| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2017: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Outline of Final Research Achievements |
DNA double strand breaks (DSBs) cause various chromosomal rearrangements, leading to diseases such as cancer and cell senescence. On the other hand, DSB formation by Spo11 during meiosis occurs at recombination hotspots, but chromosomal translocations are suppressed, resulting in conservative genomic alterations. Application of TAQing system, a gross genome rearrangement-inducing system, to yeast cells in mitosis and meiosis revealed distinct effects on genome rearrangements. While rare translocations and copy number fluctuations occur during meiosis, they are often detected in TAQed mitotic cells. The spo11 deletion strain TAQed in meiosis failed to complete normal segregation of homologous chromosomes, suggesting that DNA cleavage by Spo11 was more important than expected.
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