Creation of Innovative Presentation and Prediction Methodology of Food Factor Combination for Regulating the Medical Redox State

• Project/Area Number: 17H03819
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Kyushu University
• Principal Investigator: Fujimura Yoshinori 九州大学, 農学研究院, 准教授 (20390304)
• Co-Investigator(Kenkyū-buntansha): 兵藤 文紀 岐阜大学, 大学院医学系研究科, 特任准教授 (10380693) ; 瀬戸山 大樹 九州大学, 大学病院, 助教 (30550850) ; 三浦 大典 九州大学, 農学研究院, 特任准教授 (40532627)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: メタボリック・プロファイリング / 抗酸化活性 / レドックス / 質量分析 / MRI / ポリフェノール / 標的分子 / ケモメトリクス / イメージング / DNP-MRI / 抗酸化性 / 生体磁気共鳴解析 / 代謝物解析 / ミトコンドリア / がん代謝マーカー / 生体磁気共鳴画像解析

Outline of Final Research Achievements

Reduction-oxidation (redox) reaction is basis of life phenomena, and it may become favorable targets for health-promoting effect and disease risk-reducing effect of foods. Redox reaction has also received an attractive attention as combination effects in multi-component food systems. In this study, we developed the visualizing technique of biomedical redox state useful for evaluating anti-oxidative activity. We also succeeded in creating new composition-evaluating chemometrics for theoretically and precisely determining combination of food ingredients capable of regulating redox reactions.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は、食品成分・素材の抗酸化活性に着目した組合せ効果の理解（科学的根拠の提示）や予測、成分の有効活用に資する機能性食品の戦略的立案や食べ合わせに役立つ成分評価のブレークスルー技術を提案するものである。また、生体レドックス可視化技術は、食品成分の抗酸化活性の高精度評価ならびに酸化ストレス応答に関連する食品感受性マーカーや疾病発症リスクマーカー開発に寄与する技術的イノベーションにつながることが期待できる。

Research Products

• [Journal Article] Cancer cell selective probe by mimicking EGCG (2020)
  • Author(s): Kumazoe Motofumi、Hiroi Shun、Tanimoto Yousuke、Miyakawa Jyunichi、Yamanouchi Maasa、Suemasu Yumi、Yoshitomi Ren、Murata Motoki、Fujimura Yoshinori、Takahashi Takashi、Tanaka Hiroshi、Tachibana Hirofumi
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 525 Issue: 4 Pages: 974-981
  • DOI: 10.1016/j.bbrc.2020.03.021
  • Peer Reviewed / Open Access
• [Journal Article] Procyanidin C1 Inhibits Melanoma Cell Growth by Activating 67‐kDa Laminin Receptor Signaling (2020)
  • Author(s): Bae Jaehoon、Kumazoe Motofumi、Murata Kyosuke、Fujimura Yoshinori、Tachibana Hirofumi
  • Journal Title: Molecular Nutrition & Food Research Volume: - Issue: 7 Pages: 1900986-1900986
  • DOI: 10.1002/mnfr.201900986
  • Peer Reviewed
• [Journal Article] Epigallocatechin-3-O-gallate induces acid sphingomyelinase activation through activation of phospholipase C (2019)
  • Author(s): Bae Jaehoon、Kumazoe Motofumi、Takeuchi Chieri、Hidaka Shiori、Fujimura Yoshinori、Tachibana Hirofumi
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 520 Issue: 1 Pages: 186-191
  • DOI: 10.1016/j.bbrc.2019.09.102
  • Peer Reviewed / Open Access
• [Journal Article] Cuprizone-treated mice, a possible model of schizophrenia, highlighting the simultaneous abnormalities of GABA, serine and glycine in hippocampus (2019)
  • Author(s): Hayakawa Eisuke、Ohgidani Masahiro、Fujimura Yoshinori、Kanba Shigenobu、Miura Daisuke、Kato Takahiro A.
  • Journal Title: Schizophrenia Research Volume: 210 Pages: 326-328
  • DOI: 10.1016/j.schres.2019.06.010
  • Peer Reviewed
• [Journal Article] Improvement of Sensitivity and Reproducibility for Imaging of Endogenous Metabolites by Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry. (2019)
  • Author(s): Morikawa-Ichinose T, Fujimura Y, Murayama F, Yamazaki Y, Yamamoto T, Wariishi H, Miura D.
