reactions in photosystem II (2019)
  • Author(s): Takumi Noguchi
  • Organizer: Annual Meeting of the French Photosynthesis Society,
  • Int'l Joint Research / Invited
• [Presentation] 顕微赤外分光法による光化学系IIの単一微結晶における水分解反応の解析 (2019)
  • Author(s): 加藤祐樹、埴生悟史、中島芳樹、秋田総理、沈 建仁、野口 巧
  • Organizer: 第10回日本光合成学会年会
• [Presentation] 光化学系IIにおけるpHに依存する電子移動制御の分子機構、第10回日本光合成学会年会 (2019)
  • Author(s): 嶋田友一郎、中島聖竜、長尾遼、野口巧
  • Organizer: 第10回日本光合成学会年会
• [Presentation] FTIR study on the water oxidation reaction in photosystem II microcrystals (2019)
  • Author(s): Yuki Kato, Fusamichi Akita, Yoshiki Nakajima, Satoshi Haniu, Michihiro Suga, Yasufumi Umena, Jian-Ren Shen, Takumi Noguchi
  • Organizer: 10th International Conference Photosynthesis and Hydrogen Energy Research for Sustainability-2019
  • Int'l Joint Research
• [Presentation] Infrared microspectroscopic study on water oxidation in a single photosystem II microcrystal (2019)
  • Author(s): Yuki Kato, Satoshi Haniu, Yoshiki Nakajima, Fusamichi Akita, Jian-Ren Shen, Takumi Noguchi
  • Organizer: 日本生物物理学会年会
• [Presentation] Time-resolved infrared analysis of proton release pathways in photosynthetic water oxidation using a D1-N298A mutant and NO3- substitution (2019)
  • Author(s): Yasutada Okamoto, Yuichiro Shimada, Ryo Nagao, Takumi Noguchi
  • Organizer: 日本生物物理学会年会
• [Presentation] QM/MM analysis of the protonation structure of the S0 state in the water-oxidizing Mn4CaO5 cluster (2019)
  • Author(s): Masao Yamamoto, Shin Nakamura, and Takumi Noguchi
  • Organizer: 日本生物物理学会年会
• [Presentation] Equilibrium of the S2-state isomers of the water-oxidizing Mn4CaO5 cluster in photosystem II regulated by extrinsic proteins (2019)
  • Author(s): Shota Taguchi, Liangliang Shen, Guangye Han, Jian-Ren Shen, Takumi Noguchi, Hiroyuki Mino
  • Organizer: 日本生物物理学会年会
• [Presentation] FTIR study on the localization of the excited triplet state of chlorophyll in photosystem II (2019)
  • Author(s): Taichi Hayase, Yuichiro Shimada, Ryo Nagao, and Takumi Noguchi
  • Organizer: 日本生物物理学会年会
• [Presentation] Molecular mechanism of pH-dependent electron-flow regulation in photosystem II (2019)
  • Author(s): Yuichio Shimada, Seiryu Nakajima, Ryo Nagao, Takumi Noguchi
  • Organizer: 日本生物物理学会年会
• [Presentation] Dynamics of photosystem II protein complexes as observed by high speed atomic force microscopy (2019)
  • Author(s): Takaya Tokano, Yuki Kato, Syogo Sugiyama, Takumi Noguchi, Takayuki Uchihashi
  • Organizer: 日本生物物理学会年会
• [Presentation] Infrared detection of protons and water molecules in photosynthetic water oxidation (2019)
  • Author(s): Takumi Noguchi
  • Organizer: 日本生物物理学会年会
  • Invited
• [Presentation] FTIR microspectroscopic study on the water oxidation in a single photosystem II microcrystal (2019)
  • Author(s): Yuki Kato, Satoshi Haniu, Yoshiki Nakajima, Fusamichi Akita, Jian-Ren Shen, and Takumi Noguchi
  • Organizer: 3rd International Solar Fuels Conference/International Conference on Artificial Photosynthesis-2019
  • Int'l Joint Research
