| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2017: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Outline of Final Research Achievements |
We found that not only non-structural carbohydrates but also structural ones accumulated when Arabidopsis and some legume plants were grown at high CO2. Cell walls thickened considerably by two to three folds and the mesophyll conductance measured by the carbon isotope discrimination method decreased with the increase in the cell wall thickness. Contrary to the widely-held view, starch accumulation per se did not decrease the mesophyll conductance.
Stomata are often though to move autonomously by the activity of stomatal guard cells. Here, we developed an experimental system in which the epidermal strips prepared from Commelina commmunis leaves placed on the mesophyll pre-treated in various ways. Stomatal opening in the epidermal strips was enhanced when the mesophyll segments were pre-treted at low CO2 and/or in the light. The leaf segment placed at high CO2 and/or in the dark produced negative signal(s). These mesophyll signal(s) enhanced stomatal responses.
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