| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2017: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Outline of Final Research Achievements |
We found that indigestible oligosaccharides increase the expression of a group of microRNAs (miRNAs) in colonic lamina propria lymphocytes of mice. Many of these miRNAs were consistent with miRNAs whose expression levels were higher in conventional mice than in germ-free mice. These findings suggest that miRNA-induced gene silencing is involved in the gut microbiota influence on gut immune homeostasis. Additionally, by transfecting the miRNA mimics to a human T-cell line Jurkat, we established an experimental system to search for target genes and to examine the effect on T-cell differentiation.
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