| Budget Amount *help |
¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
Fiscal Year 2020: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2019: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2018: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
|
|---|
| Outline of Final Research Achievements |
We have been conducting computational chemistry focusing on the untranslated regions responsible for the regulation of PGA synthesis gene expression in Bacillus megaterium and Bacillus natto, and B. megaterium has a special nucleotide sequence harboring a riboswitch-like structure, which is adjacent to the the global regulator (DegU)-recognition site. An inhibitory-PCR method was hence devised to examine the effects of rare earths on higher functions and structures of the regulatory region (Cap/5'-UTR) (nullification of the attenuation mechanism, selectivity for heavy rare earth elements, and so on). DegU was analyzed by "gel shift assay". As a result, it was found that the combination of the abiotic metal species (Dy) and the post-translationally modified protein (DegU-P) controls the binding behavior with a specific DNA sequence.
|
