| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2019: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2018: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Outline of Final Research Achievements |
Ideonella sakaiensis is capable of growing on PET as a carbon nutrition. In this study, we explored the mechanism of efficient PET degradation by I. sakaiensis and found things as follows. (1) Mutations were introduced into the substrate binding site of PETase, and it was found that the high PET substrate specificity of PETase is greatly influenced by these amino acid residues. (2) We succeeded in constructing a gene disruption system for I. sakaiensis, and created Δpetase and Δmhetase strains. (3) Proteomic analysis revealed an enzyme with hydrolytic activity against PET oligomers.
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