| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
Seven novel bicyclic guanidino compounds were isolated from the tetrodotoxin (TTX) possessing pufferfish, and their structures were elucidated. These compounds have the same relative stereochemistry as that of TTX. Based on their structures, oxidative biosynthetic pathway towards TTX was suggested. For biosynthesis of saxitoxin (STX), predicted intermediates, 11-hydroxy Int-C’2 and Int-E’were chemically synthesized. They were identified in toxin producing dinoflagellate and cyanobacterium using HR-LC/MS. In addition, a new STX analogue, 12beta-deoxyGTX3 was identified in the toxic cyanobacterium using the standard which was prepared by chemical derivatization.
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