| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
The purpose of this study is to understand the genetic basis of the high environmental adaptability of Procambarus clarkii, which has been invaded to cold regions in a short period of time. Genome resequencing was conducted using genomic DNA of P. clarkii collected from Sapporo, Aomori, and Kamakura to estimate the loci involved in low temperature adaptation of the crayfish. As a result, we found several genes that respond to low temperature neighboring alleles that are highly frequent in a genome of the Sapporo population. Among these genes, the gene encoding Rho GTPase regulating protein showed a rapid increase in expression in the Sapporo population under low temperature conditions. These results suggest that a molecular pathway involving this gene may contribute to the adaptation of the Sapporo population to low temperatures.
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