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Molecular basis of plant immune respnses against plant viruses

Research Project

Project/Area Number 17H03770
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant protection science
Research InstitutionThe University of Tokyo

Principal Investigator

YAMAJI Yasuyuki  東京大学, 大学院農学生命科学研究科(農学部), 教授 (40345187)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2020: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Keywordsレクチン抵抗性 / 劣性抵抗性 / 植物ウイルス / 植物免疫 / RNAサイレンシング
Outline of Final Research Achievements

Plant immunity is an important area of research for increasing food production, and although the initial process of pathogen recognition by plants has been intensively studied, how plant immune responses ultimately act on pathogens to inhibit infection has remained largely unexplored. In this study, we focused on lectin-mediated resistance, which was found by our group to be a major plant immune response to viruses, and employed a highly controlled in vitro experimental system to clarify the point of action of lectin-mediated resistance in inhibiting virus infection. Based on these findings, we have elucidated the range of plant viruses in which lectin-mediated resistance is effective, and found that lectin -mediated resistance contributes to the control of viral diseases in practical crops.

Academic Significance and Societal Importance of the Research Achievements

植物免疫に関する基礎研究を実用的なウイルス耐性戦略に結びつけるためには、防御応答が具体的に病原体をどのように排除しているかの解明が最も重要であるが、技術的・材料的な制約からその作用点はほとんど解明されていない。本研究では植物ウイルスに対するレクチン抵抗性の作用点を解明し、さらにその知見を応用して実用作物において高度なウイルス抵抗性系統を作出した。これらの成果はこれまでブラックボックスであった植物免疫応答作用点の解明を通じて、新たな植物ウイルス耐性戦略確立に向けた知的・技術シーズを生み出すものであり、学術的・社会的意義が極めて高い。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (29 results)

All 2021 2020 2019 2018 2017

All Journal Article (9 results) (of which Peer Reviewed: 8 results,  Open Access: 5 results) Presentation (20 results) (of which Int'l Joint Research: 4 results,  Invited: 5 results)

  • [Journal Article] Complete genome sequence of pleioblastus mosaic virus, a distinct member of the genus Potyvirus.2021

    • Author(s)
      Katsu K., Nijo T., Yoshida T., Okano Y., Nishikawa M., Miyazaki A., Maejima K., Namba S., Yamaji Y
    • Journal Title

      Archives of Virology

      Volume: 166 Issue: 2 Pages: 645-649

    • DOI

      10.1007/s00705-020-04916-9

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 植物ウイルスに対する翻訳開始因子を介した劣性抵抗性2020

    • Author(s)
      藤本 祐司,橋本 将典,山次 康幸
    • Journal Title

      ウイルス

      Volume: 70 Pages: 61-68

    • NAID

      130008036170

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Interfamily transfer of Arabidopsis lectin-mediated antiviral gene confers resistance to pepino mosaic virus in tomato2020

    • Author(s)
      Okano Y., Maejima K., Yoshida T., Nishida S., Tokuda R., Nishikawa M., Namba S., Yamaji Y.
    • Journal Title

      Journal of General Plant Pathology

      Volume: in press Issue: 4 Pages: 274-282

    • DOI

      10.1007/s10327-020-00917-3

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transfection of Protoplasts Prepared from Arabidopsis thaliana Leaves for Plant Virus Research. Antiviral Resistance in Plants.2019

    • Author(s)
      Hosoe N., Keima T., Fujimoto Y., Hagiwara-Komoda Y., Hashimoto M., Maejima K., Namba S., Yamaji Y
    • Journal Title

      Methods Mol Biol

      Volume: 2028 Pages: 145-151

    • DOI

      10.1007/978-1-4939-9635-3_8

    • ISBN
      9781493996346, 9781493996353
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Complete Genome Sequence of a Carrot Torradovirus 1 Isolate, Obtained from Angelica keiskei in Japan2019

    • Author(s)
      Tokuda Ryosuke、Nishikawa Masanobu、Hosoe Naoi、Nijo Takamichi、Iwabuchi Nozomu、Yoshida Tetsuya、Watanabe Kiyoto、Maejima Kensaku、Yamaji Yasuyuki、Namba Shigetou
    • Journal Title

