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A new molecular model for plant cell wall based on a new enzyme, cellulose endotransglucosylase

Research Project

Project/Area Number 17H03691
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionKanagawa University (2019)
Tohoku University (2017-2018)

Principal Investigator

Nishitani Kazuhiko  神奈川大学, 理学部, 教授 (60164555)

Co-Investigator(Kenkyū-buntansha) 大林 武  東北大学, 情報科学研究科, 准教授 (50397048)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2017: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords細胞壁 / XTH / セルロース / プロトプラスト / 細胞壁再生 / キシログルカン / 植物 / 酵素反応 / 植物細胞壁 / 酵素 / 細胞壁モデル / セルロース繋ぎ換え酵素 / CET
Outline of Final Research Achievements

The molecular mechanism underlying the physical integration of cellulose microfibrils into the network architecture still remains controversial. To gain insight into this dilemma, we first investigated distribution of cellulose endotransglucosylase in land plants by phylogeny. Next, we investigated the cell wall regeneration process using mesophyll protoplasts derived from xyloglucan less mutant leaves. Based on the results obtained we propose that xyloglucan is not essential for the initial assembly of the cellulose network, and that cellulose network formation as mediated by cellulose endotransglucosylase might play a role in the land plants.

Academic Significance and Societal Importance of the Research Achievements

陸上植物の細胞壁は、植物の発生・分化や成長のみならず、植物の生体防御や寄生、共生における種間の認識、環境との相互作用や摂取など、陸上環境での植物の主要な機能のすべてに関わる細胞装置である。また、陸上植物の細胞壁は、陸上のバイオマスの8割を占める循環型炭素資源であり、その根幹をなすのがセルロース微繊維である。その重要性にも拘らず、細胞壁内でのセルロース微繊維のネットワーク構築メカニズムは今尚未解明のままである。今回の本研究の成果は、この未解明の問題を解明する重要な知見を与えるもので、基礎科学と技術革新の両面において、社会的意義が大きいと考えられる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (50 results)

All 2020 2019 2018 2017 Other

All Journal Article (12 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 10 results,  Open Access: 9 results) Presentation (33 results) (of which Int'l Joint Research: 8 results,  Invited: 1 results) Book (2 results) Remarks (3 results)

  • [Journal Article] Xyloglucan is not essential for the formation and integrity of the cellulose network in the primary cell wall regenerated from Arabidopsis protoplasts.2020

    • Author(s)
      Kuki, H., Yokoyama, R., Kuroha,T., Nishitani, K.
    • Journal Title

      Plants

      Volume: 9 Issue: 5 Pages: 629-629

    • DOI

      10.3390/plants9050629

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Root-knot nematodes modulate cell walls during root-knot formation in Arabidopsis roots.2020

    • Author(s)
      Ishida, T., Suzuki, R., Nakagami, S., Kuroha, T., Sakamoto, S., Nakata, M.T., Yokoyama, R., Kimura, S., Mitsuda, N., Nishitani, K., Sawa, S.
    • Journal Title

      J. Plant Res.

      Volume: 133 Issue: 3 Pages: 419-428

    • DOI

      10.1007/s10265-020-01186-z

    • NAID

      40022231088

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interspecific signaling between the parasitic plant and the host plants regulate xylem vessel cell differentiation in haustoria of Cuscuta campestris.2020

    • Author(s)
      Kaga Y., Yokoyama R., Sano, R., Ohtani, M., Demura, T., Kuroha, T., Shinohara, N., Nishitani, K..
    • Journal Title

      Frontiers in Plant Science

      Volume: 11 Pages: 193-193

    • DOI

      10.3389/fpls.2020.00193

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A conserved regulatory mechanism mediates the convergent evolution of plant shoot lateral organs.2019

    • Author(s)
      Naramoto S, Jones VAS, Nicola, N, Sato M,Toyooka K, Shimamura M, Ishida S, Nishitani K,Ishizaki K, Nishihama R, Kohchi T, Dolan L, Kyozuka J.
    • Journal Title

      PLoS Biology

      Volume: 17 Issue: 12 Pages: 12-12

    • DOI

      10.1371/journal.pbio.3000560

    • NAID

      120006813343

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Pectin RG-I rhamnosyltransferases represent a novel plant-specific glycosyltransferase family2018

