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New approach for classification of scleractinian coral by molecular cytogenetics

Research Project

Project/Area Number 17H03861
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Aquatic bioproduction science
Research InstitutionKochi University

Principal Investigator

Taguchi Takahiro  高知大学, 教育研究部総合科学系黒潮圏科学部門, 短期研究員 (80127943)

Co-Investigator(Kenkyū-buntansha) 久保田 賢  高知大学, 教育研究部総合科学系黒潮圏科学部門, 教授 (00314980)
目崎 拓真  公益財団法人黒潮生物研究所, 研究部局, 研究所長 (20840482)
深見 裕伸  宮崎大学, 農学部, 教授 (50402756)
新里 宙也  東京大学, 大気海洋研究所, 准教授 (70524726)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2017: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Keywords分類・形態 / 分子細胞遺伝学 / FISH
Outline of Final Research Achievements

In this study, we mainly focused Acropora pruinosa and Acropora tumida, which had no distinction between morphological classification and molecular classification.
Molecular cytogenetic techniques which were developed by our laboratory were applied for chromosome analysis of scleractinian corals.
It was revealed that they are heterogeneous by finding differences in the loci of ribosomal RNA genes and the presence or absence of sex chromosome candidates.

Academic Significance and Societal Importance of the Research Achievements

造礁サンゴの種の同定には,主に形態およびDNA配列が広く用いられてきたが,種レベルでは形態的に異なっているにも関わらず遺伝的にほぼ同じという例や,形態的に同じであるにもかかわらず遺伝的に離れている例が多く見られ,種の同定は容易ではなかった。本研究では,染色体解析(核型分析)や蛍光in situハイブリダイゼーション(FISH)法を適用した分子細胞遺伝学的解析により,造礁サンゴの種判別における染色体解析の有効性を示すことができた。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (4 results)

All 2018 2017

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (2 results)

  • [Journal Article] Molecular Cytogenetic Analysis and Isolation of a 5S rRNA-Related Marker in the Scleractinian Coral Platygyra contorta Veron 1990 (Hexacorallia, Anthozoa, Cnidaria)2017

    • Author(s)
      Takahiro Taguchi, Satoshi Kubota, Erika Tagami, Takuma Mezaki, Satoko Sekida, Kazuo Okuda, Akira Tominaga
    • Journal Title

      Cytologia

      Volume: 82 Pages: 205-212

    • NAID

      130006769798

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Recent Progress of Molecular Cytogenetic Study on Scleractinian (Stony) Corals.2017

    • Author(s)
      Takahiro Taguchi, Erika Tagami, Takuma Mezaki, Satoko Sekida, Yalan Chou, Keryea Soong, Kazuo Okuda, Akira Tominaga, Satoshi Kubota
    • Journal Title

      Kuroshio Science

      Volume: 11-1 Pages: 73-81

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] エダミドリイシとヒメエダミドリイシの染色体比較解析2018

    • Author(s)
      田口尚弘、目崎拓真、奥村正俊、久保田賢
    • Organizer
      日本サンゴ礁学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 造礁サンゴ染色体研究の進展2017

    • Author(s)
      田口尚弘,目崎拓真,富永明,久保田賢
    • Organizer
      日本サンゴ礁学会第20回大会
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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