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Plastid peptidoglycan in streptophyte plants

Research Project

Project/Area Number 17H03701
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionKumamoto University

Principal Investigator

Takano Hiroyoshi  熊本大学, 大学院先端科学研究部(理), 教授 (70242104)

Co-Investigator(Kenkyū-buntansha) 児島 征司  東北大学, 学際科学フロンティア研究所, 助教 (20745111)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2018: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywords葉緑体 / バイオテクノロジー / 植物 / 進化
Outline of Final Research Achievements

The acquisition of chloroplasts was an important event in the evolution of plant cells. Although the plastids of green plants are thought to have lost the ability to produce peptidoglycan (PG) during evolution, we found that the moss Physcomitrella (Physcomitrium patens) use PG for chloroplast division. In this study, structural characterization of moss PG was analyzed. We identified new homologs of bacterial PG genes such as MurJ and MltB to show their relation to moss chloroplast division. Moreover, we visualized chloroplast PG in the charophyte alga Klebsormidium nitens, revealed the presence of peptidoglycan in the intermembrane space of the moss chloroplast envelope, showed diverse origins of enzymes involved in the chloroplast PG synthesis, and analyzed MurE from the gymnosperm Larix gmelinii.

Academic Significance and Societal Importance of the Research Achievements

我々の発見した、ストレプト植物における「葉緑体型PG」を研究することが本研究の一番の特色である。我々の研究により、これらの細菌由来のシステムが葉緑体分裂や分化といった植物細胞の基本システムとかかわることが明らかとなってきた。植物のエネルギー産生オルガネラである葉緑体の分裂機構を研究することは、物質生産にも関わる重要なテーマである。葉緑体分裂は、PGを持たないシロイヌナズナや紅藻シアニディオシゾンで精力的に研究されてきたため、PGを持つコケの葉緑体分裂機構の解析は、基部ストレプト植物における一般性とともに、葉緑体分裂機構の進化に迫ることができる研究である。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (36 results)

All 2021 2020 2019 2018 2017 Other

All Int'l Joint Research (3 results) Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (24 results) (of which Int'l Joint Research: 10 results,  Invited: 4 results)

  • [Int'l Joint Research] 内蒙古大学(中国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] 内蒙古大学(中国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] 内蒙古大学(中国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Genes encoding lipid II flippase MurJ and peptidoglycan hydrolases are required for chloroplast division in the moss Physcomitrella patens.2021

    • Author(s)
      Utsunomiya, H., Saiki, N., Kadoguchi, H., Fukudome, M., Hashimoto, S., Ueda, M., Takechi, K. and Takano, H.
    • Journal Title

      Plant Molecular Biology

      Volume: in press Issue: 4-5 Pages: 405-415

    • DOI

      10.1007/s11103-020-01081-0

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two atypical ANGUSTIFOLIA without a plant‐specific C‐terminus regulate gametophore and sporophyte shapes in the moss Physcomitrium ( Physcomitrella ) patens2021

    • Author(s)
      Takechi Katsuaki、Nagase Hiroaki、Furuya Tomoyuki、Hattori Koro、Sato Yoshikatsu、Miyajima Kensuke、Higuchi Tomofumi、Matsuda Ryuya、Takio Susumu、Tsukaya Hirokazu、Takano Hiroyoshi
    • Journal Title

      The Plant Journal

      Volume: 105 Issue: 5 Pages: 1390-1399

    • DOI

      10.1111/tpj.15121

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 葉緑体「壁」を持つストレプト植物の葉緑体分裂機構 - Invisible wallを求めて -2021

    • Author(s)
      高野 博嘉
    • Journal Title

      Plant Morph.

      Volume: in press

    • NAID

      40022708324

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two ANGUSTIFOLIA genes regulate gametophore and sporophyte development in Physcomitrella patens2020

    • Author(s)
      Hashida Yoshikazu、Takechi Katsuaki、Abiru Tomomi、Yabe Noriyuki、Nagase Hiroaki、Hattori Koro、Takio Susumu、Sato Yoshikatsu、Hasebe Mitsuyasu、Tsukaya Hirokazu、Takano Hiroyoshi
    • Journal Title

      The Plant Journal

      Volume: 101 Issue: 6 Pages: 1318-1330

    • DOI

      10.1111/tpj.14592

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Visualization of Plastid Peptidoglycan in the Charophyte Alga <i>Klebsormidium nitens</i> Using a Metabolic Labeling Method2018