• [Presentation] Infrared studies on the water oxidation and electron transfer reactions in photosystem II (2019)
  • Author(s): Takumi Noguchi
  • Organizer: 3rd International Solar Fuels Conference/International Conference on Artificial Photosynthesis-2019
  • Int'l Joint Research / Invited
• [Presentation] Single molecule spectroscopy on photosystem i revealed the dynamical nature of the light harvesting (2019)
  • Author(s): Yutaka Shibata, Sankar Jana, Ting Du, Ryo Nagao, and Takumi Noguchi
  • Organizer: 3rd International Solar Fuels Conference/International Conference on Artificial Photosynthesis-2019
  • Int'l Joint Research
• [Presentation] 光化学系IIアクセサリークロロフィルの軸配位子環境と強光との関係 (2019)
  • Author(s): 木村仁哉、中村誠、竹川裕紀、杉浦美羽
  • Organizer: 第10回日本光合成学会年会
• [Presentation] ChlD1の異なるリガンド構造がP680の機能にもたらす影響 (2019)
  • Author(s): 島本直拓、中村誠、竹川裕紀、杉浦美羽
  • Organizer: 第10回日本光合成学会年会
• [Presentation] The role of Valine 185 of the D1 protein in the Photosystem II oxygen evolution (2019)
  • Author(s): Miwa Sugiura, Itsuki Takachi, Yuya Hara, Shin Kanawaku, Julien Selles, and Alain Boussac
  • Organizer: 3rd International Solar Fuels Conference/International Conference on Artificial Photosynthesis-2019
  • Int'l Joint Research
• [Presentation] X-Ray crystallographic analysis of Photosystem II from a PsbA2-only strain and its complex with bromoxynil (2019)
  • Author(s): Naoki Tone, Natsumi Ugai, Yoshiki Nakajima, Michihiro Suga, Fusamichi Akita, Yasufumi Umena, Akiko Nakagawa, Miwa Sugiura, Jian-Ren Shen
  • Organizer: 3rd International Solar Fuels Conference/International Conference on Artificial Photosynthesis-2019
  • Int'l Joint Research
• [Presentation] 光化学系IIのD1/Val185は水の酸化反応過程で多機能な役割を担う (2019)
  • Author(s): 杉浦美羽
  • Organizer: 第17回JST-さきがけ領域会議
• [Presentation] 光化学系Ⅱにおける電子・プロトン移動反応の赤外分光解析 (2019)
  • Author(s): 野口 巧
  • Organizer: 水和ナノ構造研究会
  • Invited
• [Presentation] Infrared analyses of photoreactions in photosystem II (2019)
  • Author(s): Takumi Noguchi
  • Organizer: 10th OCARINA International Symposium
  • Int'l Joint Research / Invited
• [Presentation] 光化学系II微結晶の水分解系における中間状態遷移の赤外分光解析 (2019)
  • Author(s): 加藤 祐樹、埴生 悟史、秋田 総理、中島 芳樹、菅 倫寛、梅名 泰史、沈 建仁、野口 巧
  • Organizer: 日本物理学会
• [Presentation] 光化学系IIの単一微結晶における水分解反応の顕微赤外分光解析 (2019)
  • Author(s): 加藤 祐樹、埴生 悟史、中島 芳樹、秋田 総理、沈 建仁、野口 巧
  • Organizer: 日本植物生理学会年会
• [Presentation] 光化学系IIのD1-S169A変異体を用いた水分解機構の解析 (2019)
  • Author(s): 嶋田 友一郎、北島（井原） 智美、長尾 遼、野口 巧
  • Organizer: 日本植物生理学会年会
• [Presentation] 光化学系Ⅱにおける水分解Mn4CaO5クラスターのS2構造異性体平衡の種依存性の要因 (2019)
  • Author(s): 田口 翔太、Shen Liangliang、Han Guangye、沈 建仁、野口 巧、三野 広幸
  • Organizer: 日本植物生理学会年会
• [Presentation] FTIR分光電気化学法による光化学系II第一キノン電子受容体QAの酸化還元特性の解明 (2019)
  • Author(s): 加藤 祐樹、大平 彩花、長尾 遼,、野口 巧
  • Organizer: 生物物理学会中部支部講演会
• [Presentation] 光化学系IIのD1-S169A変異体を用いた光合成水分解機構の赤外分光解析 (2019)
  • Author(s): 嶋田 友一郎、北島(井原) 智美、長尾 遼、野口 巧
  • Organizer: 生物物理学会中部支部講演会
• [Presentation] 高速原子間力顕微鏡を用いた光化学系IIの観察 (2019)
  • Author(s): 戸叶 貴也、加藤 祐樹、杉山 翔吾、野口 巧、内橋 貴之
  • Organizer: 生物物理学会中部支部講演会
