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Symbiotic genetic network: its regulatory mechanism and the evolution

Research Project

Project/Area Number 17H03717
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Evolutionary biology
Research InstitutionNational Institute for Basic Biology

Principal Investigator

Shigenobu Shuji  基礎生物学研究所, 新規モデル生物開発センター, 教授 (30399555)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2019: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Keywords共生 / アブラムシ / ゲノム編集 / 抗菌ペプチド / 次世代シーケンシング / 遺伝子ネットワーク / 比較ゲノム
Outline of Final Research Achievements

Aphid species harbor an obligate endosymbiont, Buchnera aphidicola, within their specialized large cells called bacteriocytes and depend on Buchnera for essential nutrients. Aphid and Buchnera are well integrated physiologically and anatomically, which resulted in the absolute interdependence between the host and the symbiont. Using aphid-Buchnera symbiosis as a model, we aimed to understand the mechanisms and the evolution underlying the integrative genetic network in the symbiosis. We found that BCR peptides, which we have already reported their symbiotic organ-specific expression, have antimicrobial activity. We also argued the universality of the symbiotic antimicrobial peptide considering similar cases reported in other symbiotic systems. We succeeded in the development of CRISPR/Cas9 genome editing in the pea aphid, which would open a new avenue of aphid and symbiosis studies in the future.

Academic Significance and Societal Importance of the Research Achievements

近年「共生」は地球生態系や生命進化、そしてヒトの健康にも重要な役割を果たす事が認識されてきました。しかし宿主と共生者の間でどのような分子を使いどのような相互作用を通して共生が営まれているかについては、ほとんどわかっていません。今回、私たちは昆虫アブラムシから発見したBCRというペプチド(短いタンパク質)が抗菌活性を持つことを発見しました。この分子を使って共生細菌を制御していると考えられます。類似の例が他の動植物の共生においても報告されていることから、共生が進化する際の一般原理として注目されます。また、今回の研究の過程でアブラムシをゲノム編集により遺伝子操作する技術を開発することもできました。

Report

(4 results)
  • 2020 Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (24 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (4 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (18 results) (of which Int'l Joint Research: 4 results,  Invited: 6 results)

  • [Int'l Joint Research] Janelia Research Campus(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Janelia Research Campus(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] CNRS(フランス)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] HHMI Janelia Research Campus(米国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Antimicrobial Activities of Cysteine-rich Peptides Specific to Bacteriocytes of the Pea Aphid <i>Acyrthosiphon pisum</i>2019

    • Author(s)
      Uchi N, Fukudome M, Nozaki N, Suzuki M, Osuki K-I, Shigenobu S, Uchiumi T.
    • Journal Title

      Microbes and Environments

      Volume: 34 Issue: 2 Pages: 155-160

    • DOI

      10.1264/jsme2.ME18148

    • NAID

      130007668480

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Metabolic Integration of Bacterial Endosymbionts through Antimicrobial Peptides2017

    • Author(s)
      Mergaert Peter、Kikuchi Yoshitomo、Shigenobu Shuji、Nowack Eva C.M.
    • Journal Title

      Trends in Microbiology

      Volume: 25 Issue: 9 Pages: 703-712

    • DOI

      10.1016/j.tim.2017.04.007

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Genome editing in the pea aphid2020

    • Author(s)
      Shuji Shigenobu
    • Organizer
      19th Annual Workshop at Bellairs - Emerging Model Systems
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] NGSが切り拓く昆虫研究のフロンティア2019

    • Author(s)
      重信秀治
    • Organizer
      九州大学昆虫科学・新産業創生研究センター設立記念 キックオフシンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] NGSで切り拓く新しいモデル生物2019

    • Author(s)
      重信秀治
    • Organizer
      PacBioユーザーグループミーティング 2019
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Genomic Revelations of a Mutualism: Aphids and the Endosymbiont2019

    • Author(s)
      Shuji Shigenobu
    • Organizer
      The 2nd NIBB-Princeton Joint Symposium
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ゲノムから読み解く昆虫の不思議2019

    • Author(s)
      重信秀治
    • Organizer
      大学共同利用機関シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] CRISPR/Cas9 Genome Editing in the Pea Aphid2019

    • Author(s)
      重信秀治
    • Organizer
      日本進化学会第21回大会(札幌)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Ortholog Analyses Reveal Genomic Basis of Evolutionary Novelty - Lessons from Insect Genomes2019

    • Author(s)
      Shuji Shigenobu
    • Organizer
      The 67th NIBB Conference "Quest for Orthologs"
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] NGSが切り拓く昆虫研究のフロンティア2019

    • Author(s)
      重信秀治
    • Organizer
      日本畜産学会若手企画シンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] CRISPR/Cas9 Genome Editing in the Pea Aphid2019

    • Author(s)
      Shuji Shigenobu
    • Organizer
      Janelia Conference : New Genetic Tools for Non-Model Organisms
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ゲノム解読法の最前線2018

    • Author(s)
      重信秀治
    • Organizer
      日本植物学会第82回大会(広島)
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] オーソログ解析で解明する昆虫類の多様な新奇形質進化の遺伝子基盤2018

    • Author(s)
      重信秀治
    • Organizer
      日本進化学会第20回大会(東京)
    • Related Report
      2018 Annual Research Report
  • [Presentation] de novo of genome assembly of the pea aphid by a combination of long-read and short-read sequencing platforms2018

    • Author(s)
      Shuji Shigenobu
    • Organizer
      Nanopore London Calling 2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] アブラムシの性と生殖2017

    • Author(s)
      重信秀治
    • Organizer
      2017年度 性と生殖の懇談会
    • Related Report
      2017 Annual Research Report
  • [Presentation] アブラムシの生殖戦略--表現型可塑性と共生の視点から2017

    • Author(s)
      重信秀治
    • Organizer
      日本動物学会第88回大会(富山)
    • Related Report
      2017 Annual Research Report
  • [Presentation] 次世代シーケンシング時代の昆虫戦略2017

    • Author(s)
      重信秀治
    • Organizer
      日本動物学会第88回大会(富山)
    • Related Report
      2017 Annual Research Report
  • [Presentation] アブラムシのゲノミクス&ポストゲノミクス2017

    • Author(s)
      重信秀治
    • Organizer
      昆虫ポストゲノム研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Improvement of genome assembly of the pea aphid, Acyrthosiphon pisum, by a combination of PacBio and Nanopore technologies2017

    • Author(s)
      Shuji Shigenobu
    • Organizer
      Nanopore Community Meeting 2017 in New York
    • Related Report
      2017 Annual Research Report
  • [Presentation] アブラムシ細胞内共生における新規抗菌ペプチド様遺伝子の発現と機能2017

    • Author(s)
      重信秀治
    • Organizer
      日本進化学会第19回大会(京都)
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2022-05-23  

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