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Integrative studies on the processive degradation of macromolecules in chloroplasts

Research Project

Project/Area Number 17H03699
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionOkayama University

Principal Investigator

SAKAMOTO Wataru  岡山大学, 資源植物科学研究所, 教授 (20222002)

Co-Investigator(Kenkyū-buntansha) 高見 常明  岡山大学, 資源植物科学研究所, 技術職員 (70614254)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywords植物生理 / オルガネラ分化 / プロテアーゼ / ヌクレアーゼ / 葉緑体 / 光合成 / トランスポーター / 葉緑体分化 / タンパク質リン酸化
Outline of Final Research Achievements

Chloroplasts in land plants, which originate from endosymbiosis of cyanobacteria, are the organelles that develop thylakoid membranes in response to light and are responsible not only for the synthesis of photosynthates but also for the synthesis of various compounds like lipids, amino acids and phytohormones. As a site of light energy conversion, chloroplasts are susceptible to damage caused by excess light, and the quality control and environmental adaptation functions related to chloroplast maintenance also have a significant impact on plant growth. Our previous studies have revealed the importance of the processive degradation of macromolecules such as proteins and nucleic acids for chloroplast homeostasis. In this study, we conducted genetic and physiological studies to understand the new functions of chloroplasts through these processive degradation mechanisms.

Academic Significance and Societal Importance of the Research Achievements

光エネルギーを利用して大気や海中の二酸化炭素を有機物に変換する光合成は、我々にとって最も重要な生体反応の1つである。陸上植物では、光合成はシアノバクテリアの細胞内共生に由来する「葉緑体」という植物細胞のオルガネラで行われている。葉緑体は光阻害を受けやすく、それらを維持するためのメカニズムを分子レベルで解明することは、葉緑体機能の強化と光に強い作物の育成につながる。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (54 results)

All 2021 2020 2019 2018 2017 Other

All Int'l Joint Research (6 results) Journal Article (16 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 16 results,  Open Access: 9 results) Presentation (28 results) (of which Int'l Joint Research: 15 results,  Invited: 10 results) Remarks (4 results)

  • [Int'l Joint Research] National Center for Scientific Research/Institut de Biologie Physico-Chimique(フランス)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] hinese Academy of Sciences/Shanghai Center for Plant Stress Science(中国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Munster/Inst of Plant Biology and Biotechnology(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] CNRS-IBPC(フランス)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Chinese Academy of Sciences(中国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Chinese Academy of Sciences/Shanghai Center for Plant Stress Biology(中国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Barley albino lemma 1 resulted from mutations in a Golden2-like gene reduces seed weight2021

    • Author(s)
      Shin Taketa 1 , Momoko Hattori 1 , Tsuneaki Takami 1 , Eiko Himi 1 , Wataru Sakamoto 1
    • Journal Title

      Plant And Cell Physiology

      Volume: 62 Issue: 3 Pages: 447-457

    • DOI

      10.1093/pcp/pcab001

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RAD-seq-Based High-Density Linkage Map Construction and QTL Mapping of Biomass-Related Traits in Sorghum using the Japanese Landrace Takakibi NOG.2020

    • Author(s)
      Kajiya-Kanegae H, Takanashi H, Fujimoto M, Ishimori M, Ohnishi N, Wacera W F, Omollo EA, Kobayashi M, Yano K, Nakano M, Kozuka T, Kusaba M, Iwata H, Tsutsumi N, Sakamoto W
    • Journal Title

      Plant Cell Physiol.

      Volume: 61 Issue: 7 Pages: 1262-1272

    • DOI

      10.1093/pcp/pcaa056

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phos-tag-based approach to study protein phosphorylation in the thylakoid membrane2020

    • Author(s)
      Nishioka Keiji、Kato Yusuke、Ozawa Shin-ichiro、Takahashi Yuichiro、Sakamoto Wataru
    • Journal Title

      Photosynthesis Research

      Volume: 147 Issue: 1 Pages: 107-124

    • DOI

      10.1007/s11120-020-00803-1

    • NAID

      120006953502

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phototropin‐ and photosynthesis‐dependent mitochondrial positioning in Arabidopsis thaliana mesophyll cells2020

