New interactome strategy elucidating the fertilization mechanism of malaria parasite
| Project/Area Number |
17H03707
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Morphology/Structure
|
|---|
| Research Institution | Osaka University (2020)
Juntendo University (2017-2019) |
|---|
Principal Investigator |
Mori Toshiyuki 大阪大学, 微生物病研究所, 助教 (00462739)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
平井 誠 順天堂大学, 医学部, 准教授 (50326849)
|
|---|
| Project Period (FY) |
2017-04-01 – 2021-03-31
|
| Project Status |
Completed (Fiscal Year 2020)
|
| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
|
|---|
| Keywords | 受精 / 有性生殖 / 膜タンパク質 / 細胞間相互作用 / 配偶子 / GCS1 / ネズミマラリア原虫 / 配偶子融合 / マラリア原虫 / 膜融合 / 受精因子 / マラリア / 媒介蚊 / インタラクトーム |
|---|
| Outline of Final Research Achievements |
We have tried to identify novel factors interacting with GCS1, the male-side gamete fusion factor conserved among animals, plants and protists, using the rodent malaria parasite Plasmodium berghei. We succeeded in producing parasites expressing GCS1 conjugated with BioID2, which was recently developed as a proximity-dependent biotin ligase. Although we have not been able to detect female-side partners of GCS1, our findings suggest a promising possibility to identify fertilization factors.
|
| Academic Significance and Societal Importance of the Research Achievements |
マラリアは蚊によって媒介される感染症であり、蚊の体内ではマラリア原虫が有性生殖によって増殖する。このことから、マラリア原虫の受精を妨げることがマラリア撲滅の戦略の一つとして現在注目されており、受精分子機構の解明が待たれている。本研究成果であるビオチン化酵素によるスクリーニング法は、マラリア原虫の受精に働く因子の効率的探索に大いに貢献するものであり、さらなる改善を加えることでマラリア原虫の受精機構解明に資する成果が期待できる。
|
Report
(5 results)
Research Products
(17 results)
-
-
-
-
-
-
-
-
[Journal Article] Lack of significant recovery of chloroquine sensitivity in Plasmodium falciparum parasites following discontinuance of chloroquine use in Papua New Guinea2018
Author(s)
Sekihara M, Tachibana S-I, Yamauchi M, Yatsushiro S, Tiwara S, Fukuda N, Ikeda M, Mori T, Hirai M, Hombhanje F, Mita T
-
Journal Title
Malaria Journal
Volume: 17
Issue: 1
Pages: 434-434
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
[Journal Article] The male gamete membrane protein DMP9/DAU2 is required for double fertilization in flowering plants.2018
Author(s)
Takahashi T., Mori, T., Ueda, K., Yamada, L., Nagahara, S., Higashiyama, T., Sawada, H., Igawa, T.
-
Journal Title
Development
Volume: 145(23)
Issue: 23
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
