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Characteristic physiology and ecology of a protozoan parasite Perkinsus olseni prevailing in wild stocks of Manila clam

Research Project

Project/Area Number 17H03858
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Aquatic bioproduction science
Research InstitutionThe University of Tokyo

Principal Investigator

Itoh Naoki  東京大学, 大学院農学生命科学研究科(農学部), 准教授 (30502736)

Co-Investigator(Kenkyū-buntansha) 良永 知義  東京大学, 大学院農学生命科学研究科(農学部), 教授 (20345185)
高橋 計介  東北大学, 農学研究科, 准教授 (80240662)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2019: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywords原虫 / 二枚貝類 / 生体防御 / 宿主特異性 / 培養 / 栄養要求性 / 宿主寄生虫間相互関係 / Perkinsus olseni / アサリ / 寄生虫 / 魚病 / 生理 / 寄生 / in vitro培養 / 血球
Outline of Final Research Achievements

A protozoan parasite Perkinsus olseni is prevailing in wild stocks of Manila clam in Japan, and this research project aimed at understanding physiological and ecological adaptation of P. olseni to the host species. In this study, P. olseni were characterized physiologically and ecologically, and we clarified that the proliferation of P. olseni was not inhibited by host bivalves, and that production of the major infective stage increased in warmer season when mass mortality of Manila clams often occurs. These characters are considered to be parts of advantages for P. olseni to prevail in Manila clam stocks in Japan. We also examined host specificity and geographical distribution, and established an in vitro isolate of the related comparative species, P. beihaiensis, in Japan. In future, these information and research materials would be useful to reveal further physiological and ecological adaptation of P. olseni to the host species.

Academic Significance and Societal Importance of the Research Achievements

Perkinsus属原虫は重要水産生物である二枚貝生産を脅かす寄生生物であり、本研究対象であるP. olseniは国際獣疫事務局による指定を受けた重要病原体である。本研究では本種が国内アサリ資源への蔓延に至った生理・生態学的特徴の一端を解明することに成功、加えて研究ツールの開発や新たな生活サイクルの提唱にも至った。これらの成果は本種による疾病を克服し安定的な二枚貝生産へ貢献すると考えられる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (9 results)

All 2020 2019 2018 2017

All Journal Article (4 results) (of which Peer Reviewed: 3 results) Presentation (5 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results)

  • [Journal Article] The effects of environmental and nutritional conditions on the development of Perkinsus olseni prezoosporangia2020

    • Author(s)
      Umeda Kousuke、Yang Xia、Waki Tsukasa、Yoshinaga Tomoyoshi、Itoh Naoki
    • Journal Title

      Experimental Parasitology

      Volume: 209 Pages: 107827-107827

    • DOI

      10.1016/j.exppara.2019.107827

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] First discovery of Perkinsus beihaiensis in Mediterranean mussels (Mytilus galloprovincialis) in Tokyo Bay, Japan2019

    • Author(s)
      Itoh Naoki、Komatsu Yoshiki、Maeda Kazuki、Hirase Shotaro、Yoshinaga Tomoyoshi
    • Journal Title

      Journal of Invertebrate Pathology

      Volume: 166 Pages: 107226-107226

    • DOI

      10.1016/j.jip.2019.107226

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] New insights into the entrance of Perkinsus olseni in the Manila clam, Ruditapes philippinarum2018

    • Author(s)
      Wang Yu、Yoshinaga Tomoyoshi、Itoh Naoki
    • Journal Title

      Journal of Invertebrate Pathology

      Volume: 153 Pages: 117-121

    • DOI

      10.1016/j.jip.2018.03.005

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 二枚貝寄生原虫と宿主生理との関係2017

    • Author(s)
      伊藤直樹
    • Journal Title

      日本水産学会誌

      Volume: 83 Pages: 833-833

    • Related Report
      2017 Annual Research Report
  • [Presentation] 宿主因子を含まないPerkinsus olseniの前遊走子嚢作出培地の開発2019

    • Author(s)
      前田和輝、良永知義、伊藤直樹
    • Organizer
      令和元年度日本魚病学会秋季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] NOVEL INSIGHTS IN DIFFERENTIATION OF Perkinsus olseni TROPHOZOITES INTO PREZOOSPORANGIA2019

    • Author(s)
      Xia Yang, Kousuke Umeda, Tsukasa Waki, Tomoyoshi Yoshinaga, Naoki Itoh
    • Organizer
      Aquacultture 2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Studies for Infectious Diseases of Aquatic Organisms2018

    • Author(s)
      Naoki Itoh
    • Organizer
      E3-start 2018: International Academic R&D Festival
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] EXPERIMENTAL INFECTION TO ASSESS PATHOGENICITY OF PERKINSUS BEIHAIENSIS AGAINST DOMESTIC BIVALVE SPECIES IN JAPAN2018

    • Author(s)
      Yoshiki Komatsu, Tomoyoshi Yoshinaga, Naoki Itoh
    • Organizer
      110th Annual meeting of National Shellfisheries Association
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ESTABLISHMENT OF Perkinsus beihaiensis ISOLATE FROM THE MEDITERRANEAN MUSSEL (Mytilus galloprovincialis) IN JAPAN2017

    • Author(s)
      Yoshiki Komatsu, Tomoyoshi Yoshinaga, Naoki Itoh
    • Organizer
      10th Symposium on Diseases in Asian Aquaculture
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research

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Published: 2017-04-28   Modified: 2021-02-19  

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