      Microbiology Resource Announcements

      Volume: 8 Issue: 15

    • DOI

      10.1128/mra.00110-19

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Complete Genome Sequence of Iris Severe Mosaic Virus Isolated in Japan2019

    • Author(s)
      Nishikawa Masanobu、Tokuda Ryosuke、Yoshida Tetsuya、Nijo Takamichi、Maruyama Noriko、Katsu Kosuke、Maejima Kensaku、Yamaji Yasuyuki、Namba Shigetou
    • Journal Title

      Microbiology Resource Announcements

      Volume: 8 Issue: 15

    • DOI

      10.1128/mra.00093-19

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The plant noncanonical antiviral resistance protein JAX1 inhibits potexviral replication by targeting the viral RNA-dependent RNA polymerase.2019

    • Author(s)
      Yoshida T., Shiraishi T., Hagiwara-Komoda Y., Komatsu K, Maejima K., Okano Y., Fujimoto Y., Yusa A., Yamaji Y., Namba S.
    • Journal Title

      Journal of Virology

      Volume: 93 Issue: 3

    • DOI

      10.1128/jvi.01506-18

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Complete genome sequence of lychnis mottle virus isolated in Japan2018

    • Author(s)
      Fujimoto Y., Nijo T., Hosoe N., Watanabe K., Maejima K., Yamaji Y., Namba S.
    • Journal Title

      Genome Announc.

      Volume: 6 Issue: 25

    • DOI

      10.1128/genomea.00535-18

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dual targeting of a virus movement protein to ER and plasma membrane subdomains is essential for plasmodesmata localization.2017

    • Author(s)
      Ishikawa K., Hashimoto M., Yusa A., Koinuma H., Kitazawa Y., Netsu O., Yamaji Y., Namba S.
    • Journal Title

      PLoS Pathogens

      Volume: 13 Issue: 6 Pages: e1006463-e1006463

    • DOI

      10.1371/journal.ppat.1006463

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 植物ウイルスの総合防除に向けた基礎研究2021

    • Author(s)
      山次 康幸
    • Organizer
      日本農学学会第46回大会シンポジウム
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] EXA1 を介した植物ウイルス抵抗性が 作用するウイルス効果範囲に関する 解 析2020

    • Author(s)
      藤本祐司・煉谷裕太朗・橋本将 典・吉田哲也・桂馬拓也・徳丸 海・前島健作・根津修 ・難波成任・山次康幸
    • Organizer
      令和2年度日本植物病理学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 植物で広く保存される劣性抵抗性遺伝 子EXA1 のポテックスウイルス感染に 対する普遍 性2020

    • Author(s)
      煉谷裕太朗・遊佐礼・橋本将典・吉田哲也・藤本祐司・前島健作・難波成任・山次康幸
    • Organizer
      令和2年度日本植物病理学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 抵抗性遺伝子JAX1 による広範なポ テックスウイルスの増殖阻害2020

    • Author(s)
      吉田哲也・岡野夕香里・西田萩子・ 松本旺樹・鈴木拓海・前島健作・難波 成任・山次康幸
    • Organizer
      令和2年度日本植物病理学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] pepino mosaic virus に抵抗性を示すJAX1 形質転換トマトの作出2020

    • Author(s)
      岡野夕香里・前島健作・吉田哲也・ 鈴木拓海・松本旺樹・難波成任・山次 康幸
    • Organizer
      令和2年度日本植物病理学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 抵抗性遺伝子から紐解く、植物とウイルスの共存戦略2020

    • Author(s)
      山次 康幸
    • Organizer
      第2回名古屋大学遺伝子実験施設公開セミナー
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Counter for the Epidemics of Potexviruses - Toward the Eradication of Pepino Mosaic Virus -2019