  • Journal Title: J. Am. Soc. Mass Spectrom. Volume: 30 Issue: 8 Pages: 1512-1520
  • DOI: 10.1007/s13361-019-02221-7
  • Peer Reviewed
• [Journal Article] In vivo melanoma imaging based on dynamic nuclear polarization enhancement in melanin pigment of living mice using in vivo dynamic nuclear polarization magnetic resonance imaging (2019)
  • Author(s): Hyodo Fuminori、Naganuma Tatsuya、Eto Hinako、Murata Masaharu、Utsumi Hideo、Matsuo Masayuki
  • Journal Title: Free Radical Biology and Medicine Volume: 134 Pages: 99-105
  • DOI: 10.1016/j.freeradbiomed.2019.01.002
  • Peer Reviewed
• [Journal Article] Development and validation of the 22-item Tarumi's modern-type depression trait scale; Avoidance of social roles, Complaint and low Self-esteem (TACS-22) (2019)
  • Author(s): Kato TA, Katsuki R, Kubo H, Shimokawa N, Sato-Kasai M, Hayakawa K, Kuwano N, Umene-akano W, Tateno M, Setoyama D, Kang D, Watabe M, Sakamoto S, Teo AR, Kanba S
  • Journal Title: Psychiatry and clinical neurosciences Volume: in press Issue: 8 Pages: 448-457
  • DOI: 10.1111/pcn.12842
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Diallyl disulfide potentiates anti-obesity effect of green tea in high-fat/high-sucrose diet-induced obesity (2019)
  • Author(s): Bae Jaehoon、Kumazoe Motofumi、Fujimura Yoshinori、Tachibana Hirofumi
  • Journal Title: The Journal of Nutritional Biochemistry Volume: 64 Pages: 152-161
  • DOI: 10.1016/j.jnutbio.2018.10.014
  • Peer Reviewed
• [Journal Article] Postprandial glycaemia-lowering effect of a green tea cultivar Sunrouge and cultivar-specific metabolic profiling for determining bioactivity-related ingredients (2018)
  • Author(s): Masafumi Wasai, Yoshinori Fujimura, Haruna Nonaka, Ryo Kitamura, Motoki Murata, and Hirofumi Tachibana
  • Journal Title: Sci. Rep. Volume: 8 Issue: 1 Pages: 16041-16041
  • DOI: 10.1038/s41598-018-34316-8
  • Peer Reviewed / Open Access
• [Journal Article] Visualizing Energy Charge in Breast Carcinoma Tissues by MALDI Mass-spectrometry Imaging Profiles of Low-molecular-weight Metabolites (2018)
  • Author(s): TORATA NOBUHIRO、KUBO MAKOTO、MIURA DAISUKE、OHUCHIDA KENOKI、MIZUUCHI YUSUKE、FUJIMURA YOSHINORI、HAYAKAWA EISUKE、KAI MASAYA、ODA YOSHINAO、MIZUMOTO KAZUHIRO、HASHIZUME MAKOTO、NAKAMURA MASAFUMI
  • Journal Title: Anticancer Research Volume: 38 Issue: 7 Pages: 4267-4272
  • DOI: 10.21873/anticanres.12723
  • Peer Reviewed
• [Journal Article] Mitochondrial p32/C1qbp Is a Critical Regulator of Dendritic Cell Metabolism and Maturation (2018)
  • Author(s): Gotoh Kazuhito、Morisaki Takafumi、Setoyama Daiki、Sasaki Katsuhiko、Yagi Mikako、Igami Ko、Mizuguchi Soichi、Uchiumi Takeshi、Fukui Yoshinori、Kang Dongchon
  • Journal Title: Cell Reports Volume: 25 Issue: 7 Pages: 1800-1815
  • DOI: 10.1016/j.celrep.2018.10.057
  • Peer Reviewed / Open Access
• [Journal Article] Photoinduced in Vivo Magnetic Resonance Imaging (MRI) with Rapid CO Release from an MnCO-Protein Needle Composite (2018)
  • Author(s): Hyodo Fuminori、Sho Takeya、Maity Basudev、Fujita Kenta、Tachibana Yoko、Akashi Satoko、Mano Megumi、Hishikawa Yuki、Matsuo Masayuki、Ueno Takafumi
  • Journal Title: Chemistry - A European Journal Volume: 24 Issue: 45 Pages: 11578-11583
  • DOI: 10.1002/chem.201802445
  • Peer Reviewed
• [Journal Article] Analysis of spatiotemporal metabolomic dynamics for sensitively monitoring biological alterations in cisplatin-induced acute kidney injury. (2018)
  • Author(s): Irie, M., Hayakawa, E., Fujimura, Y., Honda, Y., Setoyama, D., Wariishi, H., Hyodo, F. and Miura, D.*
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 496 Issue: 1 Pages: 140-146
  • DOI: 10.1016/j.bbrc.2018.01.012
  • Peer Reviewed
• [Journal Article] A Phytochemical-Sensing Strategy Based on Mass Spectrometry Imaging and Metabolic Profiling for Understanding the Functionality of the Medicinal Herb Green Tea. (2017)
  • Author(s): Fujimura Y, Miura D, Tachibana H.