    • Author(s)
      Islam Md Sayeedul、Van Nguyen Toan、Sakamoto Wataru、Takagi Shingo
    • Journal Title

      Journal of Integrative Plant Biology

      Volume: 62 Issue: 9 Pages: 1352-1371

    • DOI

      10.1111/jipb.12910

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Degradation of chloroplast DNAs as an efficient strategy for phosphorus recycling in seed plants2019

    • Author(s)
      坂本 亘、高見 常明
    • Journal Title

      生化学

      Volume: 91 Issue: 6 Pages: 785-789

    • DOI

      10.14952/SEIKAGAKU.2019.910785

    • NAID

      40022117251

    • ISSN
      0037-1017
    • Year and Date
      2019-12-25
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Contribution of Organelle DNA Degradation to Nutrient Recycling: Adaptation Mechanism of Seed Plants Derived from Endosymbiosis2019

    • Author(s)
      坂本亘、高見常明
    • Journal Title

      KAGAKU TO SEIBUTSU

      Volume: 57 Issue: 8 Pages: 478-483

    • DOI

      10.1271/kagakutoseibutsu.57.478

    • NAID

      130007883278

    • ISSN
      0453-073X, 1883-6852
    • Year and Date
      2019-08-01
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation of the Chloroplastic Metalloprotease FtsH in Arabidopsis Characterized by Phos-Tag SDS-PAGE.2019

    • Author(s)
      Kato Y, Sakamoto W.
    • Journal Title

      Front Plant Sci.

      Volume: 10 Pages: 1080-1080

    • DOI

      10.3389/fpls.2019.01080

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Photosynthetic Responses to High Temperature and Strong Light Suggest Potential Post-flowering Drought Tolerance of Sorghum Japanese Landrace Takakibi2019

    • Author(s)
      Ohnishi Norikazu、Wacera W. Fiona、Sakamoto Wataru
    • Journal Title

      Plant and Cell Physiology

      Volume: 60 Issue: 9 Pages: 2086-2099

    • DOI

      10.1093/pcp/pcz107

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impaired PSII proteostasis promotes retrograde signaling via salicylic acid.2019

    • Author(s)
      Duan J, Lee KP, Dogra V, Zhang S, Liu K, Caceres-Moreno C, Lv S, Xing W, Kato Y, Sakamoto W, Liu R, Macho AP, Kim C.
    • Journal Title

      Plant Physiol.

      Volume: 180 Issue: 4 Pages: 2182-2197

    • DOI

      10.1104/pp.19.00483

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Organelle DNA degradation contributes to the efficient use of phosphate in seed plants.2018

    • Author(s)
      Takami T. Ohnishi N. Kurita Y, Iwamura S, Ohnishi M. Kusaba M. Mimura T. and Sakamoto W.
    • Journal Title

      Nature Plants

      Volume: 4 Issue: 12 Pages: 1044-1055

    • DOI

      10.1038/s41477-018-0291-x

    • NAID

      120006627286

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Photosystem II Repair Cycle Requires FtsH Turnover through the EngA GTPase2018

    • Author(s)
      Kato, Y., Hyodo, K., Sakamoto, W.
    • Journal Title

      Plant Physiol.

      Volume: 178 Issue: 2 Pages: 596-611

    • DOI

      10.1104/pp.18.00652

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] FtsH protease in the thylakoid membrane: physiological functions and the regulation of protease activity2018

    • Author(s)
      Kato, Y. and Sakamoto, W.
    • Journal Title

      Frontiers Plant Sci.

