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Analysis of dominant genic male-sterile rice for realizing a recurrent selection based on the genome shuffling

Research Project

Project/Area Number 17H03744
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Science in genetics and breeding
Research InstitutionTohoku University

Principal Investigator

Toriyama Kinya  東北大学, 農学研究科, 教授 (20183882)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2020: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Keywords育種学 / 遺伝学 / ゲノム / イネ / 遺伝子 / 植物
Outline of Final Research Achievements

Dominant male sterility is a powerful tool to apply outcross-based breeding system to autogamous crops such as rice. We previously obtained male sterile plants in an indica rice cultivar Lebed backcrossed with a japonica Taichung 65 (T65) and found the heterozygous Lebed allele of the male sterility gene, named LEBED-TAICHUNG 65 MALE STERILITY (LTMS), acts as dominant sporophytic pollen killer. We carried out map-based cloning of the LTMS gene and identified a candidate gene for LTMS. The LTMS gene was demonstrated to be the Lebed allele of the LTMS acting as dominant male sterility allale in a nuclear background of japonica rice, thus would be useful for recurrent selection breeding of japonica rice.

Academic Significance and Societal Importance of the Research Achievements

本研究をイネの品種改良に応用すると、自殖性作物のイネを効率的に他殖させ、ゲノムシャッフリングを行うことができる。トウモロコシなどの多殖性作物の育種法である循環選抜育種をジャポニカイネに応用できることになり、遺伝的多様性の大きい様々な優れたイネの品種改良を行うことが可能となる。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (25 results)

All 2021 2020 2019 2018 2017 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (20 results) (of which Int'l Joint Research: 5 results) Remarks (2 results)

  • [Journal Article] Fertility restoration of Chinese wild rice-type cytoplasmic male sterile rice by CRISPR/Cas9-mediated genome editing of nuclear-encoded RETROGRADE-REGULATED MALE STERILITY.2020

    • Author(s)
      Suketomo C, Kazama T, Toriyama K
    • Journal Title

      Plant Biotechnology

      Volume: 37 Issue: 3 Pages: 285-292

    • DOI

      10.5511/plantbiotechnology.20.0326b

    • NAID

      130007907470

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of cytoplasmic male sterile lines and restorer lines of various elite Indica Group rice cultivars using CW-CMS/Rf17 system2019

    • Author(s)
      Toriyama Kinya、Kazama Tomohiko、Sato Tadashi、Fukuta Yoshimichi、Oka Masaaki
    • Journal Title

      Rice

      Volume: 12 Issue: 1 Pages: 1-4

    • DOI

      10.1186/s12284-019-0332-8

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genetic analysis of male sterility obtained from a rice cultivar Lebed backcrossed with Taichung 652018

    • Author(s)
      Murakami Tetsuya、Kazama Tomohiko、Toriyama Kinya
    • Journal Title

      Rice

      Volume: 11 Issue: 1 Pages: 1-5

    • DOI

      10.1186/s12284-018-0222-5

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] mitoTALENを用いた遺伝子破壊によるイネTA型CMS原因遺伝子の同定2021

    • Author(s)
      髙塚 歩, 風間 智彦, 有村 慎一, 鳥山 欽哉
    • Organizer
      日本育種学会第139回講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] MitoTALEN-mediated mitochondrial gene-knockout revealed a cytoplasmic male sterility-causative gene in Oryza sativa cv. Tadukan.2021

    • Author(s)
      Takatsuka A, Kazama T, Arimura S, Toriyama K
    • Organizer
      KEYSTONE SYMPOSIA, Plant Genome Engineering: From Lab to Field
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] インディカイネ品種 Lebed に由来する雄性不稔遺伝子とその抑制遺伝子の解析2020

    • Author(s)
      藤田悠生,市田裕之,風間智彦,阿部知子,鳥山欽哉
    • Organizer
      日本育種学会第138回講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] インディカイネ品種Tadukanの細胞質に由来する雄性不稔性原因候補遺伝子の探索2020

    • Author(s)
      髙塚歩, 鳥山欽哉, 風間智彦
    • Organizer
      日本育種学会第138回講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] RT102型細胞質雄性不稔イネのミトコンドリアゲノム編集によって生じた新奇orfの発現解析2020

    • Author(s)
      大向詩穂, 鳥山欽哉, 有村慎一, 風間智彦
    • Organizer
      日本育種学会第138回講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] イネ Tadukan 型細胞質雄性不稔性のミトコンドリア原因遺伝子 orf312 の同定2020

