| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2020: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
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| Outline of Final Research Achievements |
It is well know that mitochondria and nucleus are interdependent. DNA demethylases in the nucleus require Fe-S clusters as cofactors. The biogenesis of this Fe-S cofactor starts from glutathione, which is exported through mitchondorial ATM3 transporter. We previously identified several Arabidopsis mutants related to this cytosolic Fe-S cluster biogenesis pathway, based on the phenotype that do not activate the imprinted FWA expression. In this study, we focus on the OsATM3 gene and generate its knock out plants by CRISPR/Cas9 system to understand role of ATM3. As a result, we found that phenotype of osatm2-/- is closely resembles to DNA demethylase mutant in the endosperm.
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