Elucidation of the cell fate regulation by cell-cell communication in plant embryogenesis
Project/Area Number |
17H03697
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | Nagoya University |
Principal Investigator |
Kurihara Daisuke 名古屋大学, トランスフォーマティブ生命分子研究所, 特任講師 (90609439)
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Project Period (FY) |
2017-04-01 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2017: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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Keywords | 植物 / 胚発生 / 細胞運命 / 細胞間コミュニケーション / イメージング |
Outline of Final Research Achievements |
Embryogenesis is a fundamental process in the construction of the complex structures and functions of higher organisms from a single cell, the zygote. The purpose of this study was to clarify the molecular entities of cell-cell communication involved in the regulation of cell fate during embryogenesis. We discovered a candidate mutant as a result of comprehensive analysis focusing on ligand-receptor pairs as signaling pathways that transport outside the cell. We collected data to identify and analyze the causative genes. We also tried to establish the optical manipulation technique for analyzing the signal pathway transported in the cell and clarified the problems and improvement points.
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Academic Significance and Societal Importance of the Research Achievements |
細胞運命転換は、植物の多能性、分化可塑性を担う重要なメカニズムである。しかしながら、植物は生きたままの観察・解析の困難さから、多数の細胞が混在する組織レベルの研究に終始しており、個々の細胞で、どのように細胞運命が変化しているのかさえ、多くは未知のままである。本研究では、頂端細胞と基部細胞というわずか2細胞の発生時期において、細胞間コミュニケーションの解析を行うことができ、細胞運命転換をする様子を追跡できるため、1細胞レベルでシグナル分子のやりとり、また細胞運命転換のメカニズムに迫った。本研究成果は胚発生だけに留まらず、植物の分化可塑性を担う普遍的なメカニズム解明に繋がると期待される。
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Report
(4 results)
Research Products
(16 results)
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[Presentation] Live-cell imaging of the axis formation in Arabidopsis zygote2018
Author(s)
Minako Ueda, Yusuke Kimata, Takehide Kato, Takumi Higaki, Daisuke Kurihara, Tomomi Yamada, Shoji Segami, Miyo Terao Morita, Masayoshi Maeshima, Seiichiro Hasezawa, Tetsuya Higashiyama, Masao Tasaka, Naoe Ando
Organizer
the 25th International Congress on Sexual Plant Reproduction
Related Report
Int'l Joint Research / Invited
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