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Hierarchical understanding of the dynamics of epigenetic changes in characters and their control in plants

Research Project

Project/Area Number 17H03743
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Science in genetics and breeding
Research InstitutionHokkaido University

Principal Investigator

Kanazawa Akira  北海道大学, 農学研究院, 教授 (30281794)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2019: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2018: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Keywords遺伝子発現制御 / エピゲノム / 植物
Outline of Final Research Achievements

Focusing on epigenetic changes in gene expression, which are maintained through cell division without alterations in the DNA sequence, their dynamics in different layers including within gene, between alleles, and between cells and/or tissues were analyzed. In particular, transgene methylation that is induced during the growth of plants was characterized in terms of its relationship with changes in phenotypes, its stability across generations, and its changes via allelic interactions. In addition, changes in the insertional preference of a plant transposable element into chromosomal regions during evolution and the tissue specificity of its expression were explored.

Academic Significance and Societal Importance of the Research Achievements

エピジェネティックな機構による遺伝子発現の変化は、他の方法によってはなし得ない、DNAの塩基配列の変化を伴わない形質改変を可能にし、したがって、植物の形質を改変する新たな手法となり得る。一方、エピジェネティックな変化は必ずしも安定ではなく、可逆的であるという特徴を持つ。本研究の成果は、エピジェネティックな変化の誘導を新たな植物育種の方法として確立する上で考慮する必要のある、安定性を含む、この現象の特性に関する知見を提供した意義を持つ。

Report

(5 results)
  • 2022 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (10 results)

All 2022 2021 2019 2018 2017

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (8 results)

  • [Journal Article] Chromosomal distribution of soybean retrotransposon SORE-1 suggests its recent preferential insertion into euchromatic regions2018

    • Author(s)
      Nakashima, K., Abe, J. and Kanazawa, A.
    • Journal Title

      Chromosome Res.

      Volume: 26 Issue: 3 Pages: 199-210

    • DOI

      10.1007/s10577-018-9579-y

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcription of soybean retrotransposon SORE-1 is temporally upregulated in developing ovules2018

    • Author(s)
      Nakashima, K., Tsuchiya, M., Fukushima, S., Abe, J. and Kanazawa, A.
    • Journal Title

      Planta

      Volume: 248 Issue: 5 Pages: 1131-1137

    • DOI

      10.1007/s00425-018-3005-8

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] ペチュニアにおける葯の着色に顕在化する転写因子の機能的冗長性2022

    • Author(s)
      ゆはず 真白・原 涼子・金澤 章
    • Organizer
      日本遺伝学会第94回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ペチュニアにおいて葯の着色を誘導するアントシアニン生合成経路に関与する遺伝子と転写制御ネットワーク2022

    • Author(s)
      ゆはず 真白・原 涼子・金澤 章
    • Organizer
      日本育種学会第142回講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ペチュニアにおけるPHZの異所的発現はアントシアニン生合成後期段階の遺伝子の発現増強を介して葯の着色を誘導する2021

    • Author(s)
      原 涼子・金澤 章
    • Organizer
      日本育種学会第140回講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ダイズの新規な種皮着色突然変異体においてCHS遺伝子のinverted repeatが失われている2021

    • Author(s)
      ゆはず 真白・御厨 駿・森 あゆみ・千田 峰生・金澤 章
    • Organizer
      日本育種学会第140回講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ペチュニアにおけるアントシアニン生合成を制御するR2R3-MYB転写因子PHZの過剰発現による葯の着色2019

    • Author(s)
      原 涼子・木村芽生・金澤 章
    • Organizer
      日本育種学会第136回講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ペチュニアにおけるトランスジーンのエピアレル間の相互作用を介したコサプレッションによる表現型からの復帰2018

    • Author(s)
      木村芽生・長澤裕美・金澤 章
    • Organizer
      日本育種学会第134回講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ダイズのレトロトランスポゾンSORE-1の進化過程での挿入領域の変化2018

    • Author(s)
      中嶋健太・阿部 純・金澤 章
    • Organizer
      日本遺伝学会第90回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ダイズのレトロトランスポゾンSORE-1の日長依存的転写制御2017

    • Author(s)
      中嶋健太・阿部 純・金澤 章
    • Organizer
      日本育種学会第132回講演会
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2024-01-30  

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