    • Author(s)
      Takenaka Yuto、Kato Kohei、Ogawa-Ohnishi Mari、Tsuruhama Kana、Kajiura Hiroyuki、Yagyu Kenta、Takeda Atsushi、Takeda Yoichi、Kunieda Tadashi、Hara-Nishimura Ikuko、Kuroha Takeshi、Nishitani Kazuhiko、Matsubayashi Yoshikatsu、Ishimizu Takeshi
    • Journal Title

      Nature Plants

      Volume: 4 Issue: 9 Pages: 669-676

    • DOI

      10.1038/s41477-018-0217-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ethylene-gibberellin signaling underlies adaptation of rice to periodic flooding2018

    • Author(s)
      T Kuroha, K Nagai, R Gamuyao, D R Wang, T Furuta, M Nakamori, T Kitaoka, K Adachi, A Minami, Y Mori, K Mashiguchi, Y Seto, S Yamaguchi, M Kojima, H Sakakibara, J Wu, K Ebana, N Mitsuda, M Ohme-Takagi, S Yanagisawa, M Yamasaki, R Yokoyama, K Nishitani, T Mochizuki, G Tamiya, S R McCouch, and M Ashikari
    • Journal Title

      Science

      Volume: 361 Issue: 6398 Pages: 181-186

    • DOI

      10.1126/science.aat1577

    • NAID

      120006529624

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 新規酵素、エンド型セルロース転移酵素(CET)を用いた高機能性セルロース素材作出の可能性について2018

    • Author(s)
      西谷和彦
    • Journal Title

      紙パルプ技術タイムス

      Volume: 61 Pages: 63-68

    • NAID

      40021417711

    • Related Report
      2017 Annual Research Report
  • [Journal Article] セルロース分子を繋ぎ換える酵素の発見2018

    • Author(s)
      西谷和彦
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 76 Pages: 42-43

    • Related Report
      2017 Annual Research Report
  • [Journal Article] The plant cell-wall enzyme AtXTH3 catalyses covalent cross-linking between cellulose and cello-oligosaccharide2017

    • Author(s)
      Shinohara, N.; Sunagawa, N.; Tamura, S.; Yokoyama, R.; Ueda, M.; Igarashi, K.; Nishitani, K.
    • Journal Title

      Sci. Rep.

      Volume: 7 Issue: 1 Pages: 46099-46099

    • DOI

      10.1038/srep46099

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Arabinogalactan proteins accumulate in the cell walls of searching hyphae of the stem parasitic plants, Cuscuta campestris and Cuscuta japonica.2017

    • Author(s)
      Hozumi A, Bera S, Fujiwara D, Obayashi T, Yokoyama R, Nishitani K, Aoki K
    • Journal Title

      Plant and Cell Physiology

      Volume: 58 Issue: 11 Pages: 1868-1877

    • DOI

      10.1093/pcp/pcx121

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Insights into Land Plant Evolution Garnered from the Marchantia polymorpha Genome.2017

    • Author(s)
      Bowman J, Kouchi T,..(106名)..Yokoyama R, ..(4名)
    • Journal Title

      Cell

      Volume: 171 Issue: 2 Pages: 287-304

    • DOI

      10.1016/j.cell.2017.09.030

    • NAID

      120006353839

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Quantitative confocal imaging method for analyzing cellulose dynamics during cell-wall regeneration in Arabidopsis mesophyll protoplasts.2017

    • Author(s)
      Kuki H, Higaki T, Yokoyama R, Kuroha T, Shinohara N, Hasezawa S, Nishitani K
    • Journal Title

      Plant Direct

      Volume: 1 Issue: 6 Pages: 1-10

    • DOI

      10.1002/pld3.21

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Involvement of Xyloglucan Endotransglucosylase/Hydrolase in Plant Freezing Tolerance2019

    • Author(s)
      Takahashi,D., Sampathkumar,A., Yokoyama, R., Kuroha,T., Nishitani, K., Zuther,E., Hincha,D.K.
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ゼニゴケにおける Endoglucanase16 の機能解析2019

    • Author(s)
      石田光南,黒羽剛,石崎公庸,檜垣匠,楢本悟史,横山隆亮,小竹敬久,西谷和彦
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 炭素イオンビームによる寄生植物ネナシカズラ属の変異体ライブラリ作製2018

    • Author(s)
      横山隆亮,加賀悠樹,加藤萌木,古井瑛恵,黒羽剛,西谷和彦,佐藤勝也、大野豊
    • Organizer
      高崎サイエンスフェスタ2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] 基部陸上植物における細胞壁酵素(GH16)の進化2018

