Role of endoplasmic reticulum-plasma membrane system in regulation of Oryza sativa endosperm development

• Project/Area Number: 17H03759
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Crop production science
• Research Institution: Ehime University
• Principal Investigator: Onda Yayoi 愛媛大学, 農学研究科, 准教授 (70368463)
• Co-Investigator(Kenkyū-buntansha): 野並 浩 愛媛大学, 農学研究科, 教授 (00211467)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2017: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
• Keywords: 植物 / 酵素 / ジスルフィド結合 / 酵素蛋白質 / ジスルフィド / 蛋白質

Outline of Final Research Achievements

In this study, we show the key role of thiol-disulfide oxidoreductase, PDI, in actin cytoskeleton structure. When exposed to oxidative stress, Oryza sativa cells accumulated intermolecularly disulfide-bonded high-molecular-weight structures of actin. During recovery from oxidative stress, PDI cleaved disulfide bonds in high-molecular-weight structures of actin. Based on these results, we propose a PDI-dependent reductive pathway that promotes degradation of nonnative-disulfide-bonded structures of actin and reorganization of the actin cytoskeleton.

Academic Significance and Societal Importance of the Research Achievements

環境変動下，イネ細胞の生長制御はコメの品質・収量を決定する重要な課題である。本研究はPDI酸化系が小胞体において貯蔵蛋白質のジスルフィド架橋形成を触媒し酸化的折り畳みを促進するだけでなく，PDI還元系がサイトゾルにおいてアクチン構造体のジスルフィド架橋開裂を触媒しその分解を促進することを示した。本研究成果はオルガネラ運搬や細胞壁構築の駆動基盤である細胞骨格ダイナミクスの新規制御機構を示唆する。

Research Products

• [Journal Article] Thiol-disulfide oxidoreductase PDI1;1 regulates actin structures in Oryza sativa root cells (2022)
  • Author(s): Onda, Y., Okino, T.
  • Journal Title: FEBS Letters Volume: 596 Issue: 23 Pages: 3015-3023
  • DOI: 10.1002/1873-3468.14445
  • Peer Reviewed / Open Access
• [Journal Article] On-site single pollen metabolomics reveals varietal differences in phosphatidylinositol synthesis under heat stress conditions in rice (2020)
  • Author(s): Wada H, Hatakeyama Y, Nakashima T, Nonami H, Erra-Balsells R, Hakata M, Nakata K, Hiraoka K, Onda Y, Nakano H
  • Journal Title: Scientific Reports Volume: 10(1) Issue: 1 Pages: 2013-2013
  • DOI: 10.1038/s41598-020-58869-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Multiple strategies for heat adaptation to prevent chalkiness in the rice endosperm (2019)
  • Author(s): Wada H, Hatakeyama Y, Onda Y, Nonami H, Nakashima T, Erra-Balsells R, Morita S, Hiraoka K, Tanaka F, Nakano H
  • Journal Title: Journal of Experimental Botany Volume: 70(4) Issue: 4 Pages: 1299-1311
  • DOI: 10.1093/jxb/ery427
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Evidence for preservation of vacuolar compartments during foehn-induced chalky ring formation of Oryza sativa L. (2018)
  • Author(s): Hatakeyama Y, Masumoto-Kubo C, Nonami H, Morita S, Hiraoka K, Onda Y, Nakashima T, Nakano N, and Wada H
  • Journal Title: Planta Volume: 248(5) Issue: 5 Pages: 1263-1275
  • DOI: 10.1007/s00425-018-2975-x
  • Peer Reviewed / Open Access
• [Presentation] 水ストレスに対するミトコンドリア代謝応答の経時的評価 (2018)
  • Author(s): 中田佳祐、和田博史、恩田弥生、Rosa Erra-Basells、野並浩
  • Organizer: Joint Conference on Environmental Engineering in Agriculture 2018
• [Presentation] In situ picolitre pressure-probe-electrospray-ionization mass spectrometry for monitoring single cell metabolites change (2018)
  • Author(s): Nakata, K., Wada, H., Onda, Y., Erra-Balsells, R., Nonami, H.
  • Organizer: Mass Spectrometry and Proteomics 2018
• [Presentation] 高温登熟環境下での背白粒発生及び窒素施用による品質改善機構の品種間差異 (2018)
  • Author(s): 畠山友翔、中野洋、恩田弥生、野並浩、Rosa Erra-Balsells、中島大賢、平岡賢三、和田博史
  • Organizer: 第245回日本作物学会講演会
• [Presentation] Disulfide bond formation: redox-based regulation of organelle development in plant cells (2018)
  • Author(s): Onda, Y.
  • Organizer: 東京工業大学化学生命科学研究所国際フォーラム2018
  • Int'l Joint Research / Invited
• [Presentation] The role of cytoplasmic streaming in osmotic adjustment in tomato trichome cells under water stress (2017)
  • Author(s): Nakata, K., Wada, H., Onda, Y., Erra-Balsells, R., Nonami, H.
  • Organizer: 2017 CIGR World Workshop
  • Int'l Joint Research
• [Presentation] イネ胚乳細胞における小胞体環境の制御機構 (2017)
  • Author(s): 恩田弥生、紙田昂輝、野並浩
  • Organizer: 日本生物環境工学会2017年大会
• [Presentation] Development of the on-site live cell metabolomics performable in controlled environment (2017)
  • Author(s): Wada, H., Nonami, H., Tanaka, K., Nakashima, T., Hatakeyama, Y., Nakano, H., Onda, Y., Hiraoka, K., Hakata, M., Morita, S.
  • Organizer: SEB (Society for Experimental Biology) Gothenburg 2017
  • Int'l Joint Research
• [Presentation] Nitrogen application reverses heat-induced rice chalkiness: evidence for organelle rearrangement due to the recovery of protein synthesis in endosperm cells (2017)
  • Author(s): Hatakeyama, Y., Onda, Y., Nonami, H., Nakashima, T., Nakano, H., Erra-Balsells, R., Hiraoka, K., Wada, H.
  • Organizer: SEB (Society for Experimental Biology) Gothenburg 2017
  • Int'l Joint Research