| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2020: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2019: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2018: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Outline of Final Research Achievements |
Seven CYC2 genes, which had been reported to be involved in ray floret formation in Senecio vulgaris, were isolated from a diploid wild species of Chrysanthemum, Chrysanthemum seticuspe. Spatiotemporal expression analyses of three CYC2 genes with high expression levels in the early stage of capitulum development showed that CYC2 genes were expressed in stamens and pistils of disc florets, as well as petals of ray florets. Gene knock-out by CRISPR/Cas9 genome editing system targeting CYC2 gene resulted in multiple transformants lacking several bases within the target site of CYC2 gene, and phenotypic analysis of these transformants showed a tendency for ray florets to be converted to disc florets. The results of gene disruption by genome editing also suggested that the CYC2 gene is involved in florets formation not only in ray florets but also in disc florets.
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