  • Journal Title: Molecules Volume: 22 Issue: 10 Pages: 1621-1621
  • DOI: 10.3390/molecules22101621
  • Peer Reviewed / Open Access
• [Journal Article] A Chemometrics-driven Strategy for the Bioactivity Evaluation of Complex Multicomponent Systems and the Effective Selection of Bioactivity-predictive Chemical Combinations. (2017)
  • Author(s): Fujimura, Y., Kawano, C., Maeda-Murayama, A., Nakamura, A., Koike-Miki, A., Yukihira, D., Hayakawa, E., Ishii, T., Tachibana, H., Wariishi, H. and Miura, D.*
  • Journal Title: Sci. Rep. Volume: 7 Issue: 1 Pages: 2257-2257
  • DOI: 10.1038/s41598-017-02499-1
  • Peer Reviewed / Open Access
• [Presentation] サンルージュの食後血糖上昇抑制作用とその関与成分の同定. (2020)
  • Author(s): 渡邉 喬嗣, 和才 昌史, 藤村 由紀, 三浦 大典, 立花 宏文.
  • Organizer: 日本農芸化学会2020年度大会
• [Presentation] Metabolic profiling for the bioactivity evaluation of green tea and the selection of bioactivity-related chemical combinations. (2019)
  • Author(s): Fujimura Y, Miura D, and Tachibana H.
  • Organizer: The 9th International Conference on Polyphenols and Health (ICPH2019)
  • Int'l Joint Research / Invited
• [Presentation] 機能性フードペアリングのABC (2019)
  • Author(s): 藤村 由紀, 三浦 大典, 立花 宏文
  • Organizer: JASISコンファレンス ライフサイエンスイノベーションフォーラムI
  • Invited
• [Presentation] Development of sample preparation method to improve sensitivity and reproducibility of mass spectrometry imaging of endogenous metabolites. (2019)
  • Author(s): Morikawa-Ichinose, T, Fujimura, Y, Murayama, F, Yamazaki, Y, Yamamoto, T, Wariishi, H, Miura, D.
  • Organizer: 67th ASMS Conference on Mass Spectrometry and Allied Topics
  • Int'l Joint Research
• [Presentation] 高感度かつ再現性の高い代謝物分布解析を可能にする質量分析イメージング試料調製法の開発. (2019)
  • Author(s): 一瀬 智美, 藤村 由紀, 村山 布紗, 山崎 雄三, 山本 卓志, 割石 博之, 三浦 大典.
  • Organizer: 第６７回質量分析総合討論会
• [Presentation] メチル化カテキンのこうアレルギー作用に対するエリオジクチオールの増強作用. (2019)
  • Author(s): 藤野 小夏, 吉本 孝憲, 藤村 由紀, 立花 宏文.
  • Organizer: 2019年度日本栄養・食糧学会九州・沖縄支部および日本食品科学工学会西日本支部合同大会
• [Presentation] 緑茶の機能性を多面的に捉えるメタボリック・プロファイリング (2019)
  • Author(s): 藤村 由紀, 三浦 大典, 立花 宏文
  • Organizer: 日本農芸化学会2019年大会
  • Invited
• [Presentation] Metabolic Profiling Strategy for the Bioactivity Evaluation of Green Tea and the Selection of Bioactivity-related Chemical Combinations. (2019)
  • Author(s): Fujimura Y, Miura D, and Tachibana H.
  • Organizer: The 3rd Symposium of Kyoto Biomolecular Mass Spectrometry Society
  • Int'l Joint Research / Invited
• [Presentation] 質量分析イメージング技術の開発と食品科学分野への応用 (2018)
  • Author(s): 藤村 由紀.
  • Organizer: 第一回プロテオミクス先端技術研究会
  • Invited
• [Presentation] 生体レドックスに及ぼす緑茶ポリフェノールの影響 (2018)
  • Author(s): 藤村 由紀, 兵藤 文紀, 立花 宏文.
  • Organizer: 2018年度（第22回）生物機能研究会
  • Invited
• [Presentation] ニュートリメタボロミクスを切り拓く複合成分系食品の機能性プロファイリング (2018)
  • Author(s): 藤村 由紀, 立花 宏文.
