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Understanding the formation and function of apoplastic barriers

Research Project

Project/Area Number 17H03782
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionThe University of Tokyo

Principal Investigator

Kamiya Takehiro  東京大学, 大学院農学生命科学研究科(農学部), 准教授 (40579439)

Co-Investigator(Kenkyū-buntansha) 山上 睦  公益財団法人環境科学技術研究所, 環境影響研究部, 副主任研究員 (60715499)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2019: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2018: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2017: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywordsアポプラスト / カスパリー線 / スベリン / イオノーム
Outline of Final Research Achievements

The apoplastic barrier is the structure that separates the inside from the outside of the plant. Apoplastic barrier is composed of lignin (Casparian strip) and suberin composed of lipids. These are the cell wall structure of root endodermal and exodermal cells and form the barrier. However, the mechanism of their formation and their physiological roles remain largely unknown. In this study, using Arabidopsis thaliana, I analyzed genes directly regulated by MYB36, a master regulator of Casparian strip formation, and identified genes that play important functions in the subcellular localization of CASP1, a protein that serves as an important scaffold for Casparian strip formation. I also found that Casparian strip (apoplastic barrier) formation is regulated by low nutrition (potassium) and that this regulation is mediated by MYB36.

Academic Significance and Societal Importance of the Research Achievements

アポプラスト障壁は植物の内と外を分ける重要な構造であり、植物がさまざまな物質が含まれる土壌から、植物が必要なもののみを吸収するのに必要である。本研究では、これまでに同定したカスパリー線形成に重要な遺伝子(MYB36)を手がかりに、その形成機構の分子メカニズムの詳細を明らかにしたものである。加えて、栄養条件によってカスパリー線の形成が柔軟に変化することを示し、植物の環境適応応答について新しい知見を得ることができた。

Report

(5 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (19 results)

All 2022 2021 2019 2018

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 2 results) Presentation (11 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Book (1 results) Funded Workshop (2 results)

  • [Journal Article] CASP microdomain formation requires cross cell wall stabilization of domains and non-cell autonomous action of LOTR12022

    • Author(s)
      Kolbeck Andreas、Marhav? Peter、De Bellis Damien、Li Baohai、Kamiya Takehiro、Fujiwara Toru、Kalmbach Lothar、Geldner Niko
    • Journal Title

      eLife

      Volume: 11

    • DOI

      10.7554/elife.69602

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 1. Molecular mechanisms of Casparian strip and suberin lamella formation and their function in nutrient transport in roots2021

    • Author(s)
      神谷 岳洋
    • Journal Title

      Japanese Journal of Soil Science and Plant Nutrition

      Volume: 92 Issue: 2 Pages: 70-75

    • DOI

      10.20710/dojo.92.2_70

    • NAID

      130008024166

    • ISSN
      0029-0610, 2424-0583
    • Year and Date
      2021-04-05
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Novel Signaling Pathway Required for Arabidopsis Endodermal Root Organization Shapes the Rhizosphere Microbiome2021

    • Author(s)
      Durr Julius、Reyt Guilhem、Spaepen Stijn、Hilton Sally、Meehan Cathal、Qi Wu、Kamiya Takehiro、Flis Paulina、Dickinson Hugh G、Feher Attila、Shivshankar Umashankar、Pavagadhi Shruti、Swarup Sanjay、Salt David、Bending Gary D、Gutierrez-Marcos Jose
    • Journal Title

      Plant and Cell Physiology

      Volume: 62 Issue: 2 Pages: 248-261

    • DOI

      10.1093/pcp/pcaa170

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plasma membrane-associated Ca2+-binding protein PCaP1 is involved in root hydrotropism of Arabidopsis thaliana.2019

    • Author(s)
      Tanaka-Takada N, Kobayashi A, Takahashi H, Kamiya T, Kinoshita T, Maeshima M.
    • Journal Title

      Plant & Cell Physiology

      Volume: - Issue: 6 Pages: 0-0

    • DOI

      10.1093/pcp/pcz042

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Local boron concentrations in tuberous roots of Japanese radish (Raphanus sativus L.) negatively correlate with distribution of brown heart2018

    • Author(s)
      Sotta, N., Bian, B., Peng, D., Hongkham, P., Kamiya, T., Niikura, S. and Fujiwara, T.
    • Journal Title

      Plant Physiology and Biochemistry

      Volume: 136 Pages: 58-66

    • DOI

      10.1016/j.plaphy.2018.12.027

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Two kinase regulated by MYB36 are essential for correct localization of lignin in Casparian strip domain2019

    • Author(s)
      Wu Qi, Takehiro Kamiya
    • Organizer
      4th Int'l Symposium on Plant Apoplastic Diffusion
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 植物の根における栄養吸収 ―植物の内と外を分ける内皮の物質輸送障壁の形成と機能―2019

    • Author(s)
      神谷岳洋
    • Organizer
      第4回トランスポーター研究会関東部会(JTRAKB2019)
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Endodermal cell differentiation and apoplastic barrier formation2019

    • Author(s)
      Takehiro Kamiya
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Two kinases regulated by MYB36 are essential for correct localization of lignin deposition in the Casparian strip domain2019

    • Author(s)
      Wu Qi, Toru Fujiwara, Takehiro Kamiya
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Golgi-localized OsFPN1 is required for cobalt and nickel homeostasis in rice2019

    • Author(s)
      Manman Kan, Toru Fujiwara, Takehiro Kamiya
    • Organizer
      第60回 日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Coordinated formation of apoplastic barrier: Cas parian strips and suberin lamellae2018

    • Author(s)
      Takehiro Kamiya
    • Organizer
      Biosynthesis and function of suberin
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シロイヌナズナのカロース合成酵素遺伝子の低Ca耐性における作用部位と作用様式の解析2018

    • Author(s)
      鹿内勇佑、山上睦、重信秀治、山口勝司、神谷岳洋、藤原徹
    • Organizer
      日本土壌肥料学会2018年度神奈川大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Low Magnesium Tolerance 1 physically associates with ER-localized Mg transporters and plays an important role in extending Mg stress adaptation range in Arabidopsis thaliana2018

    • Author(s)
      Zhihang Feng, Takehiro Kamiya, Toru Fujiwara
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] シロイヌナズナにおける低 Ca 耐性への GSLファミリー遺伝子の寄与の解析2018

    • Author(s)
      鹿内勇佑,淺田真由,黒木俊作,山上睦,重信秀治,山口勝司,神谷岳洋,藤原徹
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Coordinated formation of apoplastic barrier: Casparian strips and suberin lamellae2018

    • Author(s)
      Takehiro Kamiya
    • Organizer
      Biosynthesis and function of suberin
    • Related Report
      2017 Annual Research Report
  • [Presentation] Isolation and characterization of the rice mutant with high molybdenum in grain2018

    • Author(s)
      Prashant Kandwal, 田中 伸裕, 藤原 徹, 神谷 岳洋
    • Organizer
      日本土壌肥料学会 2018年度神奈川大会
    • Related Report
      2017 Annual Research Report
  • [Book] Plant Micronutrient Use Efficiency2018

    • Author(s)
      Mohammad Anwar Hossain, Takehiro Kamiya, David Burritt, Lam-Son Phan Tran, Toru Fujiwara
    • Total Pages
      324
    • Publisher
      Elsevier
    • Related Report
      2017 Annual Research Report
  • [Funded Workshop] Biosynthesis and function of suberin2018

    • Related Report
      2018 Annual Research Report
  • [Funded Workshop] Biosynthesis and function of suberin2018

    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2023-01-30  

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