• [Presentation] 光合成光化学系IIにおける第一キノン電子受容体QAの酸化還元特性 (2019)
  • Author(s): 加藤 祐樹、大平 彩花、長尾 遼、野口 巧
  • Organizer: 電気化学会第86回大会
• [Presentation] New Insights on ChlD1 Function in PhotosystemⅡ from Site-Directed Mutants (2019)
  • Author(s): M. Sugiura
  • Organizer: 10th OCARINA International Symposium
  • Int'l Joint Research / Invited
• [Presentation] 光化学系IIにおける非ヘム鉄の酸化還元電位のpH依存性：FTIR分光電気化学法を用いた解析 (2018)
  • Author(s): 渡邊 大貴、野口 巧、加藤 祐樹
  • Organizer: 日本光合成学会年会およびシンポジウム
• [Presentation] 光合成水分解反応のS2→S3遷移におけるプロトン共役電子移動の時間分解赤外分光解析 (2018)
  • Author(s): 竹本 寛、野口 巧
  • Organizer: 日本光合成学会年会およびシンポジウム
• [Presentation] 光化学系IIにおけるMn4CaO5クラスター近傍に位置する D1-Ser169の水分解反応への関与 (2018)
  • Author(s): 嶋田 友一郎、北島 智美、長尾 遼、野口 巧
  • Organizer: 日本光合成学会年会およびシンポジウム
• [Presentation] FTIR分光法で明らかになった光化学系II第一キノン電子受容体QAの酸化還元特性 (2018)
  • Author(s): 加藤 祐樹、大平 彩花、長尾 遼、野口 巧
  • Organizer: 生体分子科学討論会
• [Presentation] 光化学系II非ヘム鉄の酸化還元電位のpH依存性 (2018)
  • Author(s): 渡邊 大貴、野口 巧、加藤 祐樹
  • Organizer: 第26回光合成セミナー
• [Presentation] 光化学系Ｉの単一分子分光 (2018)
  • Author(s): 柴田 穣、小林 誉宗、Jana Sankar、長尾 遼、野口 巧
  • Organizer: 第26回光合成セミナー
• [Presentation] 単一分子励起スペクトル測定による光化学系Ⅰの光捕集過程の研究 (2018)
  • Author(s): 小林 誉宗、Jana Sankar、長尾 遼、野口 巧、柴田 穣
  • Organizer: 第12回分子科学討論会
• [Presentation] Regulation mechanism of electron and proton transfer reactions in photosystem II (2018)
  • Author(s): Takumi Noguchi
  • Organizer: 1st Asia-Oceania International Congress on Photosynthesis
  • Int'l Joint Research / Invited
• [Presentation] Infrared analyses of proton-coupled electron transfer in photosynthetic light-energy conversion (2018)
  • Author(s): Takumi Noguchi
  • Organizer: 日本生物物理学会年会
  • Invited
• [Presentation] FTIR study on the S-state cycle of water oxidation in the microcrystals of photosystem II (2018)
  • Author(s): Yuki Kato, Fusamichi Akita, Yoshiki Nakajima, Michihiro Suga, Yasufumi Umena, Jian-Ren Shen, Takumi Noguchi
  • Organizer: 日本生物物理学会年会
• [Presentation] Effect of replacement of Cl- with NO3- on photosynthetic water oxidation as studied by time-resolved infrared spectroscopy (2018)
  • Author(s): Yasutada Okamoto, Takumi Noguchi
  • Organizer: 日本生物物理学会年会
• [Presentation] QM/MM analysis of the DOD vibrations of water molecules around the Mn4CaO5 cluster in photosystem II (2018)
  • Author(s): Masao Yamamoto, Shin Nakamura, Takumi Noguchi
  • Organizer: 日本生物物理学会年会
• [Presentation] Mechanism of proton-coupled electron transfer in the S2→S3 transition of photosynthetic water oxidation revealed by TRIR analysis (2018)
  • Author(s): Hiroshi Takemoto, Takumi Noguchi
  • Organizer: 日本生物物理学会年会
• [Presentation] FTIR-spectroelectrochemical study on the pH dependence of the redox potential of the non-heme iron in photosystem II (2018)
  • Author(s): Hiroki Watanabe, Takumi Noguchi, Yuki Kato
  • Organizer: 日本生物物理学会年会
• [Presentation] Role of D1-Ser169 near O4 of the Mn4CaO5 cluster in photosynthetic water oxidation (2018)
  • Author(s): Yuichio Shimada, Tomomi Kitajima-Ihara, Ryo Nagao, Takumi Noguchi
  • Organizer: 日本生物物理学会年会
