Regulation mechanisms of the momilactone gene cluster and its evolution in momilactone producing plantss
| Project/Area Number |
17H03811
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioorganic chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Okada Kazunori 東京大学, 生物生産工学研究センター, 准教授 (20312241)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
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| Keywords | 二次代謝物質 / 生合成遺伝子クラスター / 転写制御 / 病害抵抗性 / テルペノイド / モミラクトンＡ遺伝子クラスター / 蘚類ハイゴケ / 転写因子 / ゲラニルゲラニル２リン酸 / ファイトアレキシン / 遺伝子クラスター / モミラクトン / オキシリピン / 転写制御因子 / ハイゴケ / 野生イネ / アレロパシー / 植物成長調節物質 |
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| Outline of Final Research Achievements |
The cultivated rice Oryza sativa has shown to hold two biosynthetic gene clusters on their genome for the production of antimicrobial diterpenoid phytoalexins. In this study, we first showed in planta functions of P450 oxygenases that comprise the biosynthetic pathway of ditepenoid phytoalexins in cultivated rice by analyzing metabolic flows in the pathway using a feeding approach with stable isotopes. We also analyzed two major transcriptional factors involved in the regulation of the genes in the diterpenoid phytoalexin biosynthetic gene clusters and demonstrated that OsTAGP1 has an important role on an allelopathic activity in roots and DPF is both genetically and functionally conserved in wild-rice. Finally, we discovered a functional biosynthetic gene cluster for momilactone A in bryophyte Calohypnum plumiforme, and suggested a genomic evidence for convergent evolution of the momilactone biosynthetic gene cluster in rice, barnyardgrass, and the moss.
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| Academic Significance and Societal Importance of the Research Achievements |
植物に競争力を与えるモミラクトンはこれまでイネだけが利用していると考えられてきたが、イヌビエやハイゴケなど複数の進化的に離れた植物においても、ゲノム上の遺伝子クラスターの存在と共にモミラクトン合成能を維持していることが明らかになり、陸上植物が利用可能な生理活性物質としてのポテンシャルが高いことが示された。さらに、ハイゴケのモミラクトン生合成遺伝子クラスター内の遺伝子を用い、異種植物におけるモミラクトン生産にも成功した。これらの成果を利用することで、モミラクトンを生産し病害抵抗性を付与した新たな作物の開発につながる期待が大きく、本研究成果は学術的に重要なだけでなく社会的意義を持つものである。
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Report
(4 results)
Research Products
(36 results)
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[Journal Article] Genomic evidence for convergent evolution of gene clusters for momilactone biosynthesis in land plants2020
Author(s)
Mao L, Kawaide H, Higuchi T, Chen M, Miyamoto K, Hirata Y, Kimura H, Miyazaki S, Teruya M, Fujiwara K, Tomita K, Yamane H, Hayashi K, Nojiri H, Jia L, Qiu J, Ye C, Timko MP, Fan L, and Okada K
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Journal Title
Proc Natl Acad Sci USA
Volume: Online ahead of print
Issue: 22
Pages: 12472-12480
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Expression of RSOsPR10 in rice roots is antagonistically regulated by jasmonate/ethylene and salicylic acid via the activator OsERF87 and the repressor OsWRKY76, respectively2018
Author(s)
Yamamoto, T., Yoshida, Y., Nakajima, K., Tominaga, M., Gyohda, A., Suzuki, H., Okamoto, T., Nishimura, T., Yokotani, N., Minami, E., Nishizawa, Y., Miyamoto, K., Yamane, H., Okada, K., Koshib, T.
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Journal Title
Plant Direct
Volume: ３
Issue: 3
Pages: 1-17
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Barnyardgrass (Echinochloa crus-galli) genome analysis provides insight into its adaptation and invasiveness as a weed.2017
Author(s)
Guo L, Qiu J, Ye C, Jin G, Mao L, Zhang H, Yang X, Peng Q, Wang Y, Jia L, Lin Z, Li G, Fu F, Liu C, Chen L, Aoki D, Yumoto E, Yokota T, Miyamoto M, Okada K, Kim DC, Cai D, Zhang C, Lou Y, Qian Q, Yamaguchi H, Yamane H, Kong CH,Timko MP, Bai L, Fan L et al.,
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Journal Title
Nature Communications
Volume: 8(1)
Issue: 1
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] An evolutionary point of view of the biosynthetic gene clusters formation for phytoalexins in rice and related species.2017
Author(s)
Shiho Tomiyama, Ryouka Kawahara-Miki, Kaihei Koshio, Koji Miyamoto, Hisakazu Yamane, Jie Qiu, Longjiang Fan, Tomonobu Toyomasu, Nori Kurata, Hideaki Nojiri, Kazunori Okada
Organizer
ISRFG 2017
Related Report
Int'l Joint Research
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