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Functional analysis of novel maternal factors controlling germ cell formation

Research Project

Project/Area Number 17H03686
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionKumamoto University

Principal Investigator

Nakamura Akira  熊本大学, 発生医学研究所, 教授 (90323245)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Keywordsショウジョウバエ / 生殖細胞 / 生殖質 / RNA顆粒 / 母性因子 / RNA局在 / RNP顆粒 / 生殖顆粒
Outline of Final Research Achievements

Germ cells are the only cell type that transmits genetic information to the next generation. In Drosophila, germ cell formation relies on the germ plasm, which is assembled during oogenesis at the posterior pole of the oocyte. We have investigated mechanisms by which the germ plasm is assembled and maintained, as well as those by which germ cell development is operated. Series of our studies have provided several new concepts, including a new physiological roles of yolk uptake into the oocyte and transient transcriptional quiescence in newly formed germ cells. We have also identified new maternal factors that are involved in germ plasm assembly. Studies on these new factors will provide new insights into mechanisms underlying germ cell development in the future.

Academic Significance and Societal Importance of the Research Achievements

本研究では、ショウジョウバエをモデル系として用いて、生殖細胞の形成・分化に関する研究を進めた。一連の成果から、①卵黄タンパク質の受容体を介した取り込みが、単に胚発生過程で必要な養分の蓄積という役割だけでなく、卵形成過程における前後軸の形成維持や生殖質形成に機能するという新しい知見や、②生殖細胞における一過的かつゲノムワイドな転写抑制の生理的意義等が明らかとなった。これら新しい知見は、生命現象を支配する基本的かつ普遍的な制御プロセスの理解に貢献する学術的意義を持つ。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (34 results)

All 2021 2020 2019 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 9 results,  Open Access: 4 results) Presentation (15 results) (of which Int'l Joint Research: 9 results,  Invited: 1 results) Remarks (8 results)

  • [Int'l Joint Research] Institute of Biology (iBV)/CNRS: Nice/CNRS Delegation Languedoc: Montpellier,(フランス)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Copenhagen(デンマーク)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Receptor-mediated yolk uptake is required for oskar mRNA localization and cortical anchorage of germ plasm components in the Drosophila oocyte2021

    • Author(s)
      Tanaka, T., Tani, N. and Nakamura, A.
    • Journal Title

      PLOS Biology

      Volume: 19 Issue: 4 Pages: e3001183-e3001183

    • DOI

      10.1371/journal.pbio.3001183

    • NAID

      120007034423

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tyramine induces dynamic RNP granule remodeling and translation activation in the Drosophila brain2021

    • Author(s)
      Formicola, N., Heim, M., Dufourt, J., Lancelot, A.-S., Nakamura, A., Lagha, M. and Besse, F.
    • Journal Title

      eLIFE

      Volume: 10

    • DOI

      10.7554/elife.65742

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Pgc suppresses the zygotically-acting RNA decay pathway to protect germ plasm RNAs in the Drosophila embryo2019

    • Author(s)
      Hanyu-Nakamura, K., Matsuda, K., Cohen, S. M. and Nakamura, A.
    • Journal Title

      Development

      Volume: 146 Issue: 7

    • DOI

      10.1242/dev.167056

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Rapid and Efficient generation of GFP-knocked-in Drosophila by the CRISPR-Cas9-mediated genome editing.2019

    • Author(s)
      Kina, H., Yoshitani, T., Hanyu-Nakamura, K. and Nakamura, A.
    • Journal Title

      Development Growth & Differentiation

      Volume: 61 Issue: 4 Pages: 265-275

    • DOI

      10.1111/dgd.12607

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Maternal Nanos inhibits Importin-α2/Pendulin-dependent nuclear import to prevent somatic gene expression in the Drosophila germline2019

    • Author(s)
      Asaoka Miho、Hanyu-Nakamura Kazuko、Nakamura Akira、Kobayashi Satoru
    • Journal Title

      PLOS Genetics

      Volume: 15 Issue: 5 Pages: e1008090-e1008090

    • DOI

      10.1371/journal.pgen.1008090

    • NAID

      120007133528

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Overlapping function of klf family targets multiple transcription factors to maintain naive pluripotency of ES cells2018