    • Author(s)
      Takano Hiroyoshi、Tsunefuka Takashi、Takio Susumu、Ishikawa Hayato、Takechi Katsuaki
    • Journal Title

      CYTOLOGIA

      Volume: 83 Issue: 4 Pages: 375-380

    • DOI

      10.1508/cytologia.83.375

    • NAID

      130007552949

    • ISSN
      0011-4545, 1348-7019
    • Year and Date
      2018-12-25
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thylakoid membranes contain a non-selective channel permeable to small organic molecules2018

    • Author(s)
      Kojima S, Iwamoto M, Oiki S, Tochigi S, Takahashi H
    • Journal Title

      Journal of Biological Chemistry

      Volume: 印刷中 Issue: 20 Pages: 7777-7785

    • DOI

      10.1074/jbc.ra118.002367

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single-Pixel Densitometry Revealed the Presence of Peptidoglycan in the Intermembrane Space of the Moss Chloroplast Envelope in Conventional Electron Micrographs2017

    • Author(s)
      Sato, N., Toyoshima, M., Tajima, N., Takechi, K. and Takano, H.
    • Journal Title

      Plant and Cell Physiology

      Volume: 58 Issue: 10 Pages: 1743-1751

    • DOI

      10.1093/pcp/pcx113

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genes Sufficient for Synthesizing Peptidoglycan Are Retained in Gymnosperm Genomes, and MurE from Larix gmelinii Can Rescue the Albino Phenotype of Arabidopsis MurE.2017

    • Author(s)
      Lin X, Li N, Kudo H, Zhang Z, Li J, Wang L, Zhang W, Takechi K, Takano H.
    • Journal Title

      Plant and Cell Physiology

      Volume: 58 Issue: 3 Pages: 587-897

    • DOI

      10.1093/pcp/pcx005

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Diverse origins of enzymes involved in the biosynthesis of chloroplast peptidoglycan2017

    • Author(s)
      Sato, N., Takano, H.
    • Journal Title

      Journal of Plant Research

      Volume: 130 Issue: 4 Pages: 635-645

    • DOI

      10.1007/s10265-017-0935-3

    • NAID

      40021237097

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] Invisible wall を追う ― 基部ストレプト植物の葉緑体に存在するペプチドグリカン壁 ―2020

    • Author(s)
      高野 博嘉
    • Organizer
      日本植物形態学会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] AP2/ERF transcription factors regulate salt induced chloroplast division in the moss, Physcomitrella patens.2020

    • Author(s)
      Do, T.H., Prapapron, P., Menaka, A., Takano, H., Yoshioka, Y., Teh O.-K., Fujita, T.
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Moss chloroplast division with peptidoglycan2019

    • Author(s)
      Takano, H.
    • Organizer
      EMBO Workshop “Bacterial cell division: Closing the gap”
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Evolution of MurE functions from moss plastid division to angiosperm chloroplast development2019

    • Author(s)
      Kajisa, I, Lin, X., E, Y., Kudo, H., Takio, S., Takechi, K. and Takano, H.
    • Organizer
      iMoss2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evolutionarily distinct pathways are present to regulate chloroplast proliferation in the moss, Physcomitrella patens2019

    • Author(s)
      Fujita, T., Pongthai, P., Do, H. T., Inoue, N., Yoshioka, Y. and Takano, H.
    • Organizer
      iMoss2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 変異植物体が巨大葉緑体形質を示すMurEとアルビノ形質を示すMurEの相同性と変異領域2019

    • Author(s)
      加治佐一朗、林暁飛、鄂一嵐、工藤裕美、滝尾進、武智克彰、高野博嘉
    • Organizer
      日本植物形態学会第31回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 葉緑体分裂に関与するMurEと葉緑体分化に関与するMurEの相同性と変異領域2019

    • Author(s)
      加治佐一朗、林暁飛、鄂一嵐、工藤裕美、池田孝介、滝尾進、武智克彰、高野博嘉
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 環境に応じて使い分けられるコケ植物葉緑体の増殖方法2019

    • Author(s)
      藤田知道、Prapaporn Pongthai、Huong Thi Do、井上夏実、吉岡泰、高野博嘉
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] コケで葉緑体分裂に、被子植物で葉緑体分化に関与するMurEのアミノ酸配列比較と機能相補解析2019