• [Presentation] Molecular Mechanisms of Efficient Electron Transfer and Water Oxidation in Photosystem II (2018)
  • Author(s): M. Sugiura
  • Organizer: 1st Evolutionary Materials Workshop in Seoul National University
  • Int'l Joint Research / Invited
• [Presentation] 光合成による高効率エネルギー変換機構の理解と応用研究はどこまで進んだか？ (2018)
  • Author(s): 杉浦 美羽
  • Organizer: 平成30年度日本農芸化学会西日本支部大会シンポジウム「限りある資源、地球環境への農芸化学からの取り組み」
  • Invited
• [Presentation] 光化学系IIの水の酸化反応におけるD1/V185の役割について (2018)
  • Author(s): 高智 五輝、原 侑也、Alain Boussac、杉浦 美羽
  • Organizer: 第56回日本生物物理学会年会
• [Presentation] Role of accessory chlorophyll ChlD1 as P680 in Photosystem II (2018)
  • Author(s): M. Sugiura
  • Organizer: 1st Asia-Oceania International Congress on Photosynthesis
  • Int'l Joint Research / Invited
• [Presentation] 光化学系IIの水の酸化反応におけるD1-V185の役割の解明 (2018)
  • Author(s): 高智 五輝、原 侑也、Alain Boussac、杉浦 美羽
  • Organizer: 第9回日本光合成学会年会
• [Presentation] 光化学系IIを構成するCyb559のヘム周辺構造の変化がアクセプター側へ及ぼす影響 (2018)
  • Author(s): 中村 誠、Alain Boussac、杉浦 美羽
  • Organizer: 第9回日本光合成学会年会
• [Presentation] 光合成水分解マンガンクラスターの酸化還元電位の制御機構 (2018)
  • Author(s): 野口 巧、中村 伸
  • Organizer: 新学術領域研究「革新的光物質変換」第1回公開シンポジウム
• [Presentation] 光化学系IIにおけるMnクラスターの光活性化過程：FTIRおよび量子化学計算による解析 (2018)
  • Author(s): 中村 伸、佐藤 彰彦、野口 巧
  • Organizer: 日本生物物理学会中部支部講演会
• [Presentation] 天然光合成における電子移動制御の分子機構 (2018)
  • Author(s): 野口 巧
  • Organizer: 日本化学会第98春季年会
  • Invited
• [Presentation] FTIR分光法による光化学系IIにおける第一キノン電子受容体QAの酸化還元特性の解明 (2018)
  • Author(s): 加藤 祐樹、大平 彩花、長尾 遼、野口 巧
  • Organizer: 日本物理学会第73回年次大会
• [Presentation] 光化学系Iの極低温単一分子分光 (2018)
  • Author(s): 小林 誉宗、Jana Sankar、杜 亭、長尾 遼、野口 巧、柴田 穣
  • Organizer: 日本植物生理学会年会
• [Presentation] FTIR分光電気化学法による光化学系II第一キノンQAの酸化還元電位計測：Mn除去の効果 (2017)
  • Author(s): 大平 彩花、長尾 遼、野口 巧、加藤 祐樹
  • Organizer: 日本光合成学会年会
• [Presentation] 光合成水分解反応の赤外分光解析 (2017)
  • Author(s): 中村 伸、野口 巧
  • Organizer: 日本光合成学会年会
  • Invited
• [Presentation] 光化学系II結晶における水分解反応のATR-FTIR解析 (2017)
  • Author(s): 加藤 祐樹、秋田 総理、中島 芳樹、菅 倫寛、梅名 泰史、沈 建仁、野口 巧
  • Organizer: 生体分子科学討論会
• [Presentation] 光合成水分解系におけるメタノール阻害機構のフーリエ変換赤外分光解析 (2017)
  • Author(s): 矢田 はる奈、野口 巧
  • Organizer: 生体分子科学討論会
• [Presentation] 光化学系IIにおいてMn除去してもQAの酸化還元電位は変動しない (2017)
  • Author(s): 大平 彩花、長尾 遼、野口 巧、加藤 祐樹
  • Organizer: 第25回光合成セミナー2017
• [Presentation] Development of novel scanning microscope for excitation spectra measurement (2017)
  • Author(s): Sankar Jana, Takanori Kobayashi, Ryo Nagao, Takumi Noguchi, Yutaka Shibata
  • Organizer: 光化学討論会
• [Presentation] 光化学系Iタンパク質複合体の単一分子蛍光分光 (2017)
  • Author(s): 柴田 穣、小林 誉宗、杜 亭、ジャナ サンカー、長尾 遼、野口 巧
  • Organizer: 分子化学討論会
• [Presentation] ATR-FTIR analysis of the S-state transitions during water oxidation in photosystem II crystals (2017)
  • Author(s): Yuki Kato, Fusamichi Akita, Yoshiki Nakajima, Michihiro Suga, Yasufumi Umena, Jian-Ren Shen, Takumi Noguchi
  • Organizer: 日本生物物理学会年会
• [Presentation] Influence of Mn depletion on the redox potential of the primary quinone QA in photosystem II as revealed by FTIR spectroelectrochemistry (2017)
  • Author(s): Ayaka Ohira, Ryo Nagao, Takumi Noguchi, Yuki Kato
