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Gene regulatory network in the epiblast regionalization and somatic lineage derivation

Research Project

Project/Area Number 17H03688
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionKyoto Sangyo University

Principal Investigator

KONDOH Hisato  京都産業大学, 総合学術研究所, 科研費研究員 (70127083)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Keywordsエピブラスト / 体細胞発生 / 神経系 / 内胚葉 / 転写因子 / 幹細胞 / ニワトリ胚 / マウス胚 / 領域特性 / 神経幹細胞 / 内胚葉前駆体 / 原腸陥入 / 細胞分化
Outline of Final Research Achievements

Any somatic tissue has its developmental origin in the pluripotent epiblast. We investigated the early stages of somatic tissue development focusing on the neural and endodermal primordia.
Anterior epiblast cells already have regionalities of future brain portions when the node develops. As the epiblast cells gather upon the node-derived anterior mesendoderm, they form the neural primordium already with the traits of brain portions. The higher Wnt signal level in the posterior embryo sites determines the posterior regionality in the hindbrain and spinal cord. Further neural development from the primordium is promoted by cooperative actions of the SOX2 and ZIC2 transcription factors.
Interaction of the SOX17-expressing fraction of the epiblast cells with the laminin-rich basement membrane elicits the genesis of the endodermal primordium. The action of the esophageal SOX2 against the tracheal development is essential for the esophagus/trachea separation in the anterior endoderm.

Academic Significance and Societal Importance of the Research Achievements

ヒトの胚発生では、受精後1ヶ月の間に、エピブラストという体細胞全体の前駆体から様々な体細胞組織の原基が発生し成立する。胚発生の最も重要な時期である。この時期の発生が順調に進行しないと、さまざまな先天異常の原因にもなる。この時期の胚発生の主要なプロセス、ニワトリ胚、マウス胚、マウス胚から樹立したエピブラスト幹細胞株を用いて研究した。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (48 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (1 results) Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 9 results,  Open Access: 6 results) Presentation (34 results) (of which Int'l Joint Research: 20 results,  Invited: 7 results) Remarks (4 results)

  • [Int'l Joint Research] Emory University of Medicine(米国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] The formation of multiple pituitary pouches from the oral ectoderm causes ectopic lens development in hedgehog signaling-defective avian embryos.2020

    • Author(s)
      Taira Y, Ikuta Y, Inamori S, Nunome M, Nakano M, Suzuki T, Matsuda Y, Tsudzuki M, Teramoto M, Iida H, Kondoh H.
    • Journal Title

      Developmental Dynamics

      Volume: 249 Issue: 12 Pages: 1425-1439

    • DOI

      10.1002/dvdy.222

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Modeling early stages of endoderm development in epiblast stem cell aggregates with supply of extracellular matrices.2020

    • Author(s)
      Inamori S, Fujii M, Satake S, Iida H, Teramoto M, Sumi T, Meno C, Ishii Y, Kondoh H.
    • Journal Title

      Dev Growth Differ.

      Volume: - Issue: 4 Pages: 243-259

    • DOI

      10.1111/dgd.12663

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sox2 gene regulation via the D1 enhancer in embryonic neural tube and neural crest by the combined action of SOX2 and ZIC22020

    • Author(s)
      Iida Hideaki、Furukawa Yoko、Teramoto Machiko、Suzuki Hitomi、Takemoto Tatsuya、Uchikawa Masanori、Kondoh Hisato
    • Journal Title

      Genes to Cells

      Volume: In press Issue: 4 Pages: 242-256

    • DOI

      10.1111/gtc.12753

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The absence of SOX2 in the anterior foregut alters the esophagus into trachea and bronchi in both epithelial and mesenchymal components2020

    • Author(s)
      Teramoto Machiko、Sugawara Ryo、Minegishi Katsura、Uchikawa Masanori、Takemoto Tatsuya、Kuroiwa Atsushi、Ishii Yasuo、Kondoh Hisato
    • Journal Title

