Genetic and biochemical studies of cytochrome P450 enzymes that regulate plant development and organ size.

• Project/Area Number: 17H03746
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: Kibi International University (2018-2019); The University of Tokyo (2017)
• Principal Investigator: Hibara Ken-ichiro 吉備国際大学, 農学部, 准教授 (10595713)
• Co-Investigator(Kenkyū-buntansha): 大西 利幸 静岡大学, 農学部, 准教授 (60542165)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000); Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
• Keywords: シトクロムP450 / CYP78遺伝子 / 植物発生 / 器官サイズ / 胚発生

Outline of Final Research Achievements

The following three results were obtained from this research project. (1) functional and expressional analyses of eight CYP78 genes in rice and genetical studies using Arabidopsis mutants have shown that CYP78 function is conserved, and that the substances they produce are produced locally in the plant body and are transported only to a limited extent. (2) Purification of CYP78 protein and measurement of enzymatic activity were difficult to perform by conventional methods. Purified CYP78 activities also differed from that of previous study. (3) From genetical studies and analyses of ge suppressor mutants, we identified three novel factors that are thought to act in the CYP78 regulatory pathway.

Academic Significance and Societal Importance of the Research Achievements

これまでに植物種を超えて、側生器官、種子、果実サイズを改変できることが報告されている遺伝子はCYP78遺伝子のみである。CYP78の生産する代謝産物を同定することにより、これまで育種によって改変されてきた果実、種子サイズや葉の大きさなどを物質投与により改変することができるかもしれない。本申請課題では、作物生産において有用な機能を持つCYP78遺伝子について機能、発現、遺伝的関係といった様々な基礎的情報を取得することができた。本課題から得られた成果をさらに発展させることにより、CYP78の代謝産物同定や分子メカニズムの解明につながることを期待している。