  • Organizer: 日本生物物理学会年会
• [Presentation] FTIR and quantum chemical calculation study of the photoactivation process of the Mn cluster in photosystem II (2017)
  • Author(s): Shin Nakamura, Akihiko Sato, Takumi Noguchi
  • Organizer: 日本生物物理学会年会
• [Presentation] ATR-FTIR study on the protonation state of a histidine ligand to the non-heme iron in photosystem II (2017)
  • Author(s): Masakazu Kimura, Shin Nakamura, Yuki Kato, Takumi Noguchi
  • Organizer: 日本生物物理学会年会
• [Presentation] Single-molecule spectroscopy of photosystem I at low temperature: The origin of the blinking (2017)
  • Author(s): Yutaka Shibata, Takanori Kobayashi, Ting Du, Sankar Jana, Ryo Nagao, Takumi Noguchi
  • Organizer: 日本生物物理学会年会
• [Presentation] FTIR study on the redox property of the primary quinone QA in photosystem II (2017)
  • Author(s): Yuki Kato, Ayaka Ohira, Ryo Nagao, and Takumi Noguchi
  • Organizer: 8th International Conference “Photosynthesis and Hydrogen Energy Research for Sustainability ? 2017”
  • Int'l Joint Research / Invited
• [Presentation] Single-molecule spectroscopy study on photosystem I at low temperatures (2017)
  • Author(s): Yutaka Shibata, Sankar Jana, Takanori Kobayashi, Ting Du, Ryo Nagao, and Takumi Noguchi
  • Organizer: 8th International Conference “Photosynthesis and Hydrogen Energy Research for Sustainability ? 2017”
  • Int'l Joint Research / Invited
• [Presentation] Mechanism of proton?coupled electron transfer in the S2→S3 transition of photosynthetic water oxidation revealed by time-resolved infrared analysis (2017)
  • Author(s): Hiroshi Takemoto, and Takumi Noguchi
  • Organizer: IGER International Symposium on Science of Molecular Assembly and Biomolecular Systems 2017
  • Int'l Joint Research
• [Presentation] Protonation structure of a key His residue interacting with the Mn cluster in photosystem II as revealed by polarized ATR-FTIR spectroscopy (2017)
  • Author(s): Shin Nakamura and Takumi Noguchi
  • Organizer: IGER International Symposium on Science of Molecular Assembly and Biomolecular Systems 2017
  • Int'l Joint Research
• [Presentation] Infrared analysis of electron transfer and water oxidation reactions in Photosystem II (2017)
  • Author(s): Takumi Noguchi
  • Organizer: Japan-France Joint Workshop on the Structure and Function of Photosystem
  • Int'l Joint Research / Invited
• [Book] 量子力学／分子力学（QM/MM）計算による金属タンパク質の研究、生命金属ダイナミクス－生体内における金属の挙動と制御 (2021)
  • Author(s): 中村伸、野口巧、吉澤一成
  • Total Pages: 10
  • Publisher: エヌ・ティー・エス
  • ISBN: 9784860437060
• [Book] Molecular mechanism of asymmetric electron transfer on the electron donor side of photosystem II, Photosynthesis: Molecular Approaches to Solar Energy Conversion (2021)
  • Author(s): Takumi Noguchi
  • Total Pages: 17
  • Publisher: Springer
  • ISBN: 9783030674069
• [Remarks] Nagoya Phys G lab
• [Remarks] Nagoya Phys G lab
  • URL: https://www.bio.phys.nagoya-u.ac.jp/
• [Remarks] 名古屋大学理学研究科光生体エネルギー研究室ホームページ
  • URL: https://www.bio.phys.nagoya-u.ac.jp/