• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Further development of megalosaccharide research: synthesis and application of novel megalosaccharides displaying excellent functions

Research Project

Project/Area Number 17H03801
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionHokkaido University

Principal Investigator

KIMURA Atsuo  北海道大学, 農学研究院, 教授 (90186312)

Co-Investigator(Kenkyū-buntansha) 田上 貴祥  北海道大学, 農学研究院, 助教 (70709849)
奥山 正幸  北海道大学, 農学研究院, 講師 (00344490)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Keywordsメガロ糖 / 機能解析 / 可溶化
Outline of Final Research Achievements

We succeeded in production of megalosaccharides and found their valuable function to solubilize water-insoluble compounds. However, the period of our research is very short, so that we have many problems that must be solved. This project challenges a resolution of three important problems, from which we will obtain the fundamental knowledge about megalosaccharides. Furthermore, it also contributes to the development of application research on megalosaccharides. The purposes of this program are 1) analysis of molecular mechanism of polysaccharide-forming enzyme to produce megalosaccharide, 2) synthesis of new megalosaccharide with high functionality, and 3) improvement of azo-dye pollution using megalosaccharide.

Academic Significance and Societal Importance of the Research Achievements

本研究成果の学術的意義として、多糖合成酵素において、多糖やメガロ糖の生産に関与する部分領域を推定し、その責任アミノ酸を同定することで、合成機構を新たに提案した。アミラーゼ耐性メガロ糖やグルコース型メガロ糖を新しく作製し、これらの糖質にも難溶性化合物の水溶化現象を見出した。本研究から難溶な食品素材の可溶化・アゾ色素の分解などのメガロ糖を活用する応用技術が考案され、社会貢献を実現できる成果が得られた。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (34 results)

All 2021 2020 2019 2018 2017 Other

All Int'l Joint Research (4 results) Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (22 results) (of which Int'l Joint Research: 5 results,  Invited: 2 results) Remarks (3 results)

  • [Int'l Joint Research] マハサラカーン大学(タイ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] マハサラカーン大学(タイ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] マハサラカーン大学(タイ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] マハサラカーン大学(タイ)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Molecular insight into regioselectivity of transfructosylation catalyzed by GH68 levansucrase and β-fructofuranosidase2021

    • Author(s)
      Okuyama Masayuki、Serizawa Ryo、Tanuma Masanari、Kikuchi Asako、Sadahiro Juri、Tagami Takayoshi、Lang Weeranuch、Kimura Atsuo
    • Journal Title

      Journal of Biological Chemistry

      Volume: 296 Pages: 100398-100398

    • DOI

      10.1016/j.jbc.2021.100398

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A novel intracellular dextranase derived from Paenibacillus sp. 598K with an ability to degrade cycloisomaltooligosaccharides.2019

    • Author(s)
      Mizushima D, Miyazaki T, Shiwa Y, Kimura K, Suzuki S, Fujita N, Yoshikawa H, Kimura A, Kitamura S, Hara H, Funane K
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 103 Issue: 16 Pages: 6581-6592

    • DOI

      10.1007/s00253-019-09965-y

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] α-1,3/α-1,4-Glucosidase from Aspergillus niger Exhibits Unique Transglucosylation to Generate High Levels of Nigerose and Kojibiose.2019

    • Author(s)
      Ma M, Okuyama M, Tagami T, Kikuchi A, Klahan P, Kimura A.
    • Journal Title

      J Agric Food Chem

      Volume: 67 Issue: 12 Pages: 3380-3388

    • DOI

      10.1021/acs.jafc.8b07087

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Engineered dextranase from Streptococcus mutans enhances the production of longer isomaltooligosaccharides.2018

    • Author(s)
      Klahan P, Okuyama M, Jinnai K, Ma M, Kikuchi A, Kumagai Y, Tagami T, Kimura A.
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 82 Issue: 9 Pages: 1480-1487

    • DOI

      10.1080/09168451.2018.1473026

    • NAID

      120006633283

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Enzymatically synthesized megalo-type isomaltosaccharides enhance the barrier function of the tight junction in the intestinal epithelium2018

    • Author(s)
      Hara Hiroshi、Kume Shunsuke、Iizuka Takahisa、Fujimoto Yoshinori、Kimura Atsuo
    • Journal Title

      Biosci Biotechnol Biochem.

      Volume: 82 Issue: 4 Pages: 629-635

    • DOI

      10.1080/09168451.2017.1398065

    • NAID

      120006540057

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] α-グルコシダーゼによるα-マンノシル基転移反応の受容体特異性の解析2021

    • Author(s)
      藤井大河,床波 篤,田上貴祥,奥山正幸,木村淳夫
    • Organizer
      日本応用糖質科学会北海道支部会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 糸状菌α-グルコシダーゼBへの部位特異的飽和変異導入が生成物特異性に与える影響2020

    • Author(s)
      床波篤,Min Ma,奥山正幸,田上貴祥,木村淳夫
    • Organizer
      日本農芸化学会北海道支部会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Aspergillus niger α-glucosidase Bの機能解析.2020

    • Author(s)
      床波 篤,Min Ma,奥山正幸,田上貴祥,木村淳夫
    • Organizer
      日本応用糖質科学会北海道支部会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 放線菌Kribbella flavida由来 α-glucosidaseの機能解析.2020

    • Author(s)
      中山英里香,田上貴祥,奥山正幸,木村淳夫
    • Organizer
      日本応用糖質科学会北海道支部会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 放線菌由来GH13_30 α-グルコシダーゼの機能解析.2020

