| Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2020: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2018: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Outline of Final Research Achievements |
Spatial variation in fungal communities in forest soils is high. Entomopathogenic fungi include important natural enemies of forest insects, but their information is limited except during outbreaks, partly because many of them live in soil. The objective of this study was to develop a method for monitoring entomopathogenic fungi in soil using environmental DNA methods. Three different methods were used to extract DNA from 0.4 g (according to a kit recipe) and 15 g (intention to collect fungal DNA more widely) of soil volume. Amplicon analysis was performed using next-generation sequencers. Contrary to expectations, the smaller the amount of soil used for extraction, the greater number of fungal OTU were detected. The results of this study indicate that the best strategy is to sample a large number of small soil samples.
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