      Volume: 9 Pages: 855-855

    • DOI

      10.3389/fpls.2018.00855

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chloroplast DNA dynamics: copy number, quality control, and degradation.2018

    • Author(s)
      Sakamoto W. and Takami T.
    • Journal Title

      Plant and Cell Physiology

      Volume: 59 Issue: 6 Pages: 1120-1127

    • DOI

      10.1093/pcp/pcy084

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] VIPP1 involved in chloroplast membrane integrity has GTPase activity in vitro.2018

    • Author(s)
      Ohnishi N. Zhang L. and Sakamoto W.
    • Journal Title

      Plant Physiology

      Volume: 177 Issue: 1 Pages: 328-338

    • DOI

      10.1104/pp.18.00145

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Meeting Report - Taiwan - Japan Plant Biology 2017 Spotlight Issue: From light signal to photosynthesis and chloroplast development2018

    • Author(s)
      Nishimura, K., Matsushita, T., Shikanai, T., and Sakamoto, W.
    • Journal Title

      Plant and Cell Physiology

      Volume: 印刷中 Issue: 6 Pages: 1099-1103

    • DOI

      10.1093/pcp/pcy077

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The Rubisco Chaperone BSD2 May Regulate Chloroplast Coverage in Maize Bundle Sheath Cells.2017

    • Author(s)
      Salesse C, Sharwood R, and Sakamoto W, Stern D.
    • Journal Title

      Plant Physiology

      Volume: 175(4) Issue: 4 Pages: 1624-1633

    • DOI

      10.1104/pp.17.01346

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] プロトン駆動力を維持するチラコイド 膜リモデリングのVIPP1による理解2021

    • Author(s)
      坂本亘、大西紀和
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 葉緑体膜の形成と機能維持に重要なVIPP1が示す新奇ATPaseおよびGTPase活性の異なる特徴2021

    • Author(s)
      大西紀和、杉本学、張林剛、坂本亘
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] dpd1変異はオートファジー変異体の早枯れ表現型を抑制する2021

    • Author(s)
      高見常明、坂本亘
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 緑藻クラミドモナスの外縁LHCI欠損による光捕集複合体再構成能力の生化学的解析2021

    • Author(s)
      小澤真一郎、Philipp Gabelein, Felix Buchert, Laura Mosebach, Susan Hawat, Martin Scholz, 坂本亘、Michael Hippler
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Photo-oxidative damage of Photosystem II and specific degradation of D1 reaction center protein by FtsH protease.2019

    • Author(s)
      Sakamoto W.
    • Organizer
      The 9th Asia-Oceania Conference on Photobiology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Degradation of photo-damaged D1 in the PSII repair and FtsH.2019

    • Author(s)
      Kato, Y. and Sakamoto, W.
    • Organizer
      Japan-America Seminar
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Inhibition and protection of photosynthetic components in chloroplasts.2019

    • Author(s)
      Sakamoto, W.
    • Organizer
      2019 Taiwan Society of Plant Biologists Annual Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] VIPP1 Protein and its Possible Role in Chloroplasts.2019

    • Author(s)
      Sakamoto, W.
    • Organizer
      Biogenesis of Thylakoid Membranes: Spatiotemporal Organization of Photosynthetic Protein Complex Assembly, 2nd International Meeting DFG, Research Unit FOR2092
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] D1タンパク質の酸化修飾がFtsHによる基質認識につながる可能性.2019

    • Author(s)
      加藤裕介・Vivek Dogra・Mingyue Li・黒田洋詩・高橋裕一郎・斉藤圭亮・石北 央・Chanhong Kim・坂本 亘
    • Organizer
      第10回日本光合成学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 葉緑体膜の形成および機能維持に重要なVIPP1が示す新奇ATPase活性の解析2019

    • Author(s)
      大西紀和、張林剛、坂本亘
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] var2変異体の斑入りセクタートランスクリプトームデータの再検討2019

    • Author(s)
      高見常明、坂本亘
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Possible role of protein degradation as a signaling mechanism in chloroplasts2018

    • Author(s)
      Sakamoto W.
    • Organizer
      1st Asia-Oceania International Congress on Photosynthesis
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Regulation of FtsH function and proper FtsH turnover in the PSII repair cycle.2018

    • Author(s)
      Kato, Y., Hyodo, K., and Sakamoto, W.
    • Organizer
      1st Asia-Oceania International Congress on Photosynthesis
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Chloroplast DNA degradation serving as nutrient reservoir in plants.2018

    • Author(s)
      Sakamoto, W. and Takami, T.
    • Organizer
      Japan-Finland Seminar 2018-Shaping photosynthesis against climate change and toward efficient water and nutrient management.
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Chloroplast protein degradation and beyond: DtsH and a possible peptide export.2018