      Biology Open

      Volume: 9 Pages: 048728-048728

    • DOI

      10.1242/bio.048728

    • NAID

      120006859990

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cooperation of Sall4 and Sox8 transcription factors in the regulation of the chicken Sox3 gene during otic placode development2018

    • Author(s)
      Okamoto Yu、Nishimura Naoko、Matsuda Kazunari、Ranawakage Deshani C.、Kamachi Yusuke、Kondoh Hisato、Uchikawa Masanori
    • Journal Title

      Development, Growth & Differentiation

      Volume: 60 Issue: 3 Pages: 133-145

    • DOI

      10.1111/dgd.12427

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Roles of ZIC2 in Regulation of Pluripotent Stem Cells2018

    • Author(s)
      Kondoh Hisato
    • Journal Title

      Adv Exp Med Biol.

      Volume: 1046 Pages: 339-351

    • DOI

      10.1007/978-981-10-7311-3_17

    • ISBN
      9789811073106, 9789811073113
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nasal and otic placode specific regulation of Sox2 involves both activation by Sox-Sall4 synergism and multiple repression mechanisms2018

    • Author(s)
      Sugahara Satoko、Fujimoto Tooru、Kondoh Hisato、Uchikawa Masanori
    • Journal Title

      Developmental biology

      Volume: 433 Issue: 1 Pages: 61-74

    • DOI

      10.1016/j.ydbio.2017.11.005

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhancer Analyses Using Chicken Embryo Electroporation2017

    • Author(s)
      Uchikawa Masanori、Nishimura Naoko、Iwafuchi-Doi Makiko、Kondoh Hisato
    • Journal Title

      Methods in molecular biology (Clifton, N.J.)

      Volume: 1650 Pages: 191-202

    • DOI

      10.1007/978-1-4939-7216-6_12

    • ISBN
      9781493972159, 9781493972166
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ChIP-seq analysis of genomic binding regions of five major transcription factors highlights a central role for ZIC2 in the mouse epiblast stem cell gene regulatory network2017

    • Author(s)
      Matsuda Kazunari、Mikami Tomoyuki、Oki Shinya、Iida Hideaki、Andrabi Munazah、Boss Jeremy M.、Yamaguchi Katsushi、Shigenobu Shuji、Kondoh Hisato
    • Journal Title

      Development

      Volume: 144 Pages: 1948-1958

    • DOI

      10.1242/dev.143479

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] The formation of multiple pituitary pouches from the oral ectoderm in hedgehog signaling-defective avian embryos causing ectopic lens development2020

    • Author(s)
      Yuki Taira, Yuya Ikuta, Machiko Teramoto, Mitsuo Nunome, Mikiharu Nakano, Takayuki Suzuki, Yoichi Matsuda, Masaoki Tsudzuki, Hideaki Iida, Hisato Kondoh
    • Organizer
      53rd Annual Meeting of JSDB
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Live imaging of avian epiblast development using randomly or selectively marked cell populations2020

    • Author(s)
      Hisato Kondoh, Hideaki Iida, Koya Yoshihi, Kagayaki Kato
    • Organizer
      53rd Annual Meeting of JSDB
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Possible Sox2 autoregulation involving POU partner factors in the establishment of embryonic neural primordia2019

    • Author(s)
      Hideaki Iida , Masanori Uchikawa, Hisato Kondoh
    • Organizer
      52nd Annunal Meeting of Japanese Society of Developmental Biologists
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Endodermal SOX2 expression determines the esophagus character of the anterior foregut in both epithelial and mesenchymal components2019

    • Author(s)
      Machiko Teramoto, Ryo Sugawara, Atsushi Kuroiwa, Yasuo Ishii, Hisato Kondoh
    • Organizer
      52nd Annunal Meeting of Japanese Society of Developmental Biologists
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A new fate map and anterior mesendoderm-dependent regulation of brain precursor development determined via live imaging of avian embryos2019