  • Organizer: 日本農芸化学会西日本支部第322回支部例会
  • Invited
• [Presentation] Green tea polyphenol EGCG upregulates tissue factor pathway inhibitor expression via 67LR signaling. (2018)
  • Author(s): Ren Yoshitomi, Hirokatsu Kanzaki, Takuya Takakura, Sigeyuki Totoki, Motofumi Kumazoe, Yoshinori Fujimura and Hirofumi Tachibana.
  • Organizer: The 31st Annual and International Meeting of Japanese Association for Animal Cell Technolog
  • Int'l Joint Research
• [Presentation] メタボリックプロファイリング法を用いた新緑茶品種「サンルージュ」の認知機能低下抑制作用を担う成分の同定. (2018)
  • Author(s): 佐藤 由佳, 北村 稜, 藤村 由紀, 立花 宏文.
  • Organizer: 第55回化学関連支部合同九州大会
• [Presentation] 緑茶とDiallyl disulfide の機能性フードペアリング. (2018)
  • Author(s): 裵 宰焄, 熊添 基文, 藤村 由紀, 立花 宏文.
  • Organizer: 日本農芸化学会2018年度西日本支部大会
• [Presentation] メタボリックプロファイリング解析による 緑茶品種サンルージュの認知機能低下抑制作用に関与する成分の同定 (2018)
  • Author(s): 北村 稜、野中 遥奈、佐藤 由佳、和才 昌史、村田 希、藤村 由紀、立花 宏文.
  • Organizer: 日本農芸化学会2018年度大会
• [Presentation] 高感度かつ再現性の高い代謝物局在解析を可能とする質量分析イメージングに向けた新たな試料調製法の開発 (2018)
  • Author(s): 一瀬智美、藤村由紀、山崎雄三、割石博之、三浦大典.
  • Organizer: 日本農芸化学会2018年度大会
• [Presentation] MALDI-MS-based High-throughput Metabolite Profiling of Human Body Fluids (2017)
  • Author(s): Miura, D., Kawano, C., Miki, A., Ishii, T., Fujimura, Y., Wariishi, H., and Shindo, M.
  • Organizer: Metabolomics 2017
  • Int'l Joint Research
• [Presentation] Visualization of glucosinolate distribution in the inner structure of the leaf by mass spectrometry imaging (2017)
  • Author(s): Ichinose, T., Murayama, F., Fujimura, Y., Yamazaki, Y., Wariishi, H., Maruyama, A., and Miura, D.
  • Organizer: 65th ASMS Conference on Mass Spectrometry and Allied Topics
  • Int'l Joint Research
• [Presentation] 計量化学的アプローチによる多成分系食品の生理活性評価とその予測を可能とする成分の組合せ選出法の開発 (2017)
  • Author(s): 藤村由紀、河野ちひろ、村山（前田）彩花、早川英介、割石博之、立花宏文、三浦大典.
  • Organizer: 第11回メタボロームシンポジウム
• [Presentation] MALDI-MS代謝物プロファイリング法の開発と緑茶の抗酸化活性評価への応用 (2017)
  • Author(s): 藤村由紀、河野ちひろ、立花宏文、割石博之、三浦大典.
  • Organizer: 第７１回日本栄養・食糧学会大会
• [Presentation] MALDI-MSを用いたヒト体液のハイスループット代謝物プロファイリング (2017)
  • Author(s): 河野ちひろ、 三木晶琴、石井孝典、藤村由紀、 割石博之、 新藤充、三浦大典.
  • Organizer: 第６５回質量分析総合討論会
• [Presentation] 再現性高く簡便かつ高感度な質量分析イメージング試料調製法の確立 (2017)
  • Author(s): 一瀬智美、村山布紗、藤村由紀、山崎雄三、割石博之、三浦大典.
  • Organizer: 第６５回質量分析総合討論会
• [Presentation] 計量化学的技法による多成分系食品の生理活性評価とその予測を可能とする成分コンビネーション選出法の開発 (2017)
  • Author(s): 藤村由紀、河野ちひろ、村山(前田)彩花、早川英介、割石博之、立花宏文、三浦大典.
  • Organizer: 第６５回質量分析総合討論会
• [Presentation] 複合成分系である食品の機能性を高精度に捉えるニュートリメタボロミクス (2017)
  • Author(s): 藤村由紀, 三浦大典, 立花宏文.
  • Organizer: 第39回日本臨床栄養学会総会・第38回日本臨床栄養協会総会 第15回大連合大会
  • Invited
• [Patent(Industrial Property Rights)] がん診断のための代謝バイオマーカー (2019)
  • Inventor(s): 千葉 亨，村田 正治, 藤村 由紀, 他5名.
  • Industrial Property Rights Holder: 千葉 亨，村田 正治, 藤村 由紀, 他5名.
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2019-117258
  • Filing Date: 2019