    • Author(s)
      Sakamoto, W., Nishimura K., and Kato, Y.
    • Organizer
      International Symposium on Photosynthesis and Chloroplast Biogenesis
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Contribution of chloroplast DNA degradation by DPD1 exonuclease under phosphate deficient condition2018

    • Author(s)
      Takami T. and Sakamoto W.
    • Organizer
      International Symposium on Photosynthesis and Chloroplast Biogenesis
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of oxidative post-translational modification in PSII repair2018

    • Author(s)
      Kato, Y., DOgra, V., Li, M., Kuroda, H., Takahashi, Y., Kim, C., and Sakamoto, W.
    • Organizer
      International Symposium on Photosynthesis and Chloroplast Biogenesis
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] VIPP1, the membrane integrity-maintaining protein in chloroplasts, has GTPase activity in vitro2018

    • Author(s)
      Ohnishi, N., Zhang, L., and Sakamoto, W.
    • Organizer
      International Symposium on Photosynthesis and Chloroplast Biogenesis
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ソルガムの在来種たかきびが出穂後に示す高温・強光耐性の光合成活性による評価2018

    • Author(s)
      大西紀和、坂本亘
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Phos-tagを用いたチラコイド膜におけるリン酸化タンパク質の網羅的検出法2018

    • Author(s)
      西岡佳司、加藤裕介、小澤真一郎、高橋雄一郎、坂本 亘
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 光化学系II修復サイクルでのFtsHプロテアーゼ自身の品質管理の重要性2018

    • Author(s)
      加藤裕介、兵頭究、坂本 亘
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 葉緑体DNA分解を介したリン利用効率・分配の最適化2018

    • Author(s)
      高見 常明, 大西 紀和, 栗田 悠子, 岩村 青子, 大西 美輪, 三村 徹郎 坂本 亘
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シロイヌナズナ葉緑体ペプチドエクスポーターの解析2018

    • Author(s)
      西村健司、関谷堂真、石森元幸、高見常明、加藤裕介、宮地孝明、坂本亘
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] VIPP1, a chloroplast membrane integrity-maintaining protein, has GTP-binding and hydrolysis activities in vitro2017

    • Author(s)
      Norikazu Ohnishi, Lingang Zhang and Wataru Sakamoto
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Possible regulation mechanisms of chloroplastic FtsH metalloprotease by protein phosphorylation.2017

    • Author(s)
      Kato Y, Sakamoto W.
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Chloroplast DNA and nutrient salvage: a new concept.2017

    • Author(s)
      Sakamoto, W., Ohnishi, N., Takami, T.
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Comprehensive analysis of phosphoprotein in thylakoid membranes.2017

    • Author(s)
      Nishioka,K., Kato, Y., Ozawa, S., Takahashi, Y., Sakamoto, W.
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Nuclear transcriptome rewiring involving a peptide-exporting ABC transporter on chloroplast envelopes.2017

    • Author(s)
      Nishimura, K., Ishimori, M., Sekiya, T., Watson, S., Takami, T., Miyaji, T., Sakamoto, W.
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 光合成の機能維持にはタンパク質分解酵素自身の品質管理が重要

    • URL

      http://www.rib.okayama-u.ac.jp/researchactivity/20181213-1.html

    • Related Report
      2018 Annual Research Report
  • [Remarks] オルガネラDNAを自己分解してリン栄養分にする生命現象の発見

    • URL

      http://www.rib.okayama-u.ac.jp/researchactivity/20181119-1.html

    • Related Report
      2018 Annual Research Report
  • [Remarks] 葉緑体DNAダイナミクスの総説

    • URL

      http://www.rib.okayama-u.ac.jp/researchactivity/20180628-2.html

    • Related Report
      2018 Annual Research Report
  • [Remarks] 葉緑体の機能維持に重要な新奇GTPaseの発見

    • URL

      http://www.rib.okayama-u.ac.jp/researchactivity/20180521-1.html

    • Related Report
      2018 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2022-01-27  

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