Epigenetic modification of sperm and its trans-generational changes.

Research Project

Project/Area Number	17H03680
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Research Field	Developmental biology
Research Institution	University of Yamanashi
Principal Investigator	KOHDA Takashi 山梨大学, 大学院総合研究部, 教授 (60211893)
Co-Investigator(Kenkyū-buntansha)	川崎佑季東京医科歯科大学, 難治疾患研究所, 助教 (60746890)
Project Period (FY)	2017-04-01 – 2020-03-31
Project Status	Completed (Fiscal Year 2019)
Budget Amount *help	¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000) Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000) Fiscal Year 2018: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000) Fiscal Year 2017: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Keywords	ヒドロキシメチルシトシン / シトシン修飾 / 精子 / 発生・分化 / エピジェネティクス
Outline of Final Research Achievements	We have been developing a new technique, Enzyme-assisted Identification of Genome Modification Assay (EnIGMA), which allows us to distinguish between mC and hmC at single-base resolution. In the present study, we applied this method to the analysis of cytosine modification in the mouse sperm genome and aim to identify the conditions that would alter the cytosine modification of the sperm genome. To this end, we have successfully developed a new method, genome-wide EnIGMA-seq, that is capable of identification of cytosine modification status in the genome. In fact, we have shown that this new method is effective for comprehensive cytosine modification of the entire genome.
Academic Significance and Societal Importance of the Research Achievements	この研究で新たに開発したゲノムワイドEnIGMA-seq技術は、１塩基解像度でゲノムのシトシン修飾をメチルシトシン、ヒドロキシメチルシトシン、非修飾のシトシンの３つの状態のいずれであるかを同時に1分子上で開発することのできる手法であり、エピゲノム研究の新しい基盤技術として重要な意義を持つ。