    • Author(s)
      石田光南,黒羽剛,石崎公庸,檜垣匠,楢本悟史,横山隆亮,西谷和彦
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 細胞壁再生イメージング解析法によるキシログルカンの機能解析2018

    • Author(s)
      九鬼寛明,横山隆亮,黒羽剛,西谷和彦
    • Organizer
      東北植物学会第8回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] イネ強稈化に関わる量的形質遺伝子座qGF1の機能解析2018

    • Author(s)
      保浦徳昇,黒羽 剛,西谷和彦,北野英己
    • Organizer
      イネ遺伝学・分子生物学ワークショップ
    • Related Report
      2018 Annual Research Report
  • [Presentation] 寄生植物ネナシカズラのゲノムから読解く生命戦略2018

    • Author(s)
      西谷和彦
    • Organizer
      イネ遺伝学・分子生物学ワークショップ
    • Related Report
      2018 Annual Research Report
  • [Presentation] 長期的な洪水に適応した浮きイネの起源とその栽培化2018

    • Author(s)
      黒羽 剛, Diane Wang, Susan R. McCouch, 横山隆亮, 西谷和彦, 芦苅基行
    • Organizer
      日本育種学会第134回講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 寄生植物 アメリカネナシカズラの花成制御におけるFTの機能2018

    • Author(s)
      加藤萌木,横山隆亮,黒羽剛,大林武,阿部光知,西谷和彦
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 植物種間情報伝達を介したアメリカネナシカズラ吸器における維管束新生機構の探索2018

    • Author(s)
      加賀悠樹,大林武,横山隆亮,黒羽剛,大谷美沙都,出村拓,西谷和彦
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 浮イネにおけるジベレリン合成を介した洪水への適応戦略2018

    • Author(s)
      黒羽剛,増口潔,瀬戸義哉,山口信次郎,横山隆亮,西谷和彦
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 植物細胞壁の再編酵素群の機能とその応用の可能性2018

    • Author(s)
      西谷和彦
    • Organizer
      植物バイオの先端技術(1)セミナー植物バイオ研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Molecular insights into changes of the extracellular matrix during cold and sub-zero acclimation2018

    • Author(s)
      Takahashi,D., Sampathkumar,A., Erban,A., Gorka,M., Kopka,J., Graf,A., Yokoyama, R., Kuroha,T., Nishitani, K., Zuther,E., Hincha,D.K.
    • Organizer
      11th International Plant Cold Hardiness Seminar
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 新規糖転移酵素ファミリーGT106を構成するぺクチン生合成酵素の機能解析2018

    • Author(s)
      竹中悠人,加藤耕平,小川(大西)真里,鶴浜加奈,梶浦裕之,柳生健太,竹田篤史,武田陽一,國枝 正,西村いくこ,黒羽 剛,西谷和彦,松林嘉克,石水 毅
    • Organizer
      第19回関西グライコサイエンスフォーラム
    • Related Report
      2018 Annual Research Report
  • [Presentation] Insight into strategies for plant-plant parasitism in Cuscuta campestris based on genome sequencing.2018

    • Author(s)
      Narukawa, H., Yokoyama, R., Obayashi, T., Kaga, Y., Kato, M., Kuroha, T., Nishitani, K.
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 洪水環境に適応した浮イネの進化におけるジベレリン合成遺伝子の関与2018

    • Author(s)
      黒羽剛,ダイアンワン,スーザンマッコーチ,横山隆亮,西谷和彦,芦苅基行
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] アメリカネナシカズラのゲノム解読による寄生植物の適応進化メカニズムの解明2018

    • Author(s)
      横山隆亮,大林武,鳴川秀樹,加賀悠樹,加藤萌木,黒羽剛,西谷和彦
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] アメリカネナシカズラが持つ付着器の形成に関与する遺伝子の探索2018

    • Author(s)
      藤原大輝,横山隆亮,西谷和彦,青木考
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Apoplastic and symplastic interactions between parasitic plants and host plants2017

    • Author(s)
      Aoki, K., Hozumi, A., Ekawa, M., Shimizu, K., Yokoyama, R., Nishitani, K.
    • Organizer
      14th World Congress on Parasitic Plants Asilomar USA
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 植物細胞壁ペクチン成分ラムノガラクツロナンIの生合成に関与するラムノース転移酵素遺伝子の同定2017