    • Author(s)
      Yamaji Y, Okano Y, Neriya Y, Hashimoto M, Yusa A, Hatanaka T, Narusaka Y, Yoshida T, Maejima K, Namba S
    • Organizer
      The 10th International Conference of Clinical Plant Science
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ウイルスを標的とした植物免疫研究とその応用展開2019

    • Author(s)
      山次康幸
    • Organizer
      第67回日本ウイルス学会学術集会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 東京都内のタケ亜科植物から検出されたポティウイルスの全ゲノム配列解析2019

    • Author(s)
      勝浩介・吉田哲也・丸山紀子・二條貴通・西川雅展・徳田遼佑・前島健作・難波成任・山次康幸
    • Organizer
      2019年日本植物病理学会関東部会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 国内のアシタバから検出されたcarrot torradovirus 1の全ゲノム配列2019

    • Author(s)
      徳田遼佑・西川雅展・細江尚唯・二條貴通・岩渕望・吉田哲也・渡邊清斗・前島健作・山次康幸・難波成任
    • Organizer
      平成31年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 国内で検出されたiris severe mosaic virusのゲノム配列の解析2019

    • Author(s)
      西川雅展・徳田遼佑・吉田哲也・二條貴通・丸山紀子・勝浩介・前島健作・山次康幸・難波成任
    • Organizer
      平成31年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] JAX1抵抗性の標的となるポテックスウイルス因子の解析2019

    • Author(s)
      勝浩介・遊佐礼・吉田哲也・白石拓也・小松健・橋本将典・前島健作・山次康幸・難波成任
    • Organizer
      平成31年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] JAX1によるポテックスウイルス増殖阻害のin vitro再構成2019

    • Author(s)
      藤本祐司・吉田哲也・白石拓也・薦田優香・小松健・岡野夕香里・前島健作・山次康幸・難波成任
    • Organizer
      平成31年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ポテックスウイルス複製複合体前駆体へのJAX1のターゲティング2019

    • Author(s)
      吉田哲也・薦田優香・藤本祐司・徳田遼佑・西川雅展・岡野夕香里・前島健作・山次康幸・難波成任
    • Organizer
      平成31年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Complete genome sequence of iris severe mosaic virus isolated in Japan2018

    • Author(s)
      Masanobu Nishikawa, Tetsuya Yoshida, Takamichi Nijo, Kensaku Maejima, Yasuyuki Yamaji and Shigetou Namba
    • Organizer
      International Conference of Clinical Plant Science 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of pepino mosaic virus2018

    • Author(s)
      Kosuke Katsu, Ryosuke Tokuda, Masanobu Nishikawa, Akio Miyazaki, Nozomu Iwabuchi, Kensaku Maejima, Yasuyuki Yamaji and Shigetou Namba
    • Organizer
      International Conference of Clinical Plant Science 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Complete genome sequence of carrot torradovirus 1 isolated from Angelica keiskei in Japan2018

    • Author(s)
      Ryosuke Tokuda, Masanobu Nishikawa, Kosuke Katsu, Naoi Hosoe, Nozomu Iwabuchi, Tetsuya Yoshida, Takamichi Nijo, Kensaku Maejima, Yasuyuki Yamaji, and Shigetou Namba
    • Organizer
      International Conference of Clinical Plant Science 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] イチジクモザイクウイルスの移行タンパク質が持つシグナルペプチドの機能解析2017

    • Author(s)
      細江尚唯 ・橋本将典 ・石川一也 ・北沢優悟 ・鯉沼宏章 ・藤本祐司 ・前島健作 ・山次康幸 ・難波成任
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] イチジクモザイクウイルスの移行タンパク質は異なる2 つのオルガネラを介してプラズモデスマータに輸送される2017

    • Author(s)
      橋本将典・細江尚唯 ・石川一也 ・北沢優悟 ・鯉沼宏章 ・藤本祐司 ・前島健作 ・山次康幸 ・難波成任
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 植物ウイルスに対する抵抗性機構とその利用2017

    • Author(s)
      山次康幸
    • Organizer
      平成29年度植物感染生理談話会
    • Related Report
      2017 Annual Research Report
    • Invited

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Published: 2017-04-28   Modified: 2022-01-27  

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