| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2019: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Outline of Final Research Achievements |
PHS1 (Propyzamide-Hypersensitive 1) has evolved in some green algae and land plants, and is a hybrid enzyme composed of a tubulin-kinase domain and a regulatory MAP kinase phosphatase-like domain. Hyperosmotic stress rapidly activates its tubulin kinase. In this study, in vitro reconstitution experiments with recombinant PHS1 proteins were used to demonstrate functional roles of several candidate amino acid residues that were postulated to be involved in the auto-activation mechanisms. In addition, characterization of liverwort knockout mutants of three MAP kinase genes demonstrated that MAP kinases are not necessary for stress-induced activation of PHS1.
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