    • Author(s)
      Hisato Kondoh, Koya Yoshihi, Hideaki Iida, Machiko Teramoto, Kagayaki Kato
    • Organizer
      52nd Annunal Meeting of Japanese Society of Developmental Biologists
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Changing SOX partners during the developmental progression of stem cells2019

    • Author(s)
      Hisato Kondoh
    • Organizer
      Vth International SOX Research Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 光褪色後蛍光回復法(FRAP)による神経幹細胞内のSOX2のパートナー転写因子との相互作用の解析2019

    • Author(s)
      渡邊 優作, 中村 香絵, 三上 智之, 近藤 寿人
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 単一エンハンサー内でのSOX2-ZIC2、SOX2-PAX3の2つの転写因子相互作用によって、Sox2遺伝子が神経系と神経堤で活性化される2019

    • Author(s)
      飯田 英明, 内川 昌則, 近藤 寿人
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Modeling node-proximal gastrulation in epiblast stem cells2019

    • Author(s)
      Mai Fujii, Sachiko Inamori, Machiko Teramoto, Hideaki Iida, Hisato Kondoh
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 食道上皮でのSOX2発現が失われると、上皮だけでなく間充織までも気管と気管支に変わる2019

    • Author(s)
      寺元 万智子, 菅原 諒, 黒岩 厚, 石井 泰雄, 近藤 寿人
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] A new live imaging technique of avian embryos determining a revised fate map and mesendoderm-dependent gathering of brain precursors2019

    • Author(s)
      Hisato Kondoh, Koya Yoshihi, Machiko Teramoto, Hideaki Iida, Kagayaki Kato
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Mechanisms underlying lens transdifferentiation from neural retina and pituitary2018

    • Author(s)
      Hisato Kondoh
    • Organizer
      Gordon Research Conference Visual System Development: Genetics, Genomics, and Evolution of the Eye
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Live imaging of node graft and host cells to establish its role in head development2018

    • Author(s)
      Koya Yoshihi, Kagayaki Kato, Hisato Kondoh
    • Organizer
      Joint Annual Meeting of 51st JSDB and 70th JSCB
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regulation of a pan-neural Sox2 enhancer D12018

    • Author(s)
      Hideaki Iida, Masanori Uchikawa, Hisato Kondoh
    • Organizer
      Joint Annual Meeting of 51st JSDB and 70th JSCB
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] SOX2-dependent determination of tissue identities in the foregut2018

    • Author(s)
      Machiko Teramoto, Ryo Sugawara, Atsushi Kuroiwa, Yasuo Ishii, Hisato Kondoh
    • Organizer
      Joint Annual Meeting of 51st JSDB and 70th JSCB
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The pairing interaction dynamics of the transcription factor SOX2 with partner factors2018

    • Author(s)
      Hisato Kondoh
    • Organizer
      International Symposium on “Proteins; from the Cradle to the Grave”
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Mechanisms underlying lens transdifferentiation in neural retina cells and pituitary precursors2018

    • Author(s)
      Hisato Kondoh
    • Organizer
      XXIII Biennial Meeting of the International Society for Eye Research
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] A drastic change in the major regulatory TFs from SOX2- POU5F1 to ZIC2-OTX2 in the pluripotent stem cells representing pre-implantation and post-implantation stages2018

    • Author(s)
      Hisato Kondoh
    • Organizer
      EMBO Workshop: Molecular Mechanisms of Developmental and Regenerative Biology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] D1エンハンサーによる、胚の中枢神経系と頭部神経堤におけるSox2発現の制御2018

    • Author(s)
      飯田 英明、内川 昌則、近藤 寿人
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 内胚葉で発現されるSOX2が前腸の上皮と間充織の双方を食道に発生させる2018

    • Author(s)
      寺元 万智子、菅原 諒、黒岩 厚、石井 泰雄、近藤 寿人
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] エピブラスト幹細胞を用いて、着床後に体細胞系列が生み出される機構を研究する2018

    • Author(s)
      近藤 寿人、中村 香絵、渡邊 優作、藤井 麻衣、稲森 祥子、飯田 英明
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] SOX2-dependent determination of tissue identity in the foregut.2017