    • Author(s)
      佐分利亘、田上貴祥、壺井芙美、奥山正幸、木村淳夫、森春英
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ニゲロース特異的なSolute-binding proteinの発見.2020

    • Author(s)
      田上貴祥、壷井芙美、佐藤宏樹、奥山正幸、佐分利亘、森春英、木村淳夫
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] α-グルコシダーゼを用いたα-マンノシルオリゴ糖の合成.2020

    • Author(s)
      前山和輝、田上貴祥、奥山正幸、木村淳夫
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ブタ血清由来マルターゼ-グルコアミラーゼを構成する2つのサブユニットは独立に機能する.2020

    • Author(s)
      渡邊憲、田上貴祥、奥山正幸、木村淳夫
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Five function-unknown GH97 paralogs from Bacteroides thetaiotaomicron.2019

    • Author(s)
      Kikuchi A, Nakagawa Y, Okuyama M, Tagami T, Kimura A
    • Organizer
      13th Carbohydrate Bioengineering Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] GH97 α-glucoside hydrolases in Bacteroides thetaiotaomicron.2019

    • Author(s)
      Kikuchi A, Okuyama M, Tagami T, Kimura A
    • Organizer
      7th Symposium on the Alpha-Amylase Family
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] デキストランデキストリナーゼのSer923の機能解析.2019

    • Author(s)
      嶌村有季乃,田上貴祥,奥山正幸,木村淳夫
    • Organizer
      日本応用糖質科学会2019年度大会(第69回)
    • Related Report
      2019 Annual Research Report
  • [Presentation] β-Fructofuranosidaseの糖転移作用:飽和変異導入による改変.2019

    • Author(s)
      髙木惇生、奥山正幸、田上貴祥、木村淳夫
    • Organizer
      日本応用糖質科学会2019年度大会(第69回)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Parageobacillus caldoxylosilyticusの糖質加水分解酵素:機能解明と糖転移向上2018

    • Author(s)
      陣内恒平、吉田強軌、奥山正幸、田上貴祥、木村淳夫
    • Organizer
      日本農芸化学会北海道支部
    • Related Report
      2018 Annual Research Report
  • [Presentation] 改変β-fructofuranosidaseによる6-ケストースの合成および精製2018

    • Author(s)
      髙木惇生、芹沢領、奥山正幸、田上貴祥、志村華子、上野敬司、小野寺秀一、木村淳夫
    • Organizer
      日本農芸化学会東北・北海道支部
    • Related Report
      2018 Annual Research Report
  • [Presentation] ブタ血清由来α-グルコシダーゼのcDNAクローニングと組換え酵素の機能解析2018

    • Author(s)
      渡邊憲、田上貴祥、奥山正幸、木村淳夫
    • Organizer
      日本農芸化学会東北・北海道支部
    • Related Report
      2018 Annual Research Report
  • [Presentation] Structural and functional investigation of starch-metabolizing enzymes2018

    • Author(s)
      Tagami T, Okuyama M, Mori H, Kimura A
    • Organizer
      Protein Society of Thailand
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular characterization of glycosidases in GH97, subfamily c2018

    • Author(s)
      Kikuchi A, Oyama M, Kato K, Tagami T, Yao M, Kimura A
    • Organizer
      International Carbohydrate Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular mechanism of transglucosylation catalyzed by two α-glucosidase isozymes from Aspergillus niger2017

    • Author(s)
      Min Ma, Masayuki Okuyama, Takayoshi Tagami, Patcharapa Klahan, Atsuo Kimura
    • Organizer
      Asia Pacific Protein Association and Protein Society of Thailand
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 小腸上皮バリア機能に対するアンカー型イソマルトメガロ糖の保護作用2017

    • Author(s)
      白戸しおり, 飯塚貴久,藤本佳則,舟根和美,木村淳夫,原博
    • Organizer
      日本応用糖質科学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] GH15 isomaltose glucohydrolaseのX線結晶構造解析:基質認識に関わる構造因子の推定2017

    • Author(s)
      田上貴祥, 陳明皓, 奥山正幸, 岩﨑智仁, 田中良和, 姚閔, 木村淳夫
    • Organizer
      日本応用糖質科学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] デキストラン デキストリナーゼの多糖合成に関与するアミノ酸の同定2017

    • Author(s)
      佐々木優希、熊谷祐也、貞廣樹里、ラング ヴィーラヌッチ、田上貴祥、奥山正幸、木村淳夫
    • Organizer
      日本農芸化学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Down-regulation of Cd14 expression in liver by ingestion of megalo-type isomaltosaccharides might be prerequisite for amelioration of LPS-induced liver injury in rats2017

    • Author(s)
      Ga Hyun Joe, Takahisa Iizuka, Yoshinori Fujimoto, Atsuo Kimura, Hiroshi Hara, Satoshi Ishizuka
    • Organizer
      日本農芸化学会
    • Related Report
      2017 Annual Research Report
  • [Remarks] 分子酵素学研究室 北海道大学 大学院農学研究院 応用生命科学分野

    • URL

      http://lab.agr.hokudai.ac.jp/molenzlab/

    • Related Report
      2020 Annual Research Report
  • [Remarks] 分子酵素学研究室 北海道大学 大学院農学研究院 応用生命科学分野

    • URL

      https://lab.agr.hokudai.ac.jp/molenzlab/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 分子酵素学研究室 | 北海道大学大学院 農学研究院 - 北海道大学 農学部

    • URL

      https://lab.agr.hokudai.ac.jp/molenzlab/

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi