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New approaches to visualizing freezing behaviors in plant tissues and their mechanisms involved

Research Project

Project/Area Number 17H03763
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticultural science
Research InstitutionThe University of Tokyo

Principal Investigator

Ishikawa Masaya  東京大学, 大学院農学生命科学研究科(農学部), 特任研究員 (90355727)

Co-Investigator(Kenkyū-buntansha) 寺田 康彦  筑波大学, 数理物質系, 准教授 (20400640)
福田 健二  東京大学, 大学院農学生命科学研究科(農学部), 教授 (30208954)
朽津 和幸  東京理科大学, 理工学部応用生物科学科, 教授 (50211884)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2020: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Keywords水 / 凍結 / 耐寒性 / 凍結様式 / MRI / 氷核活性 / 可視化 / 越冬 / イメージング / 凍結制御 / 凍結傷害 / 凍結過程 / 耐寒性・凍結傷害 / 環境ストレス / 植物 / 非破壊可視化
Outline of Final Research Achievements

The major component of plant tissues is water. Water freezes at sub-zero temperatures. Wintering woody perennials are exposed to recurrent freezing temperatures. How do these plant organs can avoid lethal intracellular freezing? In this study, noninvasive visualization of freezing behaviors in these thick complicated plant organs was successfully achieved using high resolution temperature-variable MRI. The results show that the method is very powerful in investigating the diversity, dynamics and mechanisms of freezing behaviors. We also successfully constructed a temperature-variable probe for MRI system in Japan and successfully visualized freezing behaviors of plant tissues.

Academic Significance and Societal Importance of the Research Achievements

豪州Western Sydney大学と共同で、高分解能温度可変MRIを利用した植物の凍結様式の多様性や動態、メカニズムの可視化解析の開発に成功した。また、国内にも、稼働できる温度可変MRIが完成し、大型試料も含め、植物の凍結様式や凍結過程の解析に利用できることになった。これにより世界的に皆無であったMRIのマシンタイムが大幅に増加し、研究の進展が期待される。今後、撮像方法を工夫して、様々な解析に利用できるようにする計画で、耐寒性機構や凍霜害回避法の開発に寄与することが可能である。

Report

(5 results)
  • 2022 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (33 results)

All 2022 2021 2020 2019 2018 2017 Other

All Int'l Joint Research (4 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results) Presentation (25 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results) Book (2 results)

  • [Int'l Joint Research] Western Sydney大学/Nanoscale Organisation and Dynamics/National Imaging Facility(オーストラリア)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Western Sydney大学/Nanoscale Organisation and Dynamics/National Imaging Facility(オーストラリア)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Western Sydney大学/Nanoscale Organisation and Dynamics/National Imaging Facility(オーストラリア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Western Sydney大学/Nanoscale Organisation and Dynamics/National Imaging Facility(オーストラリア)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Preferential freezing avoidance localized in anthers and embryo sacs in wintering Daphne kamtschatica var. jezoensis flower buds visualized by MRI2022

    • Author(s)
      Ishikawa M, Ide H, Tsujii T, Stait-Gardner T, Kubo H, Matsushita N, Fukuda K, Price W, Arata Y
    • Journal Title

      Plant Cell and Environment

      Volume: in press Issue: 7 Pages: 2109-2125

    • DOI

      10.1111/pce.14255

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] ササ類に含まれる氷核活性物質の探索2020

    • Author(s)
      久保 光,石川雅也,松下範久,福田健二
    • Journal Title

      低温生物工学会誌

      Volume: 66 Pages: 105-109

    • NAID

      130007991372

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] 耐寒性植物器官の凍結様式の多様性解析手段としての温度可変MRI2022

    • Author(s)
      石川雅也,T. Stait-Gardner,久保光,松下範久,福田健二,W.S. Price
    • Organizer
      第63回日本植物生理学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 温度可変MRマイクロイメージングシステムを用いた高山植物の凍結過程の評価2022

    • Author(s)
      籾山直人,高川直也,石川雅也,寺田康彦
    • Organizer
      第67回低温生物工学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 植物器官の凍結様式の多様性・詳細を高分解能MRIで探索する2022

    • Author(s)
      石川雅也,T. Stait-Gardner,久保光,松下範久,福田健二,W.S. Price
    • Organizer
      第67回低温生物工学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 温度可変MRマイクロイメージングシステムを用いた高山植物の凍結過程の解析2022

    • Author(s)
      籾山直人,石川雅也,寺田康彦
    • Organizer
      第26回NMRマイクロイメージング研究会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 石川雅也2022

    • Author(s)
      MRI による凍結様式可視化解析と凍結制御要因
    • Organizer
      日本木材学会組織と材質研究会2022年冬季シンポジウム「樹木の越冬メカニズム」
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 高分解能MRIを用いた複雑な植物器官の凍結様式の非破壊3Dイメージング2021

    • Author(s)
      石川雅也,T. Stait-Gardner,久保光,福田健二,W.S. Price
    • Organizer
      62回日本植物生理学会 松江
    • Related Report
      2019 Annual Research Report
  • [Presentation] 高分解能MRIを用いた凍結バリア部位の3D可視化解析2021

    • Author(s)
      石川雅也,T. Stait-Gardner,久保光,福田健二,W.S. Price
    • Organizer
      第66回低温生物工学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 花芽凍結観察用温度可変MRマイクロイメージングシステムの開発2021

