| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2019: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2018: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2017: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Outline of Final Research Achievements |
Myst family genes encode lysine acetyltransferases that mainly mediate histone acetylation to control transcription, DNA replication and DNA damage response. They form tetrameric complexes with PHD-finger proteins (Brpfs or Jades) and small non-catalytic subunits Ing4/5 and Meaf6. Although all the components of the complex are well-conserved from yeast to mammals, the function of Meaf6 and its homolog has not been elucidated in any species. Here we revealed the role of Meaf6 in the Myst complex utilizing inducible Meaf6 KO ES cells. Elimination of Meaf6 results in cease of proliferation although histone acetylation is largely unaffected. In the absence of Meaf6, one of the Myst family members Myst2 increased its ability to interact with PHD-finger proteins. This is the first indication of the function of Meaf6, which is not essential for HAT activity, but modulates the stoichiometry of the Myst complex.
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