Research Products

• [Journal Article] Rhizotaxis Modulation in Arabidopsis Is Induced by Diffusible Compounds Produced during the Cocultivation of Arabidopsis and the Endophytic Fungus Serendipita indica (2020)
  • Author(s): Inaji Aoi、Okazawa Atsushi、Taguchi Taiki、Nakamoto Masatoshi、Katsuyama Nao、Yoshikawa Ryoka、Ohnishi Toshiyuki、Waller Frank、Ohta Daisaku
  • Journal Title: Plant and Cell Physiology Volume: 61 Issue: 4 Pages: 838-850
  • DOI: 10.1093/pcp/pcaa008
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Specification of basal region identity after asymmetric zygotic division requires mitogen-activated protein kinase 6 in rice (2019)
  • Author(s): Ishimoto K., Sohonahra S., Kishi-Kaboshi M., Itoh J-i., Hibara K-i., Sato Y., Watanabe T., Abe K., Miyao A., Nosaka-Takahashi M., Suzuki T., Ta N. K., Shimizu-Sato S., Suzuki T., Toyoda A., Takahashi H., Nakazono M., Nagato Y., Hirochika H., Sato Y.
  • Journal Title: Development Volume: 146
  • DOI: 10.1242/dev.176305
  • Peer Reviewed / Open Access
• [Journal Article] Recent advances in brassinosteroid biosynthetic pathway: insight into novel brassinosteroid shortcut pathway (2018)
  • Author(s): Ohnishi Toshiyuki
  • Journal Title: Journal of Pesticide Science Volume: 43 Issue: 3 Pages: 159-167
  • DOI: 10.1584/jpestics.D18-040
  • NAID: 130007437206
  • ISSN: 1348-589X, 1349-0923
  • Year and Date: 2018-08-20
  • Peer Reviewed / Open Access
• [Journal Article] 「香り」や香気配糖体が織りなす化学防御システム-「香り」や香気配糖体が植物の身を守る- (2018)
  • Author(s): 大西利幸
  • Journal Title: 日本農薬学会誌 Volume: 43 Pages: 65-66
  • NAID: 130007691654
  • Peer Reviewed
• [Journal Article] Overexpression of geraniol synthase induces heat stress susceptibility in Nicotiana tabacum (2018)
  • Author(s): Hamachi Ashita、Nisihara Masahiro、Saito Shiori、Rim Hojun、Takahashi Hideyuki、Islam Monirul、Uemura Takuya、Ohnishi Toshiyuki、Ozawa Rika、Maffei Massimo E.、Arimura Gen-ichiro
  • Journal Title: Planta Volume: 249 Issue: 1 Pages: 235-249
  • DOI: 10.1007/s00425-018-3054-z
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Identification of a Hexenal Reductase That Modulates the Composition of Green Leaf Volatiles (2018)
  • Author(s): Tanaka Toshiyuki、Ikeda Ayana、Shiojiri Kaori、Ozawa Rika、Shiki Kazumi、Nagai-Kunihiro Naoko、Fujita Kenya、Sugimoto Koichi、Yamato Katsuyuki T.、Dohra Hideo、Ohnishi Toshiyuki、Koeduka Takao、Matsui Kenji
  • Journal Title: Plant Physiology Volume: 178 Issue: 2 Pages: 552-564
  • DOI: 10.1104/pp.18.00632
  • Peer Reviewed / Open Access
• [Journal Article] Silkworms suppress the release of green leaf volatiles by mulberry leaves with an enzyme from their spinnerets (2018)
  • Author(s): Takai Hiroki、Ozawa Rika、Takabayashi Junji、Fujii Saki、Arai Kiriko、Ichiki Ryoko T.、Koeduka Takao、Dohra Hideo、Ohnishi Toshiyuki、Taketazu Sakura、Kobayashi Jun、Kainoh Yooichi、Nakamura Satoshi、Fujii Takeshi、Ishikawa Yukio、Kiuchi Takashi、Katsuma Susumu、Uefune Masayoshi、Shimada Toru、Matsui Kenji
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 11942-11942
  • DOI: 10.1038/s41598-018-30328-6
  • NAID: 120006510284
  • Peer Reviewed / Open Access
• [Journal Article] 1-Octen-3-ol Is Formed from Its Glycoside during Processing of Soybean [Glycine max (L.) Merr.] Seeds (2018)
  • Author(s): Matsui Kenji、Takemoto Hiroyuki、Koeduka Takao、Ohnishi Toshiyuki
  • Journal Title: Journal of Agricultural and Food Chemistry Volume: 66 Issue: 28 Pages: 7409-7416
  • DOI: 10.1021/acs.jafc.8b01950
  • Peer Reviewed / Open Access
• [Journal Article] Structure-Based Chemical Design of Abscisic Acid Antagonists That Block PYL?PP2C Receptor Interactions (2018)
  • Author(s): Takeuchi Jun、Mimura Naoki、Okamoto Masanori、Yajima Shunsuke、Sue Masayuki、Akiyama Tomonori、Monda Keina、Iba Koh、Ohnishi Toshiyuki、Todoroki Yasushi
  • Journal Title: ACS Chemical Biology Volume: 13 Issue: 5 Pages: 1313-1321
  • DOI: 10.1021/acschembio.8b00105
  • Peer Reviewed
• [Journal Article] The effects of gene disruption of Kre6-like proteins on the phenotype of β-glucan-producing Aureobasidium pullulans. (2018)
  • Author(s): Uchiyama H, Iwai A, Dohra H, Ohnishi T, Kato T, Park EY
  • Journal Title: Appl Microbiol Biotechnol Volume: 102 Issue: 10 Pages: 4467-4475
  • DOI: 10.1007/s00253-018-8947-z
  • NAID: 120006543632
  • Peer Reviewed