    • Author(s)
      Yamane Mariko、Ohtsuka Satoshi、Matsuura Kumi、Nakamura Akira、Niwa Hitoshi
    • Journal Title

      Development

      Volume: 印刷中 Issue: 10

    • DOI

      10.1242/dev.162404

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Map7/7D1 and Dvl form a feedback loop that facilitates microtubule remodeling and Wnt5a signaling.2018

    • Author(s)
      Kikuchi K, Nakamura A, Arata M, Shi D, Nakagawa M, Tanaka T, Uemura T, Fujimori T, Kikuchi A, Uezu A, Sakamoto Y, Nakanishi H.
    • Journal Title

      EMBO Rep.

      Volume: 19(7) Issue: 7

    • DOI

      10.15252/embr.201745471

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Germ-cell formation in solitary ascidians: Coexistance of preformation and epigenesist.2018

    • Author(s)
      Shirae-Kurabayashi, M. and Nakamura, A.
    • Journal Title

      In: Reproductive and Developmental Strategies (Kobayashi, K. et al eds) Springer-Nature

      Volume: - Pages: 3-18

    • DOI

      10.1007/978-4-431-56609-0_1

    • ISBN
      9784431566076, 9784431566090
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Map7/7D1 and Dvl form a feedback loop that facilitates microtubule remodeling and Wnt5a signaling.2018

    • Author(s)
      Kikuchi, K., Nakamura, A., Arata, M., Shi, D., Nakagawa, M., Tanaka, T., Uemura, T., Fujimori, T., Kikuchi, A., Uezu, A., Sakamoto, Y. and Nakanishi, H.
    • Journal Title

      EMBO Report

      Volume: 印刷中

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] Receptor-mediated yolk uptake directs Drosophila oocyte polarization and germ plasm assembly2020

    • Author(s)
      T. Tanaka, S. Otsu, N. Tani and A. Nakamura,
    • Organizer
      JSDB Online Trial Meeting 2020
    • Related Report
      2020 Annual Research Report
  • [Presentation] Spatiotemporal regulation of Map7D1 expression and dynamics is involved in cell polarity formation during mammalian tissue morphogenesis2020

    • Author(s)
      K. Kikuchi, M. Nakagawa, T. Fujimori, J. Hatakeyama, H. Sato, K. Shimamura, K. Ohta, A. Nakamura, M. Suzuki and H. Nakanishi
    • Organizer
      JSDB Online Trial Meeting 2020
    • Related Report
      2020 Annual Research Report
  • [Presentation] The Drosophila PGC-1 homolog, Spargel, is required for proper germ granule assembly during oogenesis2020

    • Author(s)
      K. Hanyu-Nakamura, K. Aimi and A. Nakamura
    • Organizer
      JSDB Online Trial Meeting 2020
    • Related Report
      2020 Annual Research Report
  • [Presentation] Drosophila Tpp ensures the germ plasm assembly by facilitating the posterior localization of Aubergine in the oocyte2019

    • Author(s)
      Hirono Kina, Hina Nakashima, Tsubasa Tanaka Kazuko Hanyu-Nakamura and Akira Nakamura
    • Organizer
      The 52nd Annual meeting of Japanese Society of Developmental Biologists
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ショウジョウバエ生殖質形成を促進する新規小タンパク質2019

    • Author(s)
      中村 輝
    • Organizer
      2019遺伝研・熊大発生研共催シンポジム「有性生殖における染色体・クロマチン・核動態」
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ショウジョウバエTppタンパク質によるAubergineの生殖質への集積促進メカニズムの解析2019

    • Author(s)
      喜納 寛野1、中島向南、羽生 賀津子、田中 翼、中村 輝
    • Organizer
      第21回日本RNA学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Drosophila tiny pole plasm encodes an evolutionary conserved protein that facilitates posterior localization of Aubergine during germ plasm assembly in the oocyte2019

    • Author(s)
      ◯A. Nakamura, H. Kina, T. Yoshitani, T. Tanaka, K. and Hanyu-Nakamura.
    • Organizer
      The 20th Takeda Science Foundation Symposium on Bioscience: RNA Neobiology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of a new maternal factor involved in germ cell formation in the Drosophila embryos2018

