Study of endogenous factors of nicotine detected in tea
| Project/Area Number |
17H03756
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Crop production science
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| Research Institution | Shizuoka University |
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Principal Investigator |
Morita Akio 静岡大学, 農学部, 教授 (20324337)
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| Co-Investigator(Kenkyū-buntansha) |
荻野 暁子 国立研究開発法人農業・食品産業技術総合研究機構, 果樹茶業研究部門, 主任研究員 (70370567)
古川 一実 沼津工業高等専門学校, 物質工学科, 准教授 (90353151)
一家 崇志 静岡大学, 農学部, 准教授 (90580647)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2019: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Keywords | 茶 / ニコチン / 遺伝子組換え / 工芸作物 / チャ / ジャスモン酸 |
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| Outline of Final Research Achievements |
Tea nicotine biosynthesis may be regulated by a few genes. In addition, the activation of the polyamine synthesis pathway was associated with nicotine content, suggesting that there is another biosynthetic pathway for nicotine biosynthesis in tea plant that is not mediated by CsPMT.
It is important to control the catechin content in plants and to select strains that are resistant to the antimicrobial action of catechins, as well as to control the catechin content in plants. Furthermore, it was suggested that the transformation by bombardment method was more suitable for the transformation of tea plant.
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| Academic Significance and Societal Importance of the Research Achievements |
茶で微量に検出されたニコチンについては，内因性由来であることをある程度証明できた．それらを直接的に証明するためには，まだ数ステップの研究が必要であるが，その実験系に必要な情報基盤について整備できた．特に，チャの遺伝子組換え植物体作出については，本研究だけでなく，茶業関連の機能性解析や将来の育種など，様々な研究への応用が期待できる．
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Quantitative validation of nicotine production in tea (Camellia sinensis L.).2018
Author(s)
Ikka T, Yamashita H, Kurita I, Tanaka Y, Taniguchi F, Ogino A, Takeda K, Horie N, Hojo H, Nanjo F, Morita A.
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Journal Title
PLoS ONE
Volume: 13(4)
Issue: 4
Pages: 0195422-0195422
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Presentation] High-sensitivity determination and quantification of minor amounts of nicotine in tea with LC-MS/MS.2017
Author(s)
Hiroto Yamashita, Ikuya Kurita, Takashi Ikka, Fumiya Taniguchi, Akiko Ogino, Kazuyuki Takeda, Nobuyuki Horie, Hiroshi Hojo, Fumio Nanjo, Akio Morita
Organizer
Plant Biology 2017 (American Society of Plant Biologists, Honolulu, Hawaii, USA)
Related Report
Int'l Joint Research
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