    • Author(s)
      加藤耕平,西谷和彦,石水毅 他
    • Organizer
      第36回日本糖質学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ネナシカズラのゲノムから読み解く茎寄生の生命戦略2017

    • Author(s)
      西谷和彦,横山隆亮,大林武 他
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 比較ゲノム解析から見えてきた茎寄生植物ネナシカズラの形態形成メカニズム2017

    • Author(s)
      横山隆亮,大林武,鳴川秀樹,加賀悠樹,加藤萌木,黒羽剛,西谷和彦
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 浮きイネの深水応答機構における進化的考察2017

    • Author(s)
      黒羽剛,Wang Diane,Gamuyao Rico ,永井啓祐, 横山隆亮,西谷和彦,McCouch Susan R. ,芦苅基行
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 茎寄生植物ネナシカズラにおける宿主由来エチレンを介した吸器発生メカニズム2017

    • Author(s)
      鳴川秀樹,加賀悠樹,横山隆亮,黒羽剛,西谷和彦
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 細胞壁イメージングと新規の酵素機能の解析に基づく新しい細胞壁高次構造モデルの提案2017

    • Author(s)
      九鬼寛明,桧垣匠,篠原直貴,横山隆亮,黒羽剛,三上慎吾,馳澤盛一郎,西谷和彦
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 異なる光条件におけるペクチン分解酵素過剰発現イネを用いたオリゴガラクツロン酸誘導性病害応答2017

    • Author(s)
      竹内春樹,木村郷子,小原崇司,佐藤淳也,中村敦子,出崎能丈,渋谷直人,賀来華江,横山隆亮,西谷和彦,南栄一,佐藤忍,岩井宏暁
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Parasitic Plant-Host Interactions Control Endoreduplication-Mediated Cell Expansion During Haustorial Development of the Holoparasitic Plant, Cuscuta Campestris2017

    • Author(s)
      Narukawa H., Kaga Y., Takeshi Kuroha T, Yokoyama R., Nishitani K.
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Quantitative Imaging Approaches to Mechanisms of Cell Wall Construction Using Arabidopsis Mesophyll Protoplasts2017

    • Author(s)
      Kuki H., Higaki T., Yokoyama R., Kuroha T, Hasezawa S., Nishitani K.
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A Model for the Molecular Mechanism of Vascular Development in the Haustorium of the Parasitic Plant Cuscuta Campestris.2017

    • Author(s)
      Kaga Y., Obayashi T., Yokoyama R., Nishitani K.
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Elucidation of the molecular mechanisms controlling flowering in the stem parasitic plant Cuscuta campestris.2017

    • Author(s)
      Kato M., Yokoyama R., Kuroha T., Abe M., Nishitani K.
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Exploring the function of cellulose end transglycosilase (CET) in plants2017

    • Author(s)
      Mikami S., Kuroha T., Shinohara N., Yokoyama R., Nishitani K.
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 浮きイネにおける洪水への適応とその進化および栽培化2017

    • Author(s)
      黒羽剛,Diane Wang,Susan R. McCouch,横山隆亮,西谷和彦,芦苅基行
    • Organizer
      東北植物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Cell Wall-Related Gene Families in Land Plants2017

    • Author(s)
      Yokoyama R., Kuroha T., Kuki H., Shinohara N., Mikami S., Nishitani K.
    • Organizer
      第65回NIBBコンファレンス
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] 農林バイオマス資源と地域利活用2018

    • Author(s)
      近藤始彦・荒井裕美子・趙鋭・西谷和彦
    • Total Pages
      419
    • Publisher
      養賢堂
    • Related Report
      2017 Annual Research Report
  • [Book] 植物生理学・発生学 第6版2017

    • Author(s)
      西谷和彦・島崎研一郎(監訳)
    • Total Pages
      832
    • Publisher
      講談社
    • Related Report
      2017 Annual Research Report
  • [Remarks] 神奈川大学理学部

    • URL

      https://www.sci.kanagawa-u.ac.jp/bio/faculty_member.html

    • Related Report
      2018 Annual Research Report
  • [Remarks] 東北大学 西谷研究室

    • URL

      http://www.biology.tohoku.ac.jp/lab-www/nishitani_lab/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 東北大学大学院生命科学研究科植物細胞壁機能分野

    • URL

      http://www.biology.tohoku.ac.jp/lab-www/nishitani_lab/

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

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