    • Author(s)
      Teramoto M, Sugawara R, Ishii Y, Kuroiwa A, Kondoh H.
    • Organizer
      ConBio2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] A drastic change in the major regulatory TFs from SOX2-POU5F1 to ZIC2-OTX2 in the pluripotent stem cells representing pre-implantation and post-implantation stages.2017

    • Author(s)
      Kondoh H, Matsuda K, Oki S, Shigenobu S.
    • Organizer
      ConBio2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] エピブラスト幹細胞株から樹立された領域特性をもつ神経幹細胞株の解析2017

    • Author(s)
      中村香絵、Boitet C、吉氷康矢、飯田英明、近藤寿人
    • Organizer
      ConBio2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] Foxa2とともにnEGFPを発現するエピブラスト幹細胞を用いた、初期発生過程の研究2017

    • Author(s)
      稲森祥子、石井泰雄、近藤寿人
    • Organizer
      ConBio2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヘンゼン結節は脊索前板の発生を介して、Otx2を発現するエピブラストに頭部を形成させる2017

    • Author(s)
      吉氷康矢、近藤寿人
    • Organizer
      ConBio2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] Sox2の神経系での発現を制御するD1エンハンサーの解析2017

    • Author(s)
      飯田英明、内川昌則、近藤寿人
    • Organizer
      ConBio2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] SOX2-dependent determination of tissue identity in the foregut.2017

    • Author(s)
      Teramoto M, Sugawara R, Ishii Y, Kuroiwa A, Kondoh H.
    • Organizer
      ISDB 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Roles for ZIC2 in the regulation of epiblast stem cells and embryonic stem cells.2017

    • Author(s)
      Kondoh H, Matsuda K, Oki S, Shigenobu S.
    • Organizer
      ISDB 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Embryonic region-dependent development and interactions of grafted Hensen’s node with host tissues: reconsideration of organizer model.2017

    • Author(s)
      Kondoh H, Yoshihi K.
    • Organizer
      50th Annual Meeting of the Japanese society of Developmental Biologists.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Intrinsic lens potential of neural retina inhibited by Notch signaling as the cause of “lens transdifferentiation”.2017

    • Author(s)
      Iida H, Ishii Y, Kondoh H.
    • Organizer
      50th Annual Meeting of the Japanese society of Developmental Biologists.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Establishment of neural progenitor cells lines with defined regional specificities from EpiSCs.2017

    • Author(s)
      Nakamura K, Boitet C, Satake S, Yoshihi K, Iida H, Ishii Y, Kondoh H.
    • Organizer
      50th Annual Meeting of the Japanese society of Developmental Biologists.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Establishment and use of a new epiblast stem cell line marking Foxa2 expression with GFP.2017

    • Author(s)
      Inamori S, Ishii Y, Kondoh H.
    • Organizer
      50th Annual Meeting of the Japanese society of Developmental Biologists.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] SOX2-dependent determination of tissue identity in the foregut.2017

    • Author(s)
      Teramoto M, Sugawara R, Ishii Y, Kuroiwa A, Kondoh H.
    • Organizer
      50th Annual Meeting of the Japanese society of Developmental Biologists.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 再生医療の地図を描くエピブラスト幹細胞

    • URL

      https://www.kyoto-su.ac.jp/faculty/professors/nls/ahcetq00000023w2-att/st_kondo.pdf

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
  • [Remarks] Laboratory of Developmental Biology 発生生物学研究室

    • URL

      https://www.cc.kyoto-su.ac.jp/~i1659235/HKlab/index.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] SOX2に導かれた発生の遺伝子制御への道

    • URL

      https://www.kyoto-su.ac.jp/graduate/g_ls/kenkyu/kondo.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 教員紹介 教授 近藤寿人

    • URL

      https://www.kyoto-su.ac.jp/graduate/g_ls/kyoin/kondo.html

    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2022-05-23  

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