    • Author(s)
      宮坂知樹 ,髙川直也, 石川雅也, 寺田康彦
    • Organizer
      第66回低温生物工学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 亜熱帯性タケ類と温帯性ササ類における氷核活性物質の特性2021

    • Author(s)
      久保光, 石川雅也, 松下範久, 福田健二
    • Organizer
      第66回低温生物工学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 温度可変 MRI による植物および食品サンプルの低温撮像2021

    • Author(s)
      宮坂知樹, 髙川直也, 石井寛崇, 石川雅也, 寺田康彦
    • Organizer
      第25回NMRマイクロイメージング研究会P5-8
    • Related Report
      2019 Annual Research Report
  • [Presentation] Visualization of freezing behaviors in plant organs: diversity and mechanisms involved.2021

    • Author(s)
      Ishikawa M.
    • Organizer
      12th International Plant Cold Hardiness Seminar
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 耐寒性植物の凍結様式詳細の3D非破壊可視化解析2020

    • Author(s)
      石川 雅也, Stait-Gardner T., 久保 光, 松下 範久, 福田 健二, Price W.S.
    • Organizer
      第84回日本植物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Development of variable-temperature MR microimaging system with a large-diameter solenoid coil for a vertical wide bore superconducting magnet.2020

    • Author(s)
      Takagawa N, Ishikawa M, Terada Y.
    • Organizer
      ISMRM 28th Annual Meeting, Sydney
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] チシマザサにおける氷核活性物質の探索2020

    • Author(s)
      久保光・石川雅也・松下範久・福田健二
    • Organizer
      第131回日本森林学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ササ類の氷核活性物質の探索2020

    • Author(s)
      久保光、石川雅也、松下範久、福田健二
    • Organizer
      第65回低温生物工学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 高分解能MRIを用いた植物の凍結様式の非破壊3D可視化解析2020

    • Author(s)
      石川雅也,T. Stait-Gardner,久保光,松下範久,福田健二,朽津和幸,W.S. Price
    • Organizer
      第65回低温生物工学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] MRI によるレンギョウ枝の凍結過程のセミリアルタイム可視化解析2019

    • Author(s)
      石川雅也,Timothy Stait-Gardner,村川裕基,山崎秀幸,朽津和幸,William S. Price
    • Organizer
      第60回日本植物生理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 高分解能MRIを用いた植物の凍結過程非破壊可視化法の開発2019

    • Author(s)
      石川雅也,T. Stait-Gardner,村川裕基,山崎秀幸,朽津和幸,福田健二, W.S. Price
    • Organizer
      第64回低温生物工学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] MRIを用いた植物の凍結過程非破壊可視化法の開発2019

    • Author(s)
      石川雅也,T. Stait-Gardner,村川裕基,山崎秀幸,朽津和幸,福田健二,W.S. Price
    • Organizer
      第83回植物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] MRI によるレンギョウ枝の凍結過程のセミリアルタイム可視化解析2019

    • Author(s)
      石川雅也,Timothy Stait-Gardner,村川裕基,山崎秀幸,朽津和幸,William S. Price
    • Organizer
      日本植物生理学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Studies on visualization of freezing behaviors and ice nucleation activity of plant tissues.2018

    • Author(s)
      Ishikawa M.
    • Organizer
      11th International Plant Cold Hardiness Seminar, Wisconsin
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 最新MRIを用いた耐寒性木本組織の凍結挙動の可視化解析2018

    • Author(s)
      石川雅也,T. Stait-Gardner,村川裕基,山崎秀幸,朽津和幸,W.S. Price
    • Organizer
      低温生物工学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] レンギョウ枝髄における凍結開始過程の詳細の可視化解析2017

    • Author(s)
      石川雅也,村川裕基,山崎秀幸,朽津和幸,T. Stait-Gardner,W.S. Price
    • Organizer
      低温生物工学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Visualization of freezing behaviors in cold hardy plant tissues using MRI and infra-red thermography.2017

    • Author(s)
      Ishikawa M, Yamazaki H, Murakawa H, Kuchitsu K, Price WS
    • Organizer
      International Symposium on Imaging Frontier 2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] Visualization of freeze initiation in the pith of Forsythia stems using infra-red thermography2017

    • Author(s)
      Ishikawa M, Murakawa H, Yamazaki H, Kuchitsu K
    • Organizer
      第58回日本植物生理学会
    • Related Report
      2017 Annual Research Report
  • [Book] Ice Nucleation Activity in Plants: The Distribution, Characterization, and Their Roles in Cold Hardiness Mechanisms. In: Iwaya-Inoue M., Sakurai M., Uemura M. (eds) Survival Strategies in Extreme Cold and Desiccation. Advances in Experimental Medicine and Biology, vol 1081.2018

    • Author(s)
      Masaya Ishikawa, Hideyuki Yamazaki,Tadashi Kishimoto, Hiroki Murakawa, Timothy Stait-Gardner, Kazuyuki Kuchitsu, William S. Price
    • Publisher
      Springer, Singapore
    • ISBN
      9789811312434
    • Related Report
      2017 Annual Research Report
  • [Book] 植物由来の凍結制御物質の探索と機能評価 in バイオテクノロジーシリーズ 不凍タンパク質の機能と応用(津田栄編)2018

    • Author(s)
      石川雅也
    • Total Pages
      290
    • Publisher
      シーエムシー出版
    • ISBN
      9784781313399
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2024-01-30  

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