Microtubule-mediated responses to environmental stress
| Project/Area Number |
17H03698
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
Hashimoto Takashi 奈良先端科学技術大学院大学, 先端科学技術研究科, 教授 (80180826)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2019: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Keywords | 植物 / 微小管 / 乾燥ストレス / チューブリン / リン酸化 / MAPキナーゼ / 浸透圧ストレス / ゼニゴケ / ストレス応答 / 乾燥 / ストレス / シロイヌナズナ / 活性化 / シグナル伝達 / 環境応答 / 細胞骨格 |
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| Outline of Final Research Achievements |
PHS1 (Propyzamide-Hypersensitive 1) has evolved in some green algae and land plants, and is a hybrid enzyme composed of a tubulin-kinase domain and a regulatory MAP kinase phosphatase-like domain. Hyperosmotic stress rapidly activates its tubulin kinase. In this study, in vitro reconstitution experiments with recombinant PHS1 proteins were used to demonstrate functional roles of several candidate amino acid residues that were postulated to be involved in the auto-activation mechanisms. In addition, characterization of liverwort knockout mutants of three MAP kinase genes demonstrated that MAP kinases are not necessary for stress-induced activation of PHS1.
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| Academic Significance and Societal Importance of the Research Achievements |
植物は環境変動や生物的ストレスに対して生育プログラムや代謝を恒常的に適応させている。環境ストレス応答は植物ホルモンやグローバルな遺伝子発現の変化をもたらすが、細胞骨格が関与するストレス応答の初期反応の分子機構は未解明の部分が多い。本研究成果はマイルドな乾燥ストレスが微小管再編成をもたらす分子機構に新たな知見をもたらしたものであり、作物や有用植物の乾燥適応戦略を深く理解し、乾燥ストレス下における高収量品種の育種につながると期待される。
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Modification of growth anisotropy and cortical microtubule dynamics in Arabidopsis hypocotyls grown under microgravity conditions in space2017
Author(s)
Soga K, Yamazaki C, Kamada M, Tanigawa N, Kasahara H, Yano S, Kojo KH, Kutsuna N, Kato T, Hashimoto T, Kotake T, Wakabayashi K, Hoson T
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Journal Title
Phyiologia Plantarum
Volume: 162
Issue: 1
Pages: 135-144
DOI
Related Report
Peer Reviewed
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