Coordination of plant development by peptide hormone pathways and phytohormone pathways.

• Project/Area Number: 17H03695
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya University
• Principal Investigator: Uchida Naoyuki 名古屋大学, 遺伝子実験施設, 教授 (40467692)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2018: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: ペプチドホルモン / 低分子ホルモン / 受容体

Outline of Final Research Achievements

This study aimed to identify previously unreported phenomena regulated by the EPFL ligand family members and their receptors, ER family proteins. We also investigated plant hormone pathways that are regulated downstream of the EPFL and ER family pathway. As a result, we identified EPFL family members and ER family members that control the auxin pathway for leaf vein formation and those that control the gibberellin pathway for the robust development of floral organs under fluctuating temperatures.

Academic Significance and Societal Importance of the Research Achievements

新しいリガンドと受容体のペアの同定とその役割の解析を行い、実際に新しい機能を発見した本研究は、過去に未報告の細胞間コミュニケーションの提唱につながり、発生学上の独創的な発見となる。また、低分子性の植物ホルモン群は多様な現象に同時に影響を与え得る強い生理活性を持つことから、調和した発生・成長の各々の現象においてはその働きを局所的に必要最小限にだけ微調節する必要が生じるはずだが、本研究によりその精密調節の仕組みの一端が見えてききたことは学術的にも意義深く、植物の生産性向上を目指す応用につながることも期待される。