• [Journal Article] Transcriptional switch for programmed cell death in pith parenchyma of sorghum stems. (2018)
  • Author(s): Fujimoto M, Sazuka T, Oda Y, Kawahigashi H, Wu J, Takanashi H, Ohnishi T, Yoneda JI, Ishimori M, Kajiya-Kanegae H, Hibara KI, Ishizuna F, Ebine K, Ueda T, Tokunaga T, Iwata H, Matsumoto T, Kasuga S, Yonemaru JI, Tsutsumi N
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 115 Issue: 37
  • DOI: 10.1073/pnas.1807501115
  • Peer Reviewed / Open Access
• [Journal Article] Conserved functional control, but distinct regulation of cell proliferation in rice and Arabidopsis leaves revealed by comparative analysis of GRF-INTERACTING FACTOR 1 orthologs. (2018)
  • Author(s): Shimano, S., Hibara, K., Furuya, T., Arimura, S., Tsukaya,H. and Itoh, J.I.
  • Journal Title: Development Volume: 175 Issue: 7 Pages: 159624-159624
  • DOI: 10.1242/dev.159624
  • Peer Reviewed / Open Access
• [Journal Article] LEAF LATERAL SYMMETRY1, a member of the WUSCHEL-RELATED HOMEOBOX3 gene family, regulates lateral organ development differentially from other paralogs, NARROW LEAF2 and NARROW LEAF3 in rice. (2017)
  • Author(s): Eriko, H., Yew, C.L., Yoshikawa, T., Sato, Y., Hibara, K. and Itoh, J.I.
  • Journal Title: Plant and Cell Physiology Volume: 59 Issue: 2 Pages: 376-391
  • DOI: 10.1093/pcp/pcx196
  • Peer Reviewed / Open Access
• [Journal Article] “Hidden” terpenoids in plants: their biosynthesis, localisation and ecological roles (2017)
  • Author(s): Yazaki K., Arimura G., Ohnishi T.
  • Journal Title: Plant and Cell Physiology Volume: 58 Issue: 10 Pages: 1615-1621
  • DOI: 10.1093/pcp/pcx123
  • Peer Reviewed / Open Access
• [Journal Article] Occurrence of brassinosteroids in non-flowering land plants, liverwort, moss, lycophyte and fern. (2017)
  • Author(s): Yokota T, Ohnishi T, Shibata K, Asahina M, Nomura T, Fujita T, Ishizaki K, Kohchi T.
  • Journal Title: Phytochemistry Volume: 136 Pages: 46-55
  • DOI: 10.1016/j.phytochem.2016.12.020
  • Peer Reviewed
• [Presentation] オオムギMANY-NODED DWARF遺伝子群による葉間期制御 (2020)
  • Author(s): 桧原 健一郎, 武田 真, 伊藤 純一
  • Organizer: 日本育種学会第137回講演会
• [Presentation] イネの生育相転換に関与するqJA1、qJA2が地域適応性に及ぼす効果 (2020)
  • Author(s): 藤本 源、渡部 太緒、荒川 恵理、小出 陽平、門田 有希、寺石 政義、伊藤 純一、桧原 健一郎、長戸 康郎、奥本 裕、吉川 貴徳
  • Organizer: 日本育種学会第137回講演会
• [Presentation] イネ種子のγ-オリザノール含量を規定する候補因子の探索 (2020)
  • Author(s): 船越 孝之, 桧原 健一郎, 小川 拓水, 手塚 孝弘, 太田 大策, 横井 修司
  • Organizer: 日本育種学会第137回講演会
• [Presentation] 芋焼酎の香気に関与するサツマイモ由来香気配糖化酵素の解明 (2019)
  • Author(s): 塚原壮彦, 海山香菜子, 肥塚崇男, 竹本裕之, 竹内純, 大西利幸
  • Organizer: 第63回香料・テルペンおよび精油化学に関する討論会
• [Presentation] バラの香気成分の貯蔵メカニズム (2019)
  • Author(s): 中馬かれん, 肥塚崇男, 小木曽こはる, 勝又章椰, 鈴木秀幸, 竹内純, 轟泰司, 佐藤浩平,間瀬暢之, 竹本裕之, 渡辺修治, 大西利幸
  • Organizer: 第63回香料・テルペンおよび精油化学に関する討論会
• [Presentation] The biosynthetic machinery of rose aroma mono-glucosides. (2019)
  • Author(s): Karen Chuman, Koharu Ogiso, Fumiya Katsumat, Takao Koeduka Yukie Ohba, Hideyuki Suzuki, Jun Takeuchi, Yasushi Todoroki, Kouhei Sato, Mase. Watanabe Naoharu and Toshiyuki Ohnishi
  • Organizer: TERPNET 2019 - The 14th International Meeting on Biosynthesis, Function and Synthetic Biology of Isoprenoid
  • Int'l Joint Research
• [Presentation] 胚サイズを制御するシトクロムP450酵素の機能解明に向けた取り組み (2019)
  • Author(s): 桧原 健一郎、水野 泉
  • Organizer: 種子生理生化学研究会第40回年会
• [Presentation] イネGE遺伝子のシュート再生過程における機能解析 (2019)
  • Author(s): 八田 大成、水野 泉、桧原 健一郎、伊藤 純一
  • Organizer: 日本育種学会第135回講演会
• [Presentation] 植物発生・器官サイズを制御するシトクロムP450の代謝産物、シグナル伝達経路の解明に向けて (2018)
  • Author(s): 桧原 健一郎
  • Organizer: ナノ・マイクロ横断型人材育成セミナー
  • Invited
• [Presentation] 植物の成長制御に関与する CYP78のイネのカルス組織に対する効果 (2018)
  • Author(s): 八田 大成、水野 泉、桧原 健一郎、伊藤 純一
  • Organizer: 日本育種学会第134回講演会
• [Presentation] 胚－胚乳サイズ制御と新しい植物ホルモン (2018)
  • Author(s): 桧原健一郎
  • Organizer: 岡山大植物研・作物イノベーション研究ワークショップ（第1回）
  • Invited
• [Presentation] 植物の生長や化学防御に寄与する二次代謝産物の生合成研究 (2017)
  • Author(s): 大西利幸
  • Organizer: 第35回日本植物細胞分子生物学会
  • Invited
• [Presentation] Volatile C13-norisoprenoid and monoterpene alcohols contribute sweet muscatel-like scent in Oolong tea “Oriental Beauty” manufactured by Camellia sinensis in response to attacks by insect herbivores (2017)
  • Author(s): Koujirou Totsuka, Naoya Sakai, Tsuyoshi Katsuno, Naoharu Watanabe, Toshiyuki Ohnishi
  • Organizer: The 13th International Meeting on Biosynthesis, Function and Synthetic Biology
  • Int'l Joint Research