• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mechanisms of CWPO-C peroxidase function on the regulation of plant growth, differentiation, and branching

Research Project

Project/Area Number 17H03846
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Wood science
Research InstitutionKyushu University

Principal Investigator

Tsutsumi Yuji  九州大学, 農学研究院, 教授 (30236921)

Co-Investigator(Kenkyū-buntansha) 河岸 洋和  静岡大学, グリーン科学技術研究所, 教授 (70183283)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywordsペルオキシダーゼ / 植物ホルモン / オーキシン / 成長 / 分化 / リグニン / 万能ペルオキシダーゼ / CWPO-C / 分化・成長 / 樹木生長、分化 / 植物ホルモン代謝 / 分枝 / 生長
Outline of Final Research Achievements

The over-expression of CWPO-C (OE), a unique all-round peroxidase, in Arabidopsis were analyzed. The OE mutants showed delay of growth and gravitropism. And IAA contents were largely decreased. The results strongly suggest that CWPO-C catabolizes and inactivates IAA in vivo. This observation was further supported by the in vitro conversion of IAA by rCWPO-C. This finding may give a new insight of IAA catabolism in plant. On the other hand, The OE of CWPO-C in poplar showed the different phenotype, suggesting these two plant species have different regulatory mechanisms in IAA catabolism.
The effect of CWPO-C on the fairy compounds (FCs) those were recently proposed as possible new plant hormones, were also investigated. The CWPO-C over-expression mutant of Arabidopsis attenuated the positive growth effect of the exogenous AOH, one of FCs, and decrease endogenous AOH. It suggests that CWPO-C may also related to the metabolism and regulation of FCs in plant.

Academic Significance and Societal Importance of the Research Achievements

IAAの代謝不活化機構については、未解明の部分が残されておりIAAオキシダーゼによるOxIAAへの代謝変換やアミノ酸抱合体やグルコース抱合体への変換などが提案されている。一方本研究において、万能ペルオキシダーゼCWPO-C過剰発現体の表現型は、IAA欠乏状態の表現型とよく一致し、内生IAA量が著しく低下していたこと、組換体CWPO-CによるIAA代謝が確認されたことからCWPO-Cによる新たなIAA代謝経路が示唆された。IAAは植物の成長や分岐を制御する重要な因子であり、生産性と価値に大きな影響を持つため、その作用メカニズム解明は植物生産において非常に重要な知見になりうる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (11 results)

All 2020 2019 2018 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (10 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] Expression analysis of transporter genes for screening candidate monolignol transporters using Arabidopsis thaliana cell suspensions during tracheary element differentiation2017

    • Author(s)
      Takeuchi Manami、Kegasa Takahiro、Watanabe Atsushi、Tamura Miho、Tsutsumi Yuji
    • Journal Title

      J plant Res

      Volume: 131 Issue: 2 Pages: 297-305

    • DOI

      10.1007/s10265-017-0979-4

    • NAID

      40021495040

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] 万能ペルオキシダーゼ、 CWPO-Cによる植物の成長制御とオーキシン代謝2020

    • Author(s)
      横山 裕亮、富家 梓、YOSHIKAY Diego、雉子谷 佳男、堤 祐司
    • Organizer
      第70回日本木材学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] モウソウチク(Phyllostachys pubescens)稈由来リグニンの分解生成物中に含まれる化合物の生理活性2020

    • Author(s)
      廣島 将大、栫 拓巳、堤 祐司、清水 邦義
    • Organizer
      第70回日本木材学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 細胞壁二次壁構造を模倣した三次元マトリックスフィルムの創製2020

    • Author(s)
      川口 なつみ、堤 祐司
    • Organizer
      第70回日本木材学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Regulation of differentiation and growth by plant peroxidase CWPO-C2019

    • Author(s)
      Diego A. Yoshikay, Yusuke Yokoyama , Jun Shigeto, Yuji Tsutsumi
    • Organizer
      1st International Lignin Symposium
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cytotoxicity of lignin-derived compounds isolated from bamboo (Phyllostachys pubescence) on cancer cell line2019

    • Author(s)
      Hiroshima Shota, Kakoi Takumi, Hayashi Junya, Tsutsumi Yuji, Shimizu Kuniyoshi Shimizu
    • Organizer
      1st International Lignin Symposium
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 植物ペルオキシダーゼ、CWPO-C による成長制御作用機序の解明2019

    • Author(s)
      横山裕亮 、富家 梓、Diego Yoshikay、雉子谷佳男、堤 祐司
    • Organizer
      第26回日本木材学会九州支部大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 植物ペルオキシダーゼ、CWPO-Cによる分化成長の制御2019

    • Author(s)
      横山 裕亮、重藤 潤、大平 香織、堤 祐司
    • Organizer
      第69回日本木材学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Generation of lignin polymer models via dehydrogenative polymerization of coniferyl alcohol and sinapyl alcohol via plant peroxydase involved in lignification and analysis of the resulting DHPs by MALDI-TOF analysis2018

    • Author(s)
      Yuji Tsutsumi, Jun Shigeto, Hiroki Honjo
    • Organizer
      29th International Conference of Polyphenols
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The contribution of CWPO-C to primary stage of plant growth and organogenesis2018

    • Author(s)
      Diego Yoshikay, Jun Shigeto, Yusuke Yokoyama, Yuji Tsutsumi
    • Organizer
      5th Symposium of Biotechnology Applied to Lignocelluloses
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シロイヌナズナにおけるリグニンモノマー輸送体遺伝子の探索2018

    • Author(s)
      武内 真奈美、堤 祐司
    • Organizer
      第68回日本木材学会大会
    • Related Report
      2017 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi