Development of novel biological survey method using environmental DNA/RNA: estimating the behavior and states of underwater organisms
| Project/Area Number |
17H03735
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ecology/Environment
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| Research Institution | Kobe University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
山中 裕樹 龍谷大学, 理工学部, 講師 (60455227)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Keywords | 環境DNA / 環境RNA / 繁殖 / 生理状態 / RNA-seq / ゼブラフィッシュ / コイ / オオサンショウウオ / 繁殖行動 / RNA/DND比 / 分子生態 / 齢構成 |
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| Outline of Final Research Achievements |
In this study, we developed a technique to understand the behavior and physiological state of an organism using environmental nucleic acids (environmental DNA and RNA). The results showed that increased environmental DNA could be a marker of reproductive behavior, that environmental RNA was detectable, that a large number of cytoskeleton-related genes were detected in environmental water, and that mRNAs that could be candidate reproductive markers were present in the environment. This is the first study to demonstrate the applicability of environmental nucleic acids to the detection of animal physiological states and behaviors in the field. These results are particularly noteworthy because they expand the applicability of environmental nucleic acid analysis in the future.
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| Academic Significance and Societal Importance of the Research Achievements |
環境DNAなどの核酸を用いることで生物の状態を明らかにできる技術開発に成功したことは、希少種が再生産しているかなど生物多様性保全に関わる情報を非侵襲的に得ることなどを可能にする。また、環境中のmRNAなどは生物の生理状態を反映すると考えられ、水産養殖環境における感染症の早期発見など、応用的な利用も可能であると考えられる。このように本研究では基礎生物学的にも応用生物学的にも重要な手法の開発に成功した。
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Report
(4 results)
Research Products
(50 results)
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[Journal Article] Habitat selection and migration of the common shrimp, Palaemon paucidens in Lake Biwa, Japan - An eDNA - based study.2019
Author(s)
Wu, Q., Kawano, K., Ishikawa, T, Sakata, M. K., Nakao, R., Hiraiwa, M. K., Tsuji, S., Yamanaka, H. and Minamoto, T.
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Journal Title
Environmental DNA
Volume: -
Issue: 1
Pages: 1-10
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A simple method for preserving environmental DNA in water samples at ambient temperature by addition of cationic surfactant2017
Author(s)
Yamanaka, H., Minamoto, T., Matsuura, J., Sakurai, S., Tsui, S., Motozawa, H., Hongo, M., Sogo, Y., Kakimi, N., Teramura, I., Sugita, M., Baba, M., Kondo, A.
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Journal Title
Limnology
Volume: 17
Issue: 2
Pages: 233-241
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Environmental DNA enables detection of terrestrial mammals from forest pond water2017
Author(s)
Masayuki Ushio, Hisato Fukuda, Toshiki Inoue, Kobayashi Makoto, Osamu Kishida, Keiichi Sato, Koichi Murata, Masato Nikaido, Tetsuya Sado, Yukuto Sato, Masamichi Takeshita, Wataru Iwasaki, Hiroki Yamanaka, Michio Kondoh, and Masaki Miya
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Journal Title
Molecular Ecology Resources
Volume: 17(6):e
Issue: 6
Pages: 63-75
DOI
Related Report
Peer Reviewed
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