    • Author(s)
      T. Yoshitani, H. Kina, T. Tanaka, K. Hanyu-Nakamura and ◯A. Nakamura.
    • Organizer
      Tokyo2018 Cell and Developmental Biology Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ショウジョウバエtiny pole plasmは小タンパク質をコードし生殖質形成におけるAubergineの卵母細胞後極への輸送を促進する2018

    • Author(s)
      喜納 寛野、吉谷 崇、田中 翼、羽生-中村 賀津子、○中村 輝
    • Organizer
      第20回日本RNA学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Drosophila tiny pole plasm encodes a small protein that facilitates posterior localization of Aubergine during germ plasm assembly in the oocyte2018

    • Author(s)
      H. Kina, T. Yoshitani, T. Tanaka, K. Hanyu-Nakamura and ◯A. Nakamura.
    • Organizer
      The 13th Japanese Drosophila Research Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of a new maternal factor involved in germ cell formation in the Drosophila embryos.2018

    • Author(s)
      T. Yoshitani, H. Kina, T. Tanaka, K. Hanyu-Nakamura, and A. Nakamura
    • Organizer
      The 51th Annual Meeting of the Japanese Society of Developmental Biologists
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Isolation and characterization of novel maternal-effect genes regulating germ cell development in the Drosophila embryo.2017

    • Author(s)
      K. Aimi, K. Hanyu-Nakamura, and A. Nakamura
    • Organizer
      The 50th Annual Meeting of the Japanese Society of Developmental Biologists
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The endocytic regulation of the yolk protein receptor Yolkless is required for the polarity establishment and germ plasm assembly in the Drosophila oocyte.2017

    • Author(s)
      T. Tanaka, S. Otsu, N. Tani, and A. Nakamura
    • Organizer
      The 50th Annual Meeting of the Japanese Society of Developmental Biologists
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ligand-induced endocytosis of the yplk-protein receptor Yolkless is required for the oocyte polarization and germ plasm assembly in Drosophila.2017

    • Author(s)
      T. Tanaka, S. Otsu, N. Tani, and A. Nakamura
    • Organizer
      The International Research Symposium on Regulation of Germ Cell Development In Vivo and In Vitro
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Roles of yolk uptake in the Drosophila oocyte polarization and germ plasm assembly.2017

    • Author(s)
      T. Tanaka, S. Otsu, N. Tani, and A. Nakamura
    • Organizer
      KEY FORUM: The 3rd International Symposium on Stem Cell Traits and Developmental Systems
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 熊本大学発生医学研究所 生殖発生分野

    • URL

      http://www.imeg.kumamoto-u.ac.jp/bunya_top/germline_development/

    • Related Report
      2020 Annual Research Report
  • [Remarks] 卵黄の常識が変わる:卵母細胞の機能における卵黄タンパク質の取り込みの重要性を発見

    • URL

      https://www.kumamoto-u.ac.jp/whatsnew/seimei/20210424

    • Related Report
      2020 Annual Research Report
  • [Remarks] 発生医学研究所生殖発生分野

    • URL

      http://www.imeg.kumamoto-u.ac.jp/bunya_top/germline_development/

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
  • [Remarks] ショウジョウバエPgcタンパク質は、生殖質を構成するmRNAを分解から保護する

    • URL

      http://www.imeg.kumamoto-u.ac.jp/np100/

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
  • [Remarks] CRISPR-Cas9遺伝子改変技術を用いた効率的なショウジョウバエGFPノックイン系統の作出

    • URL

      http://www.imeg.kumamoto-u.ac.jp/np101/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 生殖細胞が作られる過程では、体を作る細胞の生成が抑制される

    • URL

      http://www.imeg.kumamoto-u.ac.jp/nakamura2019may/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 組織の形成に関わる、細胞の「かたち」や「ならび」を調節する新しい仕組みの解明

    • URL

      http://www.imeg.kumamoto-u.ac.jp/kikuchi2018jun/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 発生医学研究所分野紹介ー生殖発生分野

    • URL

      http://www.imeg.kumamoto-u.ac.jp/bunya_top/germline_development/

    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2022-01-27  

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