| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Outline of Final Research Achievements |
We analyzed the mechanism of preferential translation under high concentrations of ethanol stress and found that RNA-binding proteins such as Rbp26 were essential for the preferential translation of BTN2. We also analyzed mRNAs that bind to Rbp26 under high concentrations of ethanol stress and identified new genes that are preferentially translated in a similar manner to BTN2. Furthermore, we found that the preferentially translated genes varied with stress treatment time. Analysis of Rbp26-binding mRNAs allowed us to efficiently identify preferentially translated genes and to understand their variation over time. In this study, we succeeded in establishing an effective experimental method for gene expression under translational repression by high concentrations of ethanol.
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