| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2019: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Outline of Final Research Achievements |
In this study, we focused on Clr4 methyltransferase complex (CLRC) in fission yeast, and investigated a functional link between ubiquitin modification and heterochromatin assembly. In our previous studies, we demonstrated that CLRC possesses ubiquitylation activity on histone H3 and that H3 ubiquitylation promotes Clr4's methyltransferase activity. We further examined the molecular mechanisms underlying these events and specifically demonstrated that 1) Clr4 N-terminal regions recognize ubiquitylated H3, 2) CD-adjacent N-terminal region plays an important role in Clr4's function, 3) Clr4 interacts with the CLRC through its C-terminal region.
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