| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Outline of Final Research Achievements |
In this study, we developed a technique to understand the behavior and physiological state of an organism using environmental nucleic acids (environmental DNA and RNA). The results showed that increased environmental DNA could be a marker of reproductive behavior, that environmental RNA was detectable, that a large number of cytoskeleton-related genes were detected in environmental water, and that mRNAs that could be candidate reproductive markers were present in the environment. This is the first study to demonstrate the applicability of environmental nucleic acids to the detection of animal physiological states and behaviors in the field. These results are particularly noteworthy because they expand the applicability of environmental nucleic acid analysis in the future.
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