Research Products

• [Int'l Joint Research] テキサス大学(米国)
• [Int'l Joint Research] ハインリッヒハイリ大学(ドイツ)
• [Int'l Joint Research] テキサス大学(米国)
• [Int'l Joint Research] University of Washington(米国)
• [Journal Article] A peptide pair coordinates regular ovule initiation patterns with seed number and fruit size. (2020)
  • Author(s): Kawamoto N, Pino Del Carpio D, Hofmann A, Mizuta Y, Kurihara D, Higashiyama T, Uchida N, Torii KU, Colombo L, Groth G, Simon R
  • Journal Title: Curr. Biol. Volume: 30 Issue: 22 Pages: 4352-4361
  • DOI: 10.1016/j.cub.2020.08.050
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Induction of Multichotomous Branching by CLAVATA Peptide in Marchantia polymorpha (2020)
  • Author(s): Hirakawa Yuki、Fujimoto Toko、Ishida Sakiko、Uchida Naoyuki、Sawa Shinichiro、Kiyosue Tomohiro、Ishizaki Kimitsune、Nishihama Ryuichi、Kohchi Takayuki、Bowman John L.
  • Journal Title: Current Biology Volume: 30 Issue: 19 Pages: 3833-3840
  • DOI: 10.1016/j.cub.2020.07.016
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. (2020)
  • Author(s): Kohei Nishimura , Ryotaro Yamada , Shinya Hagihara , Rie Iwasaki , Naoyuki Uchida , Takumi Kamura , Koji Takahashi , Keiko U Torii , Tatsuo Fukagawa
  • Journal Title: Nucleic acids research Volume: 48 Issue: 18 Pages: e108-e108
  • DOI: 10.1093/nar/gkaa748
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Control of proliferation in the haploid meristem by CLE peptide signaling in Marchantia polymorpha. (2019)
  • Author(s): Hirakawa Y, Uchida N, Yamaguchi YL, Tabata R, Ishida S, Ishizaki K, Nishihama R, Kohchi T, Sawa S, Bowman JL
  • Journal Title: PLoS Genet. Volume: 7 Issue: 3 Pages: e1007997-e1007997
  • DOI: 10.1371/journal.pgen.1007997
  • NAID: 120006621164
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Stem cells within the shoot apical meristem: Identity, arrangement and communication. (2019)
  • Author(s): Uchida N and Torii KU
  • Journal Title: Cell. Mol. Life Sci. Volume: 76 Issue: 6 Pages: 1067-1080
  • DOI: 10.1007/s00018-018-2980-z
  • NAID: 120006650152
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] オーキシン応答の自在操作を実現する人工ホルモン・受容体ペアの創成 (2019)
  • Author(s): 高橋宏二，萩原 伸也，鳥居 啓子，打田 直行
  • Journal Title: 化学と生物 Volume: 57 Pages: 80-87
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A super strong engineered auxin-TIR1 pair. (2018)
  • Author(s): Yamada R, Murai K, Uchida N, Takahashi K, Iwasaki R, Tada Y, Kinoshita T, Itami K, Torii KU and Hagihara S
  • Journal Title: Plant Cell Physiol. Volume: 59 Issue: 8 Pages: 1538-1544
  • DOI: 10.1093/pcp/pcy127
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Harnessing synthetic chemistry to probe and hijack auxin signaling. (2018)
  • Author(s): Torii KU, Hagihara S, Uchida N and Takahashi K
  • Journal Title: New Phytol. Volume: 202 Issue: 2 Pages: 417-424
  • DOI: 10.1111/nph.15337
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ERECTA-family genes coordinate stem cell functions between the epidermal and internal layers of the shoot apical meristem. (2018)
  • Author(s): Kimura Y, Tasaka M, Torii KU and Uchida N
  • Journal Title: Development Volume: 145 Issue: 1
  • DOI: 10.1242/dev.156380
  • NAID: 120006473533
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair (2018)
  • Author(s): Uchida N, Takahashi K, Iwasaki R, Yamada R, Yoshimura M, Endo TA, Kimura S, Zhang H, Nomoto M, Tada Y, Kinoshita T, Itami K, Hagihara S and Torii KU
  • Journal Title: Nature Chem. Biol. Volume: 14 Issue: 3 Pages: 299-305
  • DOI: 10.1038/nchembio.2555
  • NAID: 120006473593
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 凸凹戦略によりデザインした人工オーキシンと受容体のペアにより植物のオーキシンシグナル伝達経路をハイジャックする (2018)
  • Author(s): 打田 直行、高橋 宏二、萩原 伸也、鳥居 啓子
  • Journal Title: First Author's Volume: N/A Pages: 016
  • DOI: 10.7875/first.author.2018.016
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mechanisms and strategies shaping plant peptide hormones. (2017)
  • Author(s): Hirakawa Y, Torii KU and Uchida N
  • Journal Title: Plant Cell Physiol. Volume: 8 Issue: 8 Pages: 1313-1318
  • DOI: 10.1093/pcp/pcx069
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Manipulation of plant hormone responses and its application to rapid depletion of target proteins in animal cells (2020)
  • Author(s): Uchida N
  • Organizer: 第43回日本分子生物学会年会 シンポジウム「Accelerating life sciences and drug discovery through whole-animal chemical biology」
• [Presentation] Coordination of cell layer-specific behaviors of stem cells in the shoot apical meristem (2019)
  • Author(s): Uchida N
  • Organizer: Principles of pluripotent stem cells underlying plant vitality
  • Int'l Joint Research / Invited
• [Presentation] Coordination of tissue layer-specific behaviors of stem cells in the shoot apical meristem (2019)
  • Author(s): Uchida N
  • Organizer: Cold Spring Harbor Asia Conference on Plant Cell and Development Biology
  • Int'l Joint Research / Invited
• [Presentation] 篩部で作動する地上部器官の成長・環境対応経路 (2019)
  • Author(s): 打田直行
  • Organizer: 日本植物学会第83回大会
• [Presentation] 植物のサイズ・老化の調節経路の解析とサイズ変化を誘導する化合物探索の試み (2019)
  • Author(s): 打田直行
  • Organizer: 多面的アプローチで紐解く個体スケーリング機構と健康寿命延伸への応用
  • Invited
• [Presentation] A bump-and-hole strategy toward freehand manipulation of plant hormone signaling (2019)
  • Author(s): Uchida N
  • Organizer: 日本学術振興会サンフランシスコ研究連絡センター設立１５周年記念「World Premier Research in Japan」
  • Int'l Joint Research / Invited
• [Presentation] 植物ホルモン応答の自在操作に向けた合成生物学的凸凹戦略 (2018)
  • Author(s): 打田直行, 高橋宏二, 萩原伸也, 鳥居啓子
  • Organizer: 日本植物生理学会年会第59回大会
• [Presentation] A small-compound-based approach to dissect mechanisms coordinating hypocotyl growth (2018)
  • Author(s): Murao M, Kato R, Endo H, Hagihara S, Itami K, Torii KU, Uchida N
  • Organizer: 日本植物生理学会年会第59回大会
• [Presentation] Functions of ERECTA-family receptor kinases in Arabidopsis leaf vein formation (2018)
  • Author(s): Nakashima Y, Kumamoto D, Endo H, Tasaka M, Torii KU, Uchida N
  • Organizer: 日本植物生理学会年会第59回大会
• [Presentation] ERECTA-family receptors coordinate layer-specific stem cell maintenance in the shoot apical meristem (2018)
  • Author(s): Kimura Y, Tasaka M, Torii KU, Uchida N
  • Organizer: 日本植物生理学会年会第59回大会
• [Presentation] Versatile control of growth and development by ERECTA-family receptor signaling (2017)
  • Author(s): Uchida N
  • Organizer: Plant Peptides & Receptors 2017
  • Int'l Joint Research / Invited
• [Presentation] A bump-and-hole strategy toward freehand manipulation of plant hormone signaling (2017)
  • Author(s): Uchida N
  • Organizer: IoC-IPMB-ITbM Joint Symposium on New Frontiers by Fusing Chemistry and Biology
  • Int'l Joint Research / Invited