    • Author(s)
      加治佐一朗、林暁飛、鄂一嵐、工藤裕美、滝尾進、武智克彰、高野博嘉
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Evolution of plastids with peptidoglycan wall2018

    • Author(s)
      Takano, H.
    • Organizer
      Plant Science Seminar in Fac. Sci, Hokkaido Univ.
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] コケで葉緑体ペプチドグリカン合成に、シロイヌナズナで葉緑体遺伝子発現に関わるMurEの機能相補解析2018

    • Author(s)
      加治佐一朗、林暁飛、鄂一嵐、池田孝介、工藤裕美、滝尾進、武智克彰、高野博嘉
    • Organizer
      日本植物形態学会第30回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 緑色植物に保存された、葉緑体の分化や分裂に関与するMurEの機能相補実験2018

    • Author(s)
      加治佐一朗、林暁飛、鄂一嵐、工藤裕美、池田孝介、滝尾進、武智克彰、高野博嘉
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Moss chloroplasts are surrounded by a peptidoglycan wall.2017

    • Author(s)
      Takano, H., Tadano, S., Ishikawa, H., Takio, S. and Takechi, K.
    • Organizer
      Plant Biology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Chloroplasts in Physcomitrella patens are surrounded by a peptidoglycan wall.2017

    • Author(s)
      Hirano, T., Tanidokoro, K., Tadano, S., Ishikawa, H., Takio, S., Takechi, K. and Takano, H.
    • Organizer
      iMoss2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Moss plastids have peptidoglycan wall, similar to cyanobacteria2017

    • Author(s)
      Takano, H.
    • Organizer
      1st IROAST Symposium “Plant Cell and Developmental Biology: Approaches to Multiscale Biosystems”
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Cross-species complementation assay with MurE genes that are related to chloroplast division in bryophytes and that are affected chloroplast development in seed plants2017

    • Author(s)
      Kodo, H., Lin, X., Susumu, T., Takechi, K., Takano, H.
    • Organizer
      1st IROAST Symposium “Plant Cell and Developmental Biology: Approaches to Multiscale Biosystems”
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Structural analysis of moss plastid peptidoglycan2017

    • Author(s)
      Kishimoto, K., Kojima, S., Mori, H., Susumu, T., Takechi, K., Takano, H.
    • Organizer
      1st IROAST Symposium “Plant Cell and Developmental Biology: Approaches to Multiscale Biosystems”
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cross-species complementation assay of macrochloroplast phenotype in moss murE mutant and of albino phenotype in Arabidopsis ones with larch MurE gene2017

    • Author(s)
      Kodo, H., Lin, X., Susumu, T., Takechi, K., Takano, H.
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Structural analysis of moss plastid peptidoglycan using a metabolic labeling method with click chemistry2017

    • Author(s)
      Kishimoto, K., Kojima, S., Mori, H., Susumu, T., Takechi, K., Takano, H.
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 葉緑体に局在するLysMドメイン含有タンパク質のヒメツリガネゴケ遺伝子破壊ラインを用いた解析2017

    • Author(s)
      橋元 恵里奈、松下 祐美、森 仁志、瀧尾 進、武智 克彰、高野 博嘉
    • Organizer
      第29回日本植物形態学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] コケで巨大葉緑体形質を示し、シロイヌナズナでアルビノの植物体となるMurE遺伝子破壊ラインのカラマツMurEも用いた種間相補解析2017

    • Author(s)
      工藤 裕美、林 飛暁、瀧尾 進、武智 克彰、高野 博嘉
    • Organizer
      第29回日本植物形態学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヒメツリガネゴケの葉緑体に局在するLysMドメイン含有タンパク質の機能解析2017

    • Author(s)
      橋元 恵里奈、松下 祐美、森 仁志、瀧尾 進、武智 克彰、高野 博嘉
    • Organizer
      第81回日本植物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] コケで葉緑体分裂に、シロイヌナズナで葉緑体分化に関するMurEとカラマツMurEを用いた種間相補解析2017

    • Author(s)
      工藤 裕美、林 飛暁、瀧尾 進、武智 克彰、高野 博嘉
    • Organizer
      第81回日本植物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヒメツリガネゴケゲノム中に存在する2種のMurE遺伝子の葉緑体分裂・分化における機能2017

    • Author(s)
      池田孝介、工藤裕美、加治佐一朗、滝尾進、武智克彰,高野博嘉
    • Organizer
      第59回日本植物生理学会
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2022-01-27  

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