    • Author(s)
      髙塚 歩, 風間 智彦, 有村 慎一, 鳥山 欽哉
    • Organizer
      第15回東北育種研究集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] orf352 を持つ RT102 型および WA 型細胞質雄性不稔性イネの分子遺伝学的比較2020

    • Author(s)
      大向 詩穂,鳥山 欽哉,有村 慎一,風間 智彦
    • Organizer
      第15回東北育種研究集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] イネ品種 Lebed に台中 65 号を戻し交雑して得られた雄性不稔系統における原因候補遺伝子の機能推定2020

    • Author(s)
      藤田 悠生, 市田 裕之, 風間 智彦, 阿部 知子, 鳥山 欽哉
    • Organizer
      第15回東北育種研究集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ミトコンドリア移行TALENを用いた細胞質雄性不稔性関連遺伝子orf352の破壊と表現型の調査2020

    • Author(s)
      大向詩穂, 鳥山欽哉, 有村慎一, 風間智彦
    • Organizer
      日本育種学会第137回講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] イネ品種 Lebed に台中 65 号を戻し交雑して得られた雄性不稔系統における原因候補遺伝子の解析2019

    • Author(s)
      藤田悠生, 風間智彦, 鳥山欽哉
    • Organizer
      日本育種学会第136回講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 核コードRETROGRADE-REGULATED MALE STERILITY 遺伝子のゲノム編集によるCW 型細胞質雄性不稔イネの稔性回復2019

    • Author(s)
      助友千尋, 風間智彦, 鳥山欽哉
    • Organizer
      日本育種学会第136回講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ミトコンドリア移行TALENを用いたCMS原因遺伝子候補 orf352 のノックアウト2019

    • Author(s)
      大向詩穂, 鳥山欽哉, 有村慎一, 風間智彦
    • Organizer
      日本育種学会第136回講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Knock-out of mitochondrial gene, orf352, using mitochondria-targeted TALEN2019

    • Author(s)
      Omukai S, Arimura S, Toriyama K, Kazama T
    • Organizer
      JSOL2019 (Japan Solanaceae Consortium 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ミトコンドリア移行TALENによるRT102型CMSイネミトコンドリアゲノムの構造変化と表現型の調査2019

    • Author(s)
      大向詩穂,鳥山欽哉,有村慎一,風間智彦
    • Organizer
      第14回東北育種研究集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Tadukan型細胞質雄性不稔性イネの稔性回復様式とミトコンドリア遺伝子の解析2019

    • Author(s)
      髙塚歩,風間智彦,鳥山欽哉
    • Organizer
      第14回東北育種研究集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Arms race between mitochondria and nuclei revealed by cytoplasmic male sterility/fertility restoration systems in rice2019

    • Author(s)
      Kinya Toriyama, Tomohiko Kazama
    • Organizer
      The 17Th International Symposium on Rice Functional Genomics
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fertility restoration of CW-type cytoplasmic male sterile rice by CRISPR/Cas9- mediated genome editing of nuclear-encoded RETROGRADE-REGULATED MALE STERILITY2019

    • Author(s)
      Chihiro Suketomo, Tomohiko Kazama, Kinya Toriyama
    • Organizer
      The 17Th International Symposium on Rice Functional Genomics
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] イネ品種LEBEDに台中65号を連続戻し交雑することで得られた雄性不稔系統における原因遺伝子のマッピング2018

    • Author(s)
      村上哲也・風間智彦・鳥山欽哉
    • Organizer
      日本育種学会第133回講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Dominant male sterility originated from an indica cultivar Lebed2018

    • Author(s)
      Murakami T, Kazama T, Toriyama K
    • Organizer
      International Hybrid Rice Symposium 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] イネ品種LEBEDに台中65号を連続戻し交雑することで得られた雄性不稔系統の遺伝学的解析2017

    • Author(s)
      村上哲也・風間智彦・鳥山欽哉
    • Organizer
      日本育種学会第132回講演会
    • Related Report
      2017 Annual Research Report
  • [Remarks] 環境適応生物工学研究室HP「お知らせ」

    • URL

      https://www.agri.tohoku.ac.jp/bioadp/PukiWiki/index.php?FrontPage

    • Related Report
      2019 Annual Research Report
  • [Remarks] 環境適応生物工学研究室HP「お知らせ」

    • URL

      http://www.agri.tohoku.ac.jp/bioadp/PukiWiki/index.php?2018

    • Related Report
      2018 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2022-12-28  

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