| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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| Outline of Final Research Achievements |
Plants have a great ability to synthesis ascorbate known as vitamin C in response to light. In this study, to understand the molecular mechanism of light-inducible ascorbate biosynthesis, we carried out gene and protein expression analysis and obtained the following conclusion. 1) We generate novel Arabidopsis mutants showing abnormal VTC2 gene expression pattern in response to light, and analyzed their causal genes. 2) One of key regulation of light-inducible ascorbate biosynthesis is phosphorylation of some proteins via VTC3, a novel enzyme, and its down-stream target factor would be VTC2. Actually, recombinant VTC2 analysis showed that phosphorylation of